Abstract:Objective To analyze the effect of transcranial magnetic stimulation(TMS)combined with language training on language development delay in children.Methods A total of 102 children with delayed language development admitted to the Third Affiliated Hospital of Luohe Medical College from February 2023 to February 2025 were selected as the research subjects.They were divided into a control group(receiving language training)and an observation group(receiving TMS combined with language training)according to the random number table method,with 51 cases in each group.The therapeutic effects of the two groups of children were compared,including language development quotient,oral motor ability,comprehension,expression and executive ability before and after treatment.Results The total effective rate of treatment in the observation group was higher than that in the control group,and the difference was statistically significant(P<0.05).After 1 month,2 months and 3 months of treatment,the scores of the Gesell neuropsychological development scale for children in the observation group were all higher than those in the control group,and the differences were statistically significant(P<0.05).After three months of treatment,the scores of the scores of each dimension of the simple oral movement scale of the children in the observation group were higher than those in the control group,and the differences were statistically significant(P<0.05).After three months of treatment,the comprehension,expression and executive abilities of the children in the observation group were all higher than those in the control group,and the differences were statistically significant(P<0.05).Conclusion The combination of TMS on the basis of language training can significantly improve the language,oral motor ability,comprehension,expression and executive ability of children,and further enhance the therapeutic effect.

Abstract:The occurrence of colorectal cancer(CRC)is closely related to the imbalance of gut microbiota.The author of this article,based on the theory of"evil Elimination and righteousness restoration",analogizes the dynamic balance between probiotics and pathogenic bacteria as the microscopic manifestation of"the struggle between evil and righteousness".Pathogenic bacteria accelerate carcinogenesis through pro-inflammatory microenvironments,while probiotics inhibit tumors through immune regulation and other mechanisms.Traditional Chinese Medicine can restore the balance of the microbiota through the synergy of multiple components.Traditional Chinese Medicine formulas can inhibit the carcinogenic pathways of the microbiota,such as reducing the proliferation of pathogenic bacteria,promoting the colonization of probiotics,and repairing the intestinal barrier.The author of this article focuses on demonstrating that Traditional Chinese Medicine and its formulas do not simply act on a single strain but follow the therapeutic principle of"supporting righteousness and eliminating evil",regulating the structure and function of the microbiota through multiple components and multiple targets,and restoring the intestinal microecological homeostasis,which to a certain extent promotes the application of integrated Traditional Chinese and Western Medicine in the precise intervention of CRC.

Abstract:Objective Evaluation of the core knowledge mastery level of tuberculosis patients in treatment and its influencing factors.Methods A cross-sectional survey design was adopted.From January 2025 to July 2025,a questionnaire survey on the core knowledge awareness rate of tuberculosis was conducted on 184 in-patients with active tuberculosis diagnosed at the first outpatient department of a tuberculosis specialty hospital in Guangzhou.The questionnaire covered basic information,five core knowledge points of tuberculosis,health education experience,and the main channels for obtaining knowledge about tuberculosis.A total of 184 valid questionnaires were recovered.The differences in characteristics among patients with different levels of core knowledge mastery were analyzed,and a binary Logistic regression model was constructed with core knowledge mastery being the dependent variable to analyze potential influencing factors.Results Among the 184 in-patients with active tuberculosis,the overall core knowledge awareness rate was 77.61%(95%CI:0.749-0.803).The single knowledge mastery rates of the five core knowledge points were 82.07%,89.13%,41.85%,85.33%,and 89.67%respectively.Only 29.35%were all correct.The differences in the awareness rates of each item were statistically significant(P<0.01).In the univariate analysis of the awareness situation of tuberculosis core knowledge among in-patients with active tuberculosis,the core knowledge qualification rate of patients who received health education was significantly higher than that of those who did not(χ2=7.593,P=0.006).Among the different information acquisition channels,"doctor's explanation"was the main source of health education information,accounting for 46.70%.Those who obtained knowledge mainly through"doctor's explanation"had the highest core knowledge mastery qualification rate.The binary Logistic regression analysis further confirmed that receiving health education(OR=0.435,95%CI:0.183-1.034)and"doctor's explanation"as the main channel(OR=10.507,95%CI:1.833-60.233)were promoting factors for core knowledge mastery.Conclusion Under the strong and effective pulmonary tuberculosis prevention and control strategies and health promotion measures,the core knowledge level of patients under treatment for pulmonary tuberculosis has significantly improved compared to previous levels.However,it still falls short of the target of 85%awareness rate of core knowledge among the public set in the"National Tuberculosis Prevention and Control Plan(2024-2030)".It is recommended to further strengthen health education for patients under treatment for pulmonary tuberculosis,give full play to the role and professional advantages of medical staff,innovate publicity forms and concepts,empower new methods for tuberculosis health education,and continuously inject new impetus into tuberculosis prevention and control.

Abstract:Objective To explore the effect of modified Xiaochengqi Decoction combined with conventional Western medicine on the recovery time of gastrointestinal function and gastrointestinal hormones in patients with postoperative abdominal distension after cesarean section.Methods A total of 86 patients with postoperative abdominal distension after cesarean section admitted to Zhumadian Hospital of Tiaditional Chinese Medicine Affiltiated to Henan University of Chinese Medicine from February 2023 to February 2025 were selected as the research subjects and randomly divided into a control group and an observation group,with 43 cases in each group.The control group received conventional Western medicine treatment,while the observation group was treated with modified Xiaochengqi Decoction in addition to the treatment of the control group.The recovery time of gastrointestinal function,pain degree,traditional Chinese medicine(TCM)syndrome score,gastrointestinal hormones,quality of life before and after treatment and adverse reactions were compared between the two groups.Results The time for relief of gastrointestinal discomfort,recovery of bowel sounds,first defecation and disappearance of abdominal distension in the observation group was shorter than that in the control group,and the difference was statistically significant(P<0.05).After treatment,the scores of abdominal distension,nausea and vomiting,visual analogue scale(VAS)score,and the level of serum vasoactive intestinal peptide(VIP)level in the observation group were lower than those in the control group,while the levels of serum gastrin(GAS)and motilin(MTL)and the scores of each dimension of the generic quality of life inventory-74(GQOLI-74)in the observation group were higher than those in the control group,and the differences were statistically significant(P<0.05).There was no statistically significant difference in the total incidence of adverse reactions between the two groups(P=0.333).Conclusion The combination of modified Xiaochengqi Decoction and conventional Western medicine in the treatment of postoperative abdominal distension after cesarean section can accelerate the recovery of gastrointestinal function,relieve clinical symptoms,reduce the degree of abdominal pain,regulate the levels of gastrointestinal hormones,and improve the quality of life without increasing adverse reactions.

Abstract:Objective To explore the impact of the solution-focused nursing model based on structure and psychology on blood glucose fluctuations,diabetes distress scores,and quality of life in elderly patients with type 2 diabetes mellitus(T2DM).Methods A total of 204 elderly patients with T2DM admitted to the Second Affiliated Hospital of Zhengzhou University from January 2024 to March 2025 were selected as the research subjects.They were divided into a control group and an experimental group according to the random number table method,with 102 cases in each group.Patients in the control group received conventional nuesing intervention,while patients in the experimental group received the solution-focused nursing model based on structure and psychology+conventional nursing intervention.The blood glucose fluctuations,psychological distress,self-efficacy,self-management behaviors,quality of life and nursing satisfaction of the two groups of patients before and after the intervention were compared.Results After the intervention,the coefficient of variation(CV),mean amplitude of glycemic excursion(MAGE),largest amplitude of glycemic excursion(LAGE),and the scores of each dimension of the diabetes distress scale of the patients in the experimental group were all lower than those in the control group,and the differences were statistically significant(P<0.05).After the intervention,the scores of each dimension of the diabetes management self-efficacy scale(DMSES),the diabetes summary of diabetes self care activities(SDSCA)were all higher than those in the control group,and diabetes specific qualify of life the diabetes specific quality of life(DSQL)in the experimental group were all lower than those in the control group,and the differences were statistically significant(P<0.05).The total satisfaction rate of nursing in the experimental group was higher than that in the control group,and the difference was statistically significant(P<0.05).Conclusion The application of the solution-focused nursing model based structure and psychology in elderly patients with T2DM,it can effectively slow down blood glucose fluctuations,relieve psychological distress,enhance self-efficacy,improve self-management behaviors,and increase the quality of life and nursing satisfaction.

Abstract:Objective To analyze the clinical effect of the combination of Tiaodu LiJin acupuncture with acupoint embedding thread in the treatment of lumbar disc herniation(LDH)based on the changes in lumbar spine range of motion,muscle strength and pain-related factors.Methods A total of 102 LDH patients admitted to Huzhen Central Health Center of Longyou County from June 2022 to May 2024 were selected as the research subjects and randomly divided into celecoxib group and combined treatment group,with 51 cases in each group.Patients in the celecoxib group were treated with oral celecoxib capsules,while patients in the combined treatment group were treated with Tiaodu LiJin acupuncture combined with acupoint embedding thread on the basis of oral celecoxib capsules.The clinical efficacy,lumbar spine range of motion,lumbar muscle strength,pain-related factors and treatment safety of the two groups before and after treatment were compared.Results The total effective rate of the combined treatment group was higher than that of the celecoxib group,and the difference was statistically significant(P<0.05).After treatment,the lumbar flexion,extension,and ipsilateral lateral flexion range of motion,as well as the lumbar flexion,extension,rotation and lateral flexion muscle strength of the combined treatment group were greater than those of the celecoxib group,and the differences were statistically significant(P<0.05).After treatment,the levels of serum 5-hydroxytryptamine(5-HT),prostaglandin E2(PGE2),substance P(SP),and neuropeptide Y(NPY)in the combined treatment group were lower than those in the celecoxib group,and the differences were statistically significant(P<0.05).There was no statistically significant difference in the total incidence of adverse reactions between the two groups(P>0.05).Conclusion The combination of Tiaodu LiJin acupuncture with acupoint embedding thread in the treatment of LDH has definite efficacy,can improve the lumbar spine range of motion and muscle strength,regulate the levels of pain-related factors,and has good safety.

Abstract:Objective To compare the effects of transmission orthodontics and traditional straight wire arch orthodontics in treating skeletal Class Ⅲ malocclusion in adolescents during the permanent dentition period.Methods Sixty adolescent patients with skeletal Class Ⅲ malocclusion who were admitted to Zhengzhou Zhongnuo Stomatological Hospital from January 2022 to May 2023 during the permanent dentition period were selected as the research subjects for a retrospective study.According to the treatment methods,they were divided into a traditional straight wire arch group(27 cases,who received traditional straight wire arch correction)and a transmission straight wire arch group(33 cases,who received transmission orthodontics treatment).Propensity score matching was used to correct the differences between groups.The matching variables included age,gender,severity of bony disease[a point-hasion-b point angle(ANB Angle)],etc.After matching,the baseline characteristics of the two groups of patients were balanced.All patients were continuously observed for 24 months,and the complete follow-up rate was 100%.The therapeutic effects of the two groups of patients,the lip inclination of the maxillary and mandibular central incisors before and after orthodontic treatment[the lower internal angle between the long axis of the maxillary central incisor and the sella-nasion plane(U1-SN angle),the upper internal angle between the long axis of the mandibular central incisor and the mandibular plane(L1-MP angle)],and the satisfaction with the improvement of facial profile were compared.Results The overall clinical efficacy of patients in the transmission straight wire arch group was better than that in the traditional straight wire arch group,and the difference was statistically significant(P<0.05).Twelve months after orthodontic treatment,the U1-SN Angle in the transmission straight wire archway group was smaller than that in the traditional straight wire arch group,and the L1-MP Angle was larger than that in the traditional straight wire arch group.The differences were statistically significant(P<0.05).The total satisfaction rate of patients in the transmission straight wire arch group was higher than that in the traditional straight wire arch group,and the difference was statistically significant(P<0.05).Conclusion Compared with the traditional straight wire arch orthodontic technique,transmission orthodontic treatment can achieve more significant bony improvement effects in intervening in skeletal Class Ⅲ malocclusion in adolescents during the permanent dentition period.At the same time,the compensatory inclination degree of the upper and lower central incisors is smaller,and patients'satisfaction with the orthodontic posterior facial shape is also higher.

Abstract:Objective To analyze the expression differences of D-dimer(D-D)and four coagulation items in pregnant women of different gestational periods and explore their clinical value.Methods The clinical data of 89 pregnant women in Suiyang District Maternal and Child Health Hospital of Shangqiu City from January 2022 to December 2024 were retrospectively collected.The levels of D-D and four coagulation items[prothrombin time(PT),activated partial thromboplastin time(APTT),thrombin time(TT),fibrinogen(FIB)]of pregnant women in different pregnancy periods were compared.According to the pregnancy outcomes,they were divided into a poor group(9 cases)and a good group(80 cases).The levels of D-D and four coagulation items at different gestational periods of pregnant women with different pregnancy outcomes were compared,and the receiver operating characteristic(ROC)curve was drawn to explore its predictive value.Results The comparison of D-D and FIB levels of pregnant women at different gestational periods,from high to low,was in the labor period,late pregnancy,the mid-pregnancy,and the early pregnancy,and the differences were statistically significant(P<0.05).The comparison of PT,APTT and TT,from long to short,was in the early pregnancy,the mid-pregnancy,the late pregnancy and the period of labor,and the differences were statistically significant(P<0.05).The levels of D-D and FIB in the poor group of pregnant women at each pregnancy were higher than those in the good group,PT,APTT and TT were all shorter than those in the good group,and the differences were statistically significant(P<0.05).The ROC curve shows that D-D,PT,APTT,TT,and FIB at different gestational periods all have good predictive values for poor pregnancy outcomes in pregnant women,and the combined predictive value is even higher.However,there was no statistically significant difference in the combined predictive value among early,middle,and late pregnancy and labor(P>0.05).Conclusion With the increase of pregnancy,the levels of D-D and FIB in pregnant women rise,while PT,APTT,and TT shorten.Moreover,D-D,PT,APTT,TT,and FIB in different pregnancy periods all have good predictive values for poor pregnancy outcomes in pregnant women.

Abstract:Objective To compare the therapeutic effects of Xiebai Powder combined with meridian massage and Xiebai Powder alone in the treatment of respiratory syncytial virus pneumonia(phlegm-heat and lung closure type)in children,and to provide new ideas and methods for the diagnosis and treatment of this disease.Methods Eighty children with respiratory syncytial virus pneumonia of phlegm-heat and lung closure type admitted to Zhengzhou Traditional Chinese Medicine Hospital from September 2024 to March 2025 were selected as the research objects.They were divided into a treatment group and a control group according to the random number table method,with 40 cases in each group.After admission,both groups of children received basic treatments including antiviral therapy,antiasthmatic therapy and relief of mucosal edema.On this basis,the children in the control group were treated with Xiebai Powder,while those in the treatment group were treated with Xiebai Powder combined with meridian-following massage.The therapeutic effects,disappearance time of symptoms and signs,time for respiratory syncytial virus to turn negative,and traditional Chinese medicine(TCM)syndrome scores before and after treatment of the two groups of children were compared.Results The total effective rate of treatment for both groups of children was 100.00%,but the clinical cure rate in the treatment group was higher than that in the control group,and the difference was statistically significant(P<0.05).The disappearance time of cough,wheezing,fever,throat congestion with wheezing and pulmonary rales in the treatment group was shorter than that in the control group,and the difference was statistically significant(P<0.05).The time for respiratory syncytial virus to turn negative in the treatment group was(6.72±1.34)days,which was shorter than that in the control group[(7.58±1.66)days],and the difference was statistically significant(P<0.05).After the treatment,the TCM syndrome score of the children in the treatment group was lower than that in the control group,and the difference was statistically significant(P<0.05).Conclusion Xiebai Powder combined with meridian-following massage has a remarkable therapeutic effect on respiratory syncytial virus pneumonia(phlegm-heat and lung closure type)in children.It can also improve clinical symptoms of the children and shorten the time for the virus to turn negative.

Abstract:Objective To explore the mechanism by which palbociclib reverses doxorubicin-resistant human breast cancer(BC)cell line MCF7/ADR.Methods Different concentrations of palbociclib were added to the bc doxorubicin-resistant cell line MCF7/ADR cells for treatment to observe the effects of palbociclib on the proliferation and invasion of the bc doxorubicin-resistant cell line MCF7/ADR cells.The possible mechanism by which palbociclib inhibits the proliferation of doxorubicin-resistant cell lines MCF7/ADR cells was detected and verified by transcriptome sequencing(RNA-seq).Results The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay verified that the effective half-maximal inhibitory concentration(IC50)concentration of palbociclib for inhibiting the proliferation of the bc doxorubicin-resistant cell line MCF7/ADR was 3.8 μM.MTT,wound healing,Transwell,and 5-ethynyl-2'-deoxyuridine(EdU)assays collectively demonstrated that palbociclib can suppress its proliferation and invasion.Flow cytometry experiments confirmed that palbociclib could promote its apoptosis and inhibit its cell cycle at the G1 phase.Rna-seq assay revealed that palbociclib inhibits the proliferation of bc doxorubicin-resistant cell lines MCF7/ADR cells mainly by regulating the cell cycle and simultaneously inhibiting the cyclin E1(CCNE1).Overexpression of CCNE1 could partially reverse the proliferation and invasion ability of palbociclib against bc doxorubicin-resistant cell lines MCF7/ADR cells.Conclusion Palbociclib may affect the proliferation and invasion ability of BC doxorubicin-resistant cell line MCF7/ADR by regulating the expression of CCNE1.

Abstract:Objective To analyze the causes and risk prevention of adverse delivery outcomes in pregnant women in medical malpractice cases,and to provide theoretical support and practical guidance for clinical perinatal risk prevention and maternal and infant safety assurance.Methods Retrospective analysis was conducted on 46 cases of medical malpractice cases involving adverse delivery outcomes in pregnant women and where medical negligence was determined,which were received by Guangdong Zhongyi Forensic Science Center from January 2019 to December 2024.The overall distribution of medical negligence was clarified,and the correlation between hospital grades and main types of negligence was analyzed.Based on the analysis results,risk prevention measures for adverse delivery outcomes after delivery were proposed.Results From the distribution of medical negligence by hospital grades,13 cases(28.3%)involved secondary hospitals and 33 cases(71.7%)involved tertiary hospitals;from the distribution of medical negligence by process,medical negligence mainly occurred in prenatal assessment and testing,during delivery,neonatal care,doctor-patient communication and medical record writing.Among them,the negligence during delivery was the most prominent,with 17 cases,accounting for 37.0%,and 12 of these cases(26.1%)were caused by"delay in decision to transfer to cesarean section";the negligence in the neonatal care process was the second,with 15 cases,accounting for 32.6%,and 9 of these cases(30.8%)were caused by the non-standardization of the neonatal resuscitation process(NRP).The analysis of the correlation between hospital grades and main types of negligence showed that among the 12 cases with delayed decision to transfer to cesarean section,10 cases(30.3%)occurred in tertiary hospitals and 2 cases(15.4%)occurred in secondary hospitals;among the 9 cases with non-standardization of NRP,5 cases(15.2%)occurred in tertiary hospitals and 4 cases(30.8%)occurred in secondary hospitals.The Fisher exact test showed that although the incidence of NRP non-standardization in secondary hospitals was higher than that in tertiary hospitals,and the incidence of delayed decision to transfer to cesarean section in tertiary hospitals was higher than that in secondary hospitals,the differences were not statistically significant(P>0.05).Conclusion The occurrence of perinatal medical malpractice is closely related to the standardization of all-round diagnosis and treatment behaviors throughout the process of prenatal,during delivery,and post-delivery.Establishing a full-cycle risk early warning mechanism,standardizing diagnosis and treatment operation procedures,and improving the emergency response capabilities of medical staff are key measures.

Abstract:Objective To observe the efficacy and safety of programmed death receptor-1(PD-1)inhibitor combined with oxaliplatin+capecitabine(CAPEOX)regimen in neoadjuvant therapy for advanced gastric cancer patients.Methods The clinical data of 80 advanced gastric cancer patients admitted to the First Affiliated Hospital of Guangxi Medical University from January 2023 to December 2024 were retrospectively collected.According to the neoadjuvant treatment regimens,they were divided into a control group(40 cases,CAPEOX regimen)and an observation group(40 cases,PD-1 inhibitor combined with CAPEOX regimen).The clinical efficacy,pathological remission,levels of serum tumor markers carbohydrate antigen(CA)125,CA199,carcinoembryonic antigen(CEA),CA724 before and after treatment,and the occurrence of adverse reactions were compared between the two groups.Results The objective response rate(ORR)and pathological complete response(pCR)rate in the observation group were higher than those in the control group,with statistically significant differences(P<0.05).After treatment,the levels of serum CA125,CA199,CEA,and CA724 in both groups were lower than those before treatment,and the levels in the observation group were lower than those in the control group,with statistically significant differences(P<0.05).There was no statistically significant difference in the degrees of leukopenia,neutropenia,thrombocytopenia,diarrhea,nausea and vomiting,peripheral neurotoxicity,liver and kidney function damage,immune-related pneumonia,hand-foot syndrome,and thyroid function abnormalities between the two groups(P>0.05).Conclusion The neoadjuvant therapy of PD-1 inhibitor combined with CAPEOX for advanced gastric cancer patients is effective,can effectively reduce the levels of serum tumor markers,and does not increase the risk of adverse reactions.

Abstract:Objective To explore the related influencing factors of venous thromboembolism(VTE)in patients after gastrointestinal surgery and propose targeted preventive strategies.Methods A retrospective analysis was conducted on the clinical data of 120 patients who underwent gastrointestinal surgery in Jian'ou Municipal Hospital from June 2024 to June 2025.The patients were divided into a VTE group(26 cases)and a non-VTE group(94 cases)based on whether VTE occurred within 14 days after surgery.General patient information,surgical-related indicators,laboratory indicators,and postoperative care indicators were collected.Univariate analysis was first used to screen the factors that might affect the occurrence of VTE after surgery,and then multivariate Logistic regression analysis was performed on the factors with statistical significance to identify the independent risk factors for VTE after surgery.Results Among the 120 patients who underwent gastrointestinal surgery,26 developed VTE within 14 days after surgery,with an incidence rate of 21.67%.Univariate analysis showed that there were statistically significant differences in age≥60 years,diabetes mellitus,operation duration≥3 hours,preoperative D-dimer level>0.5 mg·L-1,and postoperative bed rest time≥72 hours between the two groups(P<0.05).Logistic regression analysis results indicated that age≥60 years(OR=5.259,95%CI:2.002～13.816),diabetes mellitus(OR=2.750,95%CI:1.131～6.688),operation duration≥3 hours(OR=7.471,95%CI:2.716～20.551),postoperative bed rest time≥72 hours(OR=14.600,95%CI:4.912～43.398),and preoperative D-dimer level>0.5 mg·L-1(OR=14.385,95%CI:4.522～45.760)were independent risk factors for VTE after gastrointestinal surgery(P<0.05 and OR>1).Conclusion The occurrence of VTE after gastrointestinal surgery is closely related to age,history of diabetes,operation duration,postoperative bed rest time,and preoperative D-dimer level.Clinical individualized preventive strategies should be formulated based on these risk factors to reduce the risk of VTE after surgery.

Abstract:Objective To explore the predictive value of serum soluble growth stimulation expressed gene 2 protein(sST2)level for sepsis-associated acute kidney injury(SA-AKI).Methods Seventeen patients with SA-AKI admitted to the People's Hospital of Longhua,Shenzhen from February 2023 to February 2025 were selected as the study subjects(SA-AKI group),and 17 patients with simple sepsis were selected as the control group.The basic clinical data and laboratory test results of the two groups of patients within 24 hours of admission were collected,and the serum sST2 levels of the two groups of patients were compared.The correlation between serum sST2 levels and sequential organ failure assessment score(SOFA)and acute physiology and chronic health evaluation score(APACHE II)was analyzed.By drawing the receiver operating characteristic(ROC)curve,the area under the curve(AUC)of serum sST2 levels and related laboratory indicators was compared,and the predictive value of serum sST2 levels for SA-AKI was analyzed.Results The serum sST2 level of the SA-AKI group was significantly higher than that of the control group,and the difference was statistically significant(P<0.05);The increase in serum sST2 level was positively correlated with SOFA and APACHE II scores.The R2 values of the scatter plots were 0.574 and 0.676,respectively.The ROC curve was drawn,and the AUC(95%CI)of serum sST2 level for predicting SA-AKI was 0.871(0.752-0.991),which was higher than other laboratory indicators,and the difference was statistically significant(P<0.05).Conclusion The serum sST2 level of SA-AKI patients was significantly increased,and the serum sST2 level has a certain predictive value for SA-AKI.

Abstract:Objective To explore the distribution characteristics of pathogens causing upper respiratory tract infections in children in Xiamen in different age groups,genders,and seasons,and to provide references for disease prevention,diagnosis,and treatment.Methods A retrospective study was conducted on the nucleic acid test results of six upper respiratory tract pathogens in 7911 children who visited the First Affiliated Hospital of Xiamen University from September 2023 to August 2024.The infection rates of these six pathogens in children of different age groups,genders,and seasons were analyzed.Results Among the 7911 children tested,1568 children showed positive for one or more of the six pathogens,with a positive rate of 19.82%.The proportions of children infected with adenovirus(ADV),human rhinovirus(HRV),and Mycoplasma pneumoniae(MP),influenza virus A(FluA),influenza virus B(FluB),respiratory syncytial virus(RSV),were 12.44%,24.74%,and 43.36%,14.22%,13.13%,3.65%,respectively.There were statistically significant differences in the positive detection rates of the six pathogens among different age groups(P<0.05).There was no statistically significant difference in the positive detection rates of the six pathogens between different genders(P>0.05).There were statistically significant differences in the positive rates of ADV,MP,FluA,FluB,and RSV among different seasons(P<0.05).Among them,the positive detection rates of the six pathogens in different seasons were as follows:ADV peaked in spring and summer,MP had a higher detection rate in autumn and winter,the epidemic periods of FluA and FluB were in winter,and the peak of RSV was in spring.Conclusion The positive detection rates of the six pathogens in children with upper respiratory tract infections are relatively high,and their infection rates are related to the children's age,gender,and season.Families,schools,communities,and medical institutions should take appropriate prevention and control measures based on the transmission patterns of these pathogens to ensure accurate diagnosis and precise treatment,reduce the inappropriate use of antibiotics,and alleviate the burden on families.

Abstract:Objective To evaluate the feasibility of preparing three types of blood products from insufficient whole blood.Methods A total of 98 bags of insufficient whole blood ranging from 180 to 269 mL collected by Dongguan Central Blood Station from February 2023 to February 2024 were randomly selected and divided into two groups.Among them,a group collected 221 to 269 mL of insufficient whole blood and first prepared it into whole blood that met the corresponding specification standards.Another group collected 180 to 220 mL of whole blood.A total of 98 1 U bags of leukocyte-reduced suspended red blood cells,60 1 U bags of fresh frozen plasma,and 38 1 U bags of cryoprecipitated coagulation factors were prepared in the two groups.All blood varieties were divided into two groups.The blood products prepared by removing the maintenance fluid and part of the whole blood with a collection volume of 221-269 mL of whole blood were classified as group 1,and the blood varieties directly prepared by collecting 180-220 mL of whole blood were classified as group 2.The quality inspection results of the three blood products were analyzed and compared.Results The volume and hemoglobin(Hb)content of group 2 were lower than those of group 1,but the qualified rate of volume was higher than that of group 1,and the differences were statistically significant(P<0.05).There was no statistically significant difference in the volume of fresh frozen plasma,plasma protein content,factor Ⅷ content and the qualification rate of each index between the two groups(P>0.05).There was no statistically significant difference in the volume of cold precipitation coagulation factors,fibrinogen content,factor Ⅷ content and the qualified rates of each index between the two groups(P>0.05).Conclusion It is feasible to prepare leukocyte-reduced suspended red blood cells and cryoprecipitate coagulation factors using insufficient whole blood,which can effectively reduce blood waste.However,during the collection and preparation process,certain measures should also be taken to unify the preparation standards of insufficient blood,thereby improving the quality of blood products.It provides a new focus and scientific reference basis for improving the quality control of insufficient blood products.

Abstract:Objective To conduct a visual analysis of domestic research on prescription drugs using bibliometric methods,revealing the research hotspots and development trends in the field of prescription drugs from 2000 to 2025.Methods Relevant research literature on prescription drugs included in the China National Knowledge Infrastructure(CNKI)from January 1,2000 to May 1,2025 was retrieved.Author collaboration analysis,institutional collaboration network construction,keyword co-occurrence mining,cluster analysis,timeline analysis,and burst detection were conducted using CiteSpace 6.2.R5 software to comprehensively analyze the research progress of prescription drugs.Results A total of 810 Chinese-language articles were included after screening.The number of publications fluctuated in phases due to policy and technological influences.A collaborative network of institutions was formed with China Pharmaceutical University and the National Medical Products Administration at the core,but both author and institutional collaborations were relatively loose.Research hotspots focused on the classified management of prescription drugs,risk prevention and control in the circulation field,and innovative regulatory models.The evolution of keywords reflected three-stage characteristics:from basic management to risk prevention and control,and then to the innovation of"Internet+prescription drugs".Conclusion Future research on prescription drugs will strengthen the application of interdisciplinary methods,focusing on directions such as artificial intelligence-enabled smart supervision,optimization of the"Internet+prescription drugs"model,and blockchain technology for traceability,promoting research towards intelligence and precision.

Abstract:Objective To analyze the influencing factors of natural pregnancy failure after laparoscopic surgery in patients with ovarian endometriosis cysts and construct a prediction model.Methods A total of 246 patients with ovarian cysts admitted to Nanyang Central Hospital from April 2022 to April 2024 were selected as the research subjects.They were followed up for one year after surgery and divided into a failure group(95 cases)and a success group based on whether they achieved natural pregnancy.Univariate and multivariate analyses were conducted to identify the independent risk factors for natural pregnancy failure after laparoscopic surgery and construct a prediction model.Results There were statistically significant differences in age,infertility type,cyst diameter,revised American Fertility Society(r-AFS)stage,the number of uterine cavity operations,endometriosis fertility index(EFI)score,and the treatment situation with gonadotropin-releasing hormone agonists between the two groups(P<0.05).Logistic multivariate analysis showed that age≥35 years,primary infertility,cyst diameter 3-5 cm or>5 cm,r-AFS stage Ⅲ-Ⅳ,uterine cavity operation≥2 times,EFI score≤4 points,and no postoperative use of GnRH-a treatment were independent risk factors for natural pregnancy failure after laparoscopic surgery(P<0.05).Based on the results of multivariate analysis,the prediction model for natural pregnancy failure after laparoscopic surgery was fitted as Logit(P)=-6.694+0.680×age+0.882×primary infertility+0.822×cyst diameter+0.740×r-AFS stage+0.627×uterine cavity operation+0.655×EFI score+0.836×no postoperative use of GnRH-a treatment.Receiver operating characteristic(ROC)curve analysis showed that the area under the curve for predicting natural pregnancy failure after laparoscopic surgery was 0.932,with a sensitivity of 0.924,and a specificity of 0.818.Conclusion Patients with age≥35 years,primary infertility,cyst diameter(3-5 cm,>5 cm),r-AFS stage Ⅲ-Ⅳ,uterine cavity operation≥2 times,EFI score≤4 points,and no postoperative use of GnRH-a treatment are more likely to experience natural pregnancy failure.The prediction model established by combining these factors can identify high-risk groups for natural pregnancy failure after surgery.

Abstract:Objective To reveal the research landscape,evolution of research hotspots,and differences between Chinese and foreign studies on recurrent respiratory tract infections(RRTI)in children,thereby providing a reference for the precise positioning and improvement of related research in this field in China.Methods The data for this study were sourced from the WoS core collection,with the search period from January 1,2015 to March 1,2025.The search term"TS=(recurrent respiratory tract infection* OR RRTI)AND TS=(child* OR pediatric* OR infant*)AND TS=(treat* OR therapy OR management or intervention)"was used to extract the literature.After the initial acquisition of the literature,duplicate removal was conducted using"EndNote"software,followed by manual screening,ultimately retaining a total of 384 English research articles and reviews that met the requirements.Tools such as"CiteSpace 6.4.2R","VOSviewer 1.6.19"and"Excel 2019"were utilized to conduct a systematic analysis of the annual publication volume,country/region,institution,author,journal distribution,and characteristics of keywords and emerging words.Results The global average annual publication volume was approximately 38.4 articles.The overall research trend can be summarized into three stages:"fluctuation rise-peak-adjustment".The peak was reached in 2022(51 articles),and there was a decline after 2023 due to the reallocation of resources caused by the global coronavirus disease 2019(COVID-19)pandemic.In terms of national contributions,the United States(106 articles,with an average citation of 26.5 times per article)and China(56 articles,with an average citation of 8.3 times per article)were the main research-producing countries.However,the proportion of highly cited papers from China was only 8.9%,indicating that there is still room for improvement in research influence.At the institutional level,two typical research models emerged:one was"scale-type output",such as the University of Melbourne(10 articles,with an average citation of 19.6 times per article);the other was"quality-type breakthrough",such as the University of Oxford(only 5 articles,but with an average citation of 258 times per article).Keyword clustering analysis identified three core hotspots:immune regulation therapy,intestinal microecology,and rational use of antibiotics.Among them,"impact"had the highest burst intensity from 2020 to 2022(4.13),reflecting the extensive attention paid to the impact on clinical practice during this period.Conclusion Global research on pediatric RRTI has formed a relatively stable collaborative network,yet there are significant disparities in research quality across different regions.Although China ranks among the top in terms of publication volume in this field,it still faces a structural imbalance characterized by"emphasis on clinical observation over mechanistic exploration."To further enhance research impact,future efforts should focus more on cutting-edge directions such as"gut microbiome modulation"and"basic-clinical translation,"while promoting an overall leap in research quality through strengthened international collaboration.

Abstract:Objective To explore the nutritional status and influencing factors of patients with endometrial cancer after adjuvant chemotherapy.Methods A total of 104 patients with endometrial cancer who received adjuvant chemotherapy at Nanyang Central Hospital from March 2023 to March 2025 were selected as the research subjects.According to the patient-generated subjective global assessment(PG-SGA)score,they were divided into a good nutritional status group(58 cases,total score<4)and a malnutrition group(46 cases,total score≥4).The general data and nutritional indicators[serum total protein(TP),albumin(ALB),prealbumin(PAB)levels]of the two groups were compared,and Logistic regression analysis was used to analyze the factors influencing the nutritional status of patients with endometrial cancer after adjuvant chemotherapy.Results There were statistically significant differences in age,body mass index(BMI),chemotherapy stage,whether diabetes was combined,whether there were gastrointestinal reactions,coping styles,and the proportion of taste changes between the two groups(P<0.05);the levels of serum TP,ALB,and PAB in the malnutrition group were lower than those in the good nutritional status group,and the differences were statistically significant(P<0.05);multivariate Logistic regression analysis showed that advanced chemotherapy stage,combined diabetes,and negative coping style were independent risk factors affecting the nutritional status of patients with endometrial cancer after adjuvant chemotherapy(P<0.05).Conclusion Advanced chemotherapy stage,combined diabetes,and negative coping style are independent risk factors affecting the nutritional status of patients with endometrial cancer after adjuvant chemotherapy.