Abstract:Objective To construct a personalized discharge readiness assessment system for stroke patients based on the Omaha System,in order to implement targeted peri-discharge management and improve the quality of life of patients.Methods Through systematic literature review,a draft of the"Discharge Readiness Assessment System for Stroke Patients"covering basic framework and personalized indicators was formed.Fifteen experts from the fields of neurology and neurosurgery nursing,neurology medical care,health management,neurorehabilitation,and nursing education were selected to conduct two rounds of Delphi consultation using the Delphi method.Expert opinions were analyzed and discussed,and the draft was modified to determine the final personalized assessment system.Results The expert authority scores in the two rounds of consultation were 0.94 and 0.96,respectively,with a response rate of 100%.The Kendall's coefficient of concordance was 0.24 and 0.21(P<0.05),respectively.The final"Discharge Readiness Assessment System for Stroke Patients"includes 4 first-level indicators(physiological assessment,psychosocial assessment,health-related behavior assessment,and environmental assessment),11 second-level indicators,and 37 third-level indicators.The importance scores of each indicator range from 4.0 to 5.0,and the coefficient of variation ranges from 0.00 to 0.23.Conclusion The"Discharge Readiness Assessment System for Stroke Patients"constructed based on the Omaha System can accurately screen the personalized needs of stroke patients from a physiological-psychological-social perspective,providing a basis for targeted peri-discharge management of stroke patients.

Abstract:Objective To analyze the research hotspots and frontier trends in the field of dry eye syndrome treatment at home and abroad,and provide references for future research.Methods Relevant literature was retrieved from the China National Knowledge Infrastructure(CNKI)and the Web of Science(WoS)core collection,and data mining and analysis were conducted using CiteSpace software.Result A total of 2925 Chinese and 3813 English articles were included.The research hotspots and frontier trends mainly focused on the physiological and pathological mechanisms of the ocular surface,drug treatment,traditional Chinese medicine therapy,and efficacy evaluation.Conclusion The research on dry eye syndrome treatment is generally on the rise,but the exploration of new research directions in this field in China is slightly slower than that in foreign countries.China can explore multi-faceted ideas in the field of dry eye syndrome treatment by drawing on the research hotspots and frontier trends of foreign countries,so as to further optimize the treatment strategies for dry eye syndrome.

Abstract:Objective To analyze the influencing factors of early neurological deterioration(END)after intravenous thrombolysis in patients with acute ischemic stroke(AIS)and to construct a prediction model.Methods A total of 108 AIS patients who received thrombolysis treatment at Nanyang Central Hospital from April 2022 to April 2025 were selected as the study subjects(training set).All patients received the same standard intravenous thrombolysis treatment and subsequent management.The occurrence of END and general data after thrombolysis were collected.According to whether END occurred within 24 hours after thrombolysis,the patients were divided into the END group and the non-END group.Univariate and multivariate Logistic regression analysis were used to screen the independent risk factors for END,and a prediction model was constructed based on these factors.The receiver operating characteristic(ROC)curve was used to evaluate the discrimination of the model,and the Hosmer-Lemeshow test and calibration curve were used to evaluate the calibration of the model.Another 100 AIS patients were collected to externally validate the model performance.Results Among the 108 AIS patients after thrombolysis,30 cases developed END,with an incidence rate of 27.78%(30/108),and were included in the END group;78 cases did not develop END and were included in the non-END group.The proportion of patients with atrial fibrillation,baseline National Institutes of Health stroke scale(NIHSS)score,red blood cell distribution width(RDW),and serum galectin-3(Gal-3)level in the END group were all higher than those in the non-END group,and the differences were statistically significant(P<0.05).Multivariate Logistic regression analysis showed that atrial fibrillation,baseline NIHSS score,RDW,and elevated Gal-3 level were all independent risk factors for END after thrombolysis in AIS patients(P<0.05).Four risk factors from the multivariate results were used as predictive factors to construct a prediction model.The Hosmer-Lemeshow test showed that the P values for the training set and validation set were 0.415 and 0.533,respectively,indicating that the model had good consistency in predicting END after thrombolysis in AIS patients for both the training set and validation set.Conclusion Atrial fibrillation,baseline NIHSS score,RDW,and elevated Gal-3 level are all independent risk factors for END after thrombolysis in AIS patients.The prediction model established based on these variables has good clinical predictive efficacy.

Abstract:Objective To analyze the influencing factors of deep vein thrombosis of the lower extremities(DVT)after cesarean section in patients with preeclampsia.Methods A retrospective analysis was conducted on 402 patients with preeclampsia who underwent cesarean section for delivery at Zhengzhou Shengma Maternity Hospital from January 2023 to December 2024.The occurrence of DVT after cesarean section was recorded,and the patients were divided into an occurrence group and a non-occurrence group based on the occurrence of DVT.The clinical data of the two groups were compared,and a multivariate Logistic regression model was constructed to analyze the influencing factors of DVT after cesarean section in patients with preeclampsia.Results Among the 402 patients with preeclampsia who underwent cesarean section,23 developed DVT after the operation,with an incidence rate of 5.72%(23/402).Univariate analysis showed that the proportion of patients aged≥35 years and with a history of thrombosis in the occurrence group were higher those that in the non-occurrence group,the postpartum bed rest time was longer than that in the occurrence group,and the levels of serum triglycerides(TG),total cholesterol(TC),and plasma D-dimer(D-D)were higher than those in the occurrence group,with statistically significant differences(P<0.05).Multivariate Logistic regression analysis showed that age≥35 years,long postpartum bed rest time,history of thrombosis,elevated TG,TC,and D-D levels were all risk factors for DVT after cesarean section(OR>1,P<0.05).Conclusion age≥35 years,long postpartum bed rest time,history of thrombosis,elevated TG,TC,and D-D levels are all risk factors for DVT after cesarean section.Attention should be paid to pregnant women with risk factors,and early prevention work should be done well.

Abstract:Objective To compare the treatment efficacy and safety of two anti-infective regimens for early-onset neonatal sepsis(EONS)based on real-world data.Methods A retrospective study was conducted on the clinical data of 268 neonates diagnosed with EONS at Shenzhen Second People's Hospital from August 2022 to December 2023.The neonates were divided into the piperacillin-tazobactam(PT)group and the penicillin combined with ceftazidime(PC)group based on different treatment regimens.The treatment efficacy,length of hospital stay,duration of antibiotic use>5 days,and adverse reactions were observed and compared between the two groups.Multivariate regression analysis was used to correct for baseline imbalances.Results The total treatment efficacy rate of the PT group was 96.2%,which was higher than that of the PC group(90.2%).After adjusting for gestational age,mode of delivery,abnormal white blood cell count(WBC),premature rupture of membranes(PROM)>18 hours,and positive screening for Group B Streptococcus in pregnant mothers,the multivariate regression analysis showed that the adjusted clinical efficacy rate of the PT group was 2.4 times that of the PC group(OR=2.4,95%CI:0.78-7.34),but the difference was not statistically significant(P=0.125).Gestational age was a significant factor affecting the treatment efficacy(OR=4.9,95%CI:1.64-14.68,P=0.004).There was no significant difference in the length of hospital stay>5 days(OR=1.09,95%CI:0.66-1.81,P=0.725)and the duration of antibiotic use>5 days(OR=0.90,95%CI:0.53-1.54,P=0.702)between the two groups.There was no statistically significant difference in the occurrence of adverse reactions between the two groups(P>0.05).Conclusion Piperacillin-tazobactam may have better clinical efficacy than penicillin combined with ceftazidime in the treatment of neonates≥35 weeks with EONS.Full-term infants have better clinical outcomes than preterm infants with EONS,and there is no increase in adverse reactions.

Abstract:Objective To explore the improvement effect of Roxadustat combined with recombinant human erythropoietin(rHuEPO)and iron sucrose on anemia status and microinflammation in maintenance hemodialysis(MHD)patients.Methods A total of 100 MHD patients with renal anemia admitted to The First Affiliated Hospital of Longyan Fujian Medical University from January 2022 to January 2025 were selected and randomly divided into a control group and an observation group,with 50 cases in each group.Patients in the control group were treated with subcutaneous injection of rHuEPO combined with intravenous infusion of iron sucrose,while patients in the observation group were treated with oral Roxadustat on the basis of the control group.Both groups were treated for 3 months.The clinical efficacy,anemia-related indicators[hemoglobin(Hb),hematocrit(Hct),serum ferritin(SF),transferrin saturation(TSAT)],microinflammation indicators[high-sensitivity C-reactive protein(hs-CRP),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)],and adverse reactions were compared between the two groups before and after treatment.Results The total effective rate of the observation group was higher than that of the control group,and the difference was statistically significant(P<0.05).After treatment,the levels of Hb,Hct,SF,and TSAT in both groups were higher than those before treatment,and the levels in the observation group were higher than those in the control group.The levels of serum hs-CRP,IL-6,and TNF-α were lower than those before treatment,and the levels in the observation group were lower than those in the control group.The differences were statistically significant(P<0.05).There was no statistically significant difference in the total incidence of adverse reactions between the two groups(P>0.05).Conclusion Roxadustat combined with rHuEPO and iron sucrose can effectively correct anemia in MHD patients,improve iron metabolism levels,and reduce microinflammation,without increasing the risk of adverse reactions.

Abstract:Objective Meta-analysis was used to systematically evaluate the efficacy and safety of Tongxinluo Capsules in the treatment of coronary slow flow(CSF).Methods Randomized controlled trials(RCT)on the combination of Tongxinluo capsules and conventional Western medicine for the treatment of CSF were retrieved from China National Knowledge Infrastructure(CNKI),VIP,WanFang,China Biology Medicine Disc(CBM),PubMed,EMbase,and Cochrane Library databases from their establishment to December 31,2024.Two researchers independently screened the literature,extracted data,and evaluated the risk of bias in the included studies.Meta-analysis was conducted using"RevMan 5.3"software.Results A total of 11 RCT involving 840 patients were included.The Meta-analysis results showed that compared with conventional Western medicine alone,the combination of Tongxinluo capsules and conventional Western medicine could further increase the total clinical effective rate[relative risk(RR)=1.61,95%CI:1.44-1.81,P<0.01],improve coronary blood flow velocity[mean difference(MD)=-9.21,95%CI:-12.88--5.54,P<0.01],increase adiponectin(APN)levels(MD=2.42,95%CI:2.22-2.62,P<0.01),and reduce serum high-sensitivity C-reactive protein(hs-CRP)levels(MD=-1.35,95%CI:-1.49--1.21,P<0.01).There was no statistically significant difference in the incidence of adverse reactions between the two groups(RR=1.07,95%CI:0.32-3.51,P=0.92).Conclusion The current evidence suggests that the combination of Tongxinluo capsules and conventional Western medicine is more effective in the treatment of CSF than conventional Western medicine alone,without increasing the incidence of adverse reactions.

Abstract:Objective To investigate the iodine nutritional status of children aged 8 to 10 in Licheng District from 2020 to 2024,to evaluate the prevention and control effect of iodine deficiency disorders(IDD)in this area,and to provide reliable basis for further optimizing the prevention and control strategies.Methods From 2020 to 2024,one primary school was randomly selected from each of the five districts(divided according to the east,west,south,north,and center directions)in Quanzhou Licheng District.At least 40 children aged 8 to 10 who were day students were selected from each school,totaling 1031 children as the research subjects.Random urine samples from all children were collected for urine iodine content detection,and salt samples from their homes were collected for salt iodine content detection to investigate the iodine nutritional status of the children.At the same time,thyroid B-ultrasound examinations were conducted to measure the thyroid volume of all children and assess the rate of goiter.Results The coverage rates of iodized salt in 2020,2021,2022,2023,and 2024 were 93.00%,93.27%,92.86%,95.65%,and 97.09%respectively;the qualified rates of iodized salt were 96.77%,96.39%,97.95%,98.48%,and 99.00%respectively;the rates of consumption of qualified iodized salt were 90.00%,89.90%,90.95%,94.20%,and 96.12%respectively;the rates of non-iodized salt were 7.00%,6.73%,7.14%,4.35%,and 2.91%respectively.The median urinary iodine(MUI)of children in Licheng District from 2020 to 2024 was 189.28 μg·L-1,among which 57.23%was adequate,5.53%was mild deficiency,12.90%was moderate deficiency,1.07%was severe deficiency,14.84%was above adequate,and 8.44%was excessive.A total of 28 cases of goiter occurred among 1031 children in the five years,with a total incidence rate of 2.72%.The incidence rates of goiter in different genders and different ages were similar(χ2=0.176,1.594,P=0.675,0.451).Conclusion The iodine nutritional status of children aged 8 to 10 in Licheng District is good,and the national elimination standard of IDD has been basically achieved.However,there are still a small number of children with insufficient iodine intake,and it is necessary to strengthen the market supervision of iodized salt supply and enhance the publicity and education on IDD for residents.

Abstract:Objective To observe the effect of seamless connection between pre-hospital emergency care and chest pain center on improving the rescue success rate of patients with acute ST-segment elevation myocardial infarction.Methods A retrospective analysis was conducted on 99 patients with acute ST-segment elevation myocardial infarction admitted to Zhangzhou Municipal Hospital from November 2020 to November 2022.The patients were divided into a control group and an observation group based on the admission time and the different emergency measures.The 49 patients with acute myocardial infarction who received traditional pre-hospital emergency care from November 2020 to November 2021 were set as the control group,and the 50 patients with acute myocardial infarction who received pre-hospital emergency care and seamless connection with the chest pain center from December 2021 to November 2022 were set as the observation group.The time to establish intravenous access,the time from first medical contact(FMC)to the first electrocardiogram,the time from hospital entrance to balloon(D-to-B)opening,and the rescue success rate were compared between the two groups.Results The time to establish intravenous access,the time from FMC to the first electrocardiogram,and the D-to-B opening time of the observation group were shorter than those of the control group,and the differences were statistically significant(P<0.05);the rescue success rate of the observation group was higher than that of the control group,and the difference was statistically significant(P<0.05).Conclusion Seamless connection between pre-hospital emergency care and chest pain center has a good rescue effect in the emergency treatment of patients with acute ST-segment elevation myocardial infarction,which can shorten the time to establish intravenous access,the time from FMC to the first electrocardiogram,and the D-to-B opening time,and improve the rescue success rate.

Abstract:Objective The efficacy and safety of traditional Chinese medicine compound combined with Western medicine in the treatment of gynecomastia(GYN)were systematically evaluated by using the meta-analysis method.Methods Chinese and English databases such as PubMed,China National Knowledge Infrastructure(CNKI),Weipu,and Wanfang were retrieved to screen out randomized controlled trials(RCT)of traditional Chinese medicine compound prescriptions combined with Western medicine for the treatment of GYN.The retrieval period was from the establishment of the databases to March 2025,and methodological quality evaluation was conducted.Statistical graphs were drawn using"Revman 5.4"software for meta-analysis,sensitivity analysis and analysis of publication bias.Results A total of 7 RCTs were included in this study,involving 550 patients,with 282 cases in an observation group and 268 cases in a control group.The results showed that the total effective rate and cure rate of GYN treated with traditional Chinese medicine compound combined with Western medicine were both higher than those treated with Western medicine alone(OR=6.99,95%CI:4.12-11.85,P<0.000 01;OR=3.84,95%CI 2.69-5.49,P<0.000 01).The ineffective rate was significantly lower than that of treatment with Western medicine alone(OR=0.14,95%CI:0.08-0.24,P<0.000 01),however,there was no statistically significant difference in the comparison of effective rates between the two regimens(OR=0.78,95%CI:0.54-1.12,P=0.18),and no adverse reactions were reported in the clinical trials.Conclusion The total effective rate and cure rate of traditional Chinese medicine compound combined with Western medicine in the treatment of GYN are significantly higher than those of Western medicine alone,and it does not increase adverse reactions.However,due to the limitations of the number and quality of included studies,large-sample prospective studies should still be conducted to confirm this conclusion.

Abstract:Objective To explore the effect of epinephrine combined with budesonide in the treatment of acute laryngitis complicated with laryngeal obstruction in children.Methods A total of 100 children with acute laryngitis complicated with laryngeal obstruction admitted to Luoyang Maternal and Child Health Hospital from January 2023 to December 2023 were selected as the research subjects and randomly divided into a control group and an observation group,with 50 cases in each group.Both groups of children received conventional symptomatic and supportive treatment.The control group was treated with budesonide,while the observation group was treated with epinephrine combined with budesonide.The clinical efficacy,time to disappearance of main symptoms,serum inflammatory factor levels before and after treatment,and adverse reactions were compared between the two groups.Results The total effective rate of the observation group was 92.00%,which was higher than 76.00%of the control group,and the difference was statistically significant(P<0.05).The time to disappearance of stridor,dyspnea,barking cough and hoarseness in the observation group was shorter than that in the control group,and the difference was statistically significant(P<0.05).After treatment,the levels of serum interleukin-6(IL-6)and procalcitonin(PCT)in both groups were lower than those before treatment,and the levels in the observation group were lower than those in the control group,and the differences were statistically significant(P<0.05).There was no statistically significant difference in the total incidence of adverse reactions between the two groups(P>0.05).Conclusion Epinephrine combined with budesonide is effective in the treatment of acute laryngitis complicated with laryngeal obstruction in children,achieving a synergistic effect,shortening the time to disappearance of main symptoms,reducing inflammatory response,and not increasing adverse reactions.

Abstract:Objective To analyze and explore the application value of the flipped classroom teaching model in nursing education.Methods This study adopted a historical control research design.A total of 189 vocational college nursing students from a tertiary hospital of Zhangzhou from November 2017 to November 2019 were selected as a control group,and 192 vocational college nursing students from the same department from January 2020 to January 2022 were selected as an observation group.Both groups of vocational college nursing students received the same teaching content and frequency.The control group of vocational college nursing students adopted the traditional teaching model,while the observation group adopted the flipped classroom teaching model.The examination scores,satisfaction with the nursing teaching model,and self-study ability before and after teaching of the two groups of vocational college nursing students were compared.Results The theoretical and practical scores of the vocational college nursing students in the observation group were higher than those in the control group,and the differences were statistically significant(P<0.05);the total satisfaction rate of the vocational college nursing students in the observation group with the nursing teaching model was higher than that in the control group,and the difference was statistically significant(P<0.05);after teaching,the scores of each dimension of the vocational college nursing students in the observation group were higher than those in the control group,and the differences were statistically significant(P<0.05).Conclusion The application of the flipped classroom teaching model has significant effects,which can effectively stimulate the learning enthusiasm of vocational college nursing students and improve teaching satisfaction.

Abstract:Objective To analyze the key factors influencing the total hospitalization costs of hysteroscopic surgery under the diagnosis related groups(DRG)payment model,and to provide a basis for optimizing cost control and promoting the rational allocation of resources.Methods A retrospective collection of data from 3903 patients who underwent hysteroscopic surgery at a hospital in Guangzhou from June 2021 to June 2025 was conducted.The total hospitalization cost was taken as the dependent variable,and age,postoperative diagnosis,and surgical methods were taken as independent variables for univariate analysis and multiple linear regression analysis.Results The average age of the 3903 patients was(41.8±10.4)years.Univariate analysis showed that there were statistically significant differences in costs among different ages,different diseases,and different surgical methods(P<0.001).Multiple linear regression(adjusted R²=0.542)indicated that the surgical method was the strongest predictor of cost,with the cost of hysteroscopic endometrial/myoma resection increasing by 153.2%compared to the reference group.Compared with patients under 45 years old,those aged 45 to 60 years had a significant 6.9%reduction in costs.Specific postoperative diagnoses(such as ectopic pregnancy and intrauterine adhesions)were also independent influencing factors for costs(P<0.05).Conclusion Under the DRG payment background,the total hospitalization cost of hysteroscopic surgery is mainly driven by the complexity of the surgical method,and the age of the patient and postoperative diagnosis also have significant influences.It is suggested that in the DRG grouping and weight setting,the complexity of the surgery and patient characteristics should be considered more precisely to establish a fairer and more reasonable compensation mechanism.

Abstract:Objective To analyze the influencing factors of early miscarriage after in vitro fertilization-embryo transfer(IVF-ET)in infertile patients with ovarian endometriomas(OEM)and to construct a risk prediction model.Methods The clinical data of 142 infertile patients with OEM who were treated at Luoyang Central Hospital from January 2022 to February 2025 were retrospectively analyzed.All patients successfully conceived after IVF-ET and were divided into an occurrence group(20 cases)and a non-occurrence group(122 cases)based on whether early miscarriage occurred.The clinical data of the two groups were compared to analyze the influencing factors of early miscarriage after IVF-ET.On this basis,a risk prediction model was constructed and its predictive efficacy was evaluated.Results There were statistically significant differences in pre-assisted reproductive technology body mass index(BMI),proportion of operations different uterine cavity histories,basal estradiol level,and β-human chorionic gonadotropin(β-HCG)level 14 days after embryo transfer between the two groups(P<0.05).Multivariate Logistic regression analysis showed that pre-assisted reproductive technology BMI<18.5 kg·m-2,pre-assisted reproductive technology BMI>24.0 kg·m-2,history of uterine cavity operation>1 time,and decreased β-HCG level 14 days after embryo transfer were risk factors for early miscarriage after IVF-ET in infertile patients with OEM(P<0.05).Based on the above indicators,a risk prediction model for early miscarriage after IVF-ET in infertile patients with OEM was constructed.The calibration curve indicated that the model had good discrimination,and the area under the curve(AUC)was 0.918,95%CI:0.869-0.966.Conclusion Pre-assisted reproductive technology BMI<18.5 kg·m-2,pre-assisted reproductive technology BMI>24.0 kg·m-2,history of uterine cavity operation>1 time,and decreased β-HCG level 14 days after embryo transfer are risk factors for early miscarriage after IVF-ET in infertile patients with OEM.The nomogram model constructed based on these factors has high value in predicting early miscarriage after IVF-ET in infertile patients with OEM.

Abstract:Objective To observe the effects of curcumin on the proliferation,migration and metastasis of human breast cancer MCF-7 cells and explore the mechanism of the PI3K/AKT/mTOR signaling pathway.Methods The proliferation activity of human breast cancer MCF-7 cells was detected by cell counting kit-8(CCK-8)assay after intervention with different concentrations of curcumin.The invasion ability of MCF-7 cells was evaluated by Transwell assay.The phosphorylation status of p-AKT and p-mTOR in the signaling pathway of human breast cancer MCF-7 cells was analyzed by Western blotting.Results Compared with the blank control group,the proliferation and invasion inhibition ability of human breast cancer MCF-7 cells in different concentrations of curcumin groups were significantly reduced,and the differences were statistically significant(P<0.05).Compared with the blank control group,curcumin could up-regulate the expression of E-cadherin in human breast cancer MCF-7 cells,down-regulate the expression of N-cadherin and Vimentin and inhibit the phosphorylation levels of p-AKT and p-mTOR(P<0.05),and this effect showed a significant dose-time dependent relationship.Conclusion Curcumin can inhibit the growth of human breast cancer MCF-7 cells,weaken the invasion ability and increase the apoptosis rate of cells,and the mechanism may be related to the inhibition of the PI3K/AKT/mTOR signaling pathway by curcumin.

Abstract:Objective To explore the related factors influencing the prognosis of patients with chronic rhinosinusitis(CRS)after endoscopic sinus surgery.Methods A retrospective analysis was conducted on the clinical data of 70 CRS patients who underwent endoscopic sinus surgery at Xinyang Traditional Chinese Medicine Hospital from January 2023 to December 2024.The patients were divided into a recurrence group and a non-recurrence group based on whether they had a recurrence within 6 months of follow-up.The clinical data of the two groups were compared,and the indicators with significant differences were subjected to Logistic regression analysis to explore the factors influencing the prognosis recovery of CRS patients after endoscopic sinus surgery.Results A total of 70 CRS patients were included in this study,among whom 15 had a recurrence,accounting for 21.43%,and were included in the recurrence group;55 did not have a recurrence,accounting for 78.57%,and were included in the non-recurrence group.There were statistically significant differences in the percentage of peripheral eosinophils(EOS),preoperative severity score of the disease,the proportion of long-term use of nasal decongestants in the past,and the proportion of regular medication and dressing changes after the operation(P<0.05).Multivariate Logistic regression analysis showed that a history of long-term use of nasal decongestants,no regular medication or dressing change after the operation,elevated peripheral EOS percentage,the preoperative condition was serious were independent risk factors for recurrence in CRS patients(OR=8.396,6.714,6.842,1.766,P<0.05).Conclusion A history of long-term use of nasal decongestants,no regular medication or dressing change after the operation,elevated peripheral EOS percentage,and the preoperative condition are independent risk factors for recurrence in CRS patients.By analyzing these factors,high-risk groups can be accurately screened out,providing strong support for the formulation of clinical prevention and treatment plans.

Abstract:Objective To retrospectively study and analyze the application effect of intraoperative autologous blood transfusion in elective aortic dissection surgery.Methods The general data and preoperative and postoperative clinical laboratory indicators of 104 patients who underwent elective aortic dissection surgery during hospitalization at The First Affiliated Hospital of Naval Medical University,Chinese People's Liberation Army from January 2018 to December 2022 were retrospectively analyzed.The effect of intraoperative autologous blood transfusion on reducing the amount of allogeneic blood transfusion during surgery was observed,and the correlation between the amount of allogeneic blood transfusion and the amount of autologous blood recovery was analyzed by multiple linear regression.Results The average intraoperative blood loss of the patients was(1910.45±976.56)mL,the amount of autologous blood transfusion was(790.01±526.03)mL,the amount of allogeneic blood used was(680.77±659.81)mL,and the cardiopulmonary bypass time was(147.49±44.31)min.The hemoglobin(Hb)level of the postoperative patients was lower than that before the operation,the hematocrit(Hct)was smaller than that before the operation,the platelet(PLT)count was less than that before the operation,and the activated partial thromboplastin time(APTT)and prothrombin time(PT)were longer than those before the operation.The differences were all statistically significant(P<0.05).Multiple linear regression analysis showed that the amount of allogeneic blood transfusion during surgery was positively correlated with intraoperative blood loss(β=0.810,P<0.001)and negatively correlated with the amount of autologous blood recovery(β=-0.460,P<0.001).Conclusion Intraoperative autologous blood transfusion is an important protective factor for reducing the amount of allogeneic blood transfusion in elective aortic dissection surgery,which is of great significance for saving blood resources and blood protection.

Abstract:Objective Given the alarming increase in the detection rate of suspected scoliosis among teenagers in China with grade,this study aims to evaluate the satisfaction and actual demand for spinal health science popularization education in schools.Methods From October 2024 to June 2025,a cross-sectional survey was conducted among students from 10 primary and secondary schools who participated in the spinal health science popularization activities organized by the spinal health center of Shenzhen Second People's Hospital.A total of 600 questionnaires were distributed,and 582 valid questionnaires were retrieved(response rate:97.00%),and the data were analyzed from the aspects of basic information,satisfaction and acceptance of science popularization lectures,preferences for forms and content demands,behavioral intentions,and multiple logistic regression.Results The study found that the overall acceptance and satisfaction with spinal health science popularization education were high(92.10%"very welcome",88.32%"very understandable").Regarding the preference for lecture forms,there were significant statistical differences among students of different grades(P<0.001).83.19%of primary school students preferred more intuitive and interesting forms such as"interactive games"and"video teaching",while 76.54%of middle and high school students preferred more interactive and analytical forms such as"group discussions"and"case analyses".89.00%of the students expressed that they were"very willing"to share the knowledge they learned about spinal health with their families or friends,Among them,the proportion of primary school students was 92.33%,significantly higher than that of junior and senior high school students,which was 84.36%(P<0.01).86.77%of the students indicated that they paid more attention to their spinal health after participating in this science popularization activity.The results of multiple logistic regression analysis showed that the understandability of the content,the interactivity of the form,and the grade level were independent influencing factors for students'willingness to share knowledge(P<0.05).Conclusion This study confirmed that the grade-based spinal health science popularization strategy is highly feasible and effective.Future science popularization efforts should continue to promote differentiated education based on student needs,enhance the interactivity and practicality of the content,and build a more precise and efficient spinal health education system.

Abstract:Objective To explore the medication patterns of Professor Leng Jinhong in treating thyroid nodules(TN)based on real-world deta.Methods The medical records of patients treated by Professor Leng Jinhong at the expert outpatient department of Liaoning University of Traditional Chinese Medicine from January 2022 to October 2024 were used.According to the inclusion and exclusion criteria,306 cases were included.Excel,IBM SPSS 26.0,and SPSS Modeler 18.0 were used for statistical analysis.Results A total of 121 traditional Chinese medicines were involved.The top six frequently used drugs were Poria cocos,Bupleurum,Oyster shell,Honey-fried Glycyrrhiza,Atractylodes,and Peony bark.From the perspective of drug properties and flarors,cold-natured drugs were used most frequently among the four natures,and sweet-tasting drugs were used most frequently among the five flavors.Cluster analysis identified five groups of drug combinations,and the association rule results showed that Poria cocos-Bupleurum was the most frequent drug pair and had the highest confidence level,indicating a significant synergistic effect in the treatment of TN.Conclusion The medication patterns of Professor Leng in treating TN can be summarized as being guided by the"One Qi Circulation"theory of Huang Yuanyu,with the treatment approach focusing on"regulating the liver as the main point and simultaneously regulating the liver,spleen,and kidney."This study further substantiates the scientific connotation of the"Qi and Water Co-treatment"Poria cocos-Bupleurum drug pair.

Abstract:Objective An experimental study was conducted on the regulation of hypoxia/reoxygenation-induced angiogenesis in human brain microvascular endothelial cells by long non-coding RNA(LncRNA)-maternally expressed gene 3(MEG3)through the microRNA-141-3p/Notch homolog 2(Notch2)axis,to explore whether MEG3 affects the angiogenic capacity of hypoxia/reoxygenation human brain microvascular endothelial cells through the miRNA-141-3p/Notch2 axis.Methods An oxygen-glucose deprivation/reoxygenation(OGD/R)model was established to simulate the in vitro environment of ischemic stroke.Cell viability was detected by the Cell Counting Kit-8(CCK-8)assay,apoptosis was analyzed by flow cytometry,and the expression levels of genes and proteins were detected by real-time fluorescence quantitative polymerase chain reaction(RT-qPCR)and Western blot,respectively.Cell migration and angiogenic ability were evaluated by Transwell and tube formation assays,respectively.The interaction between MEG3,poly-pyrimidine tract-binding protein 1(PTBP1),and Notch2 was analyzed by techniques such as RNA immunoprecipitation(RIP),RNA pull-down,and fluorescence in situ hybridization(FISH).In addition,a mouse middle cerebral artery occlusion(MCAO)model was established for in vivo validation.Results OGD/R treatment significantly upregulated the expression level of MEG3 in HBMECs,accompanied by decreased cell viability,increased apoptosis,and weakened migration and angiogenic ability.Knockdown of MEG3 reversed the above OGD/R-induced cell damage.Mechanistically,MEG3 was mainly located in the cytoplasm and directly bound to RNA-binding protein PTBP1,further regulating the stability of Notch2 messenger RNA(mRNA).Under OGD/R conditions,MEG3 enhanced Notch2 expression through PTBP1,thereby inhibiting the migration and angiogenesis of HBMECs.In vivo experiments further confirmed that silencing MEG3 significantly reduced the infarct area,improved neurological function scores,and reduced apoptosis.Conclusion MEG3 enhances the mRNA stability of Notch2 by binding to PTBP1,thereby inhibiting the migration and angiogenesis of HBMECs and promoting the development of ischemic stroke.Targeting the MEG3/PTBP1/Notch2 axis may provide a new potential target for the treatment of ischemic stroke.