Abstract:Objective To explore the prevalence and influencing factors of gout among residents in Chifeng area,Inner Mongolia Autonomous Region.Methods The laboratory data of 1 126 examinees in the Physical Examination Center of Chifeng Songshan Hospital from January to June 2025 were collected,including gender,age,serum uric acid(UA),total cholesterol(TC),triacylglycerol(TG),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C)and blood glucose(GLU).According to whether they had gout or not,they were divided into gout group(124 cases)and control group(1 102 cases),and the differences of above indicators between two groups were compared.There were 78 males and 46 females in gout group,and they were divided into 20-40 years old group(47 cases),41-60 years old group(70 cases)and≥61 years old group(7 cases)according to age.The differences in above indicators between gout group and control group,as well as the level of UA among gout patients of different genders and age groups were analyzed.Results The proportion of males among gout patients was significantly higher than that of females(62.90%vs.37.10%,P<0.05).The proportion of gout patients in 41-60 years old group had the highest proportion(56.45%),and those in≥61 years old group had the lowest proportion(2.98%).Among male gout patients,the level of UA in 41-60 years old group was significantly higher than those in 20-40 years old group and≥61 years old group(mmol/L:532.76±79.54 vs.489.38±71.32,471.51±42.29,both P<0.05).Among female gout patients,the level of UA in 41-60 years old group was higher than that in 20-40 years old group(mmol/L:424.47±58.71 vs.406.33±41.00,409.93±34.75,all P<0.05).The levels of TG and GLU in gout patients were higher than those in control group,and the level of HDL-C was lower than that of control group[TG(mmol/L):2.43±0.53 vs.1.57±0.58;GLU(mmol/L):6.04±1.25 vs.5.07±0.52;HDL-C(mmol/L):1.09±0.16 vs.1.51±0.32;all P<0.05).Conclusions The proportion of male gout patients in Chifeng area is higher than that of female patients.The UA level of gout patients in 41-60 years old group is the highest,and TG may be an important factor causing gout in the population of this area.

Abstract:Objective To explore the predictive value of combined detection of D-dimer,serum amyloid A(SAA)and N-terminal pro-brain natriuretic peptide(NT-proBNP)for chronic obstructive pulmonary disease(COPD)combined with acute lower respiratory tract infection(ALRTI).Methods The 87 patients with COPD admitted to the Third Affiliated Hospital of Xuzhou Medical University from January 2024 to July 2025 were selected as research subjects,and were divided into comorbidity group(30 cases)and non-comorbidity group(57 cases)based on whether they experienced ALRTI.Binary Logistic regression analysis was used to investigate the influencing factors of COPD with ALRTI,and its prediction model was constructed.The receiver operator characteristic curve(ROC curve)was plotted and the area under ROC curve(AUC)was calculated.The predictive values of D-dimer,SAA,NT-proBNP alone and in combination for COPD with ALRTI were analyzed.Results D-dimer,SAA,homocysteine(HCY)and NT-proBNP were independent risk factors for COPD with ALRTI,with odds ratios(OR)of 3 026.595[95%confidence interval(95%CI)of 2.480-3 694 404.721],10.004(95%CI of 1.639-61.058),1.843(95%CI of 1.097-3.098)and 17.895(95%CI of 1.493-214.526)and P values of 0.027,0.013,0.021 and 0.023,respectively.The AUC of combined detection of D-dimer,SAA and NT-proBNP for prediction of COPD with ALRTI was 0.957(95%CI of 0.912-1.000),with sensitivity and specificity of 98.2%and 86.7%,respectively.The AUC of predictive model was 0.977(95%CI of 0.934-1.000),with sensitivity and specificity of 98.2%and 96.7%,respectively.Conclusion The individual and combined detection of D-dimer,SAA and NT-proBNP,as well as predictive model,had predictive values for COPD with ALRTI,and the predictive performance of the combined detection of three indicators was superior to that of each individual indicator.

Abstract:Objective To explore the efficacy of combination of Benazepril and Amlodipine Besylate in patients with primary hypertension.Methods The 60 patients with primary hypertension who were admitted to Guizhou Panjiang Coal and Electricity Group Co.,Ltd.Hospital from January 2023 to December 2024 were selected as study subjects and divided into control group(30 cases;treated with Amlodipine Besylate)and observation group(30 cases;treated with Benazepril in addition to control group regimen).After one month of treatment,the total effective rates were compared between two groups.Blood pressure[diastolic blood pressure(DBP)and systolic blood pressure(SBP)]was measured using sphygmomanometer in both groups before and after treatment,coagulation indicators[activated partial thromboplastin time(APTT)and fibrinogen(FIB)]were detected using automatic blood coagulation analyzer,and adverse reactions were observed and recorded.Differences in the levels of above indicators between two groups before and after treatment were compared.Results The total effective rate in observation group was significantly higher than that in control group(96.67%vs.73.33%,P<0.05).After treatment,the levels of DBP,SBP and FIB in observation group were significantly lower than those in control group,while APTT was significantly longer than that in control group,with statistically significant differences[DBP(mmHg;1 mmHg≈0.133 kPa):80.23±3.07 vs.85.15±3.23;SBP(mmHg):121.30±3.09 vs.136.27±6.15;FIB(g/L):2.65±0.22 vs.3.32±0.38;APTT(s):36.53±2.87 vs.34.10±2.50;all P<0.05].There was no significant difference in the incidence of adverse reactions between observation group and control group(16.67%vs.10.00%,P>0.05).Conclusions The combination of Benazepril and Amlodipine Besylate in patients with primary hypertension helps to enhance the antihypertensive effect,more stably regulate blood pressure levels,and promote the repair and improvement of coagulation function in patients,with good safety.

Abstract:Objective To investigate the diagnostic value of fibrinolytic markers and systemic immune inflammation index(SII)for venous thromboembolism(VTE)in patients with lung cancer.Methods The clinical characteristics and laboratory data were collected and analyzed from 103 hospitalised patients with confirmed lung cancer admitted to Longyan Second Hospital,from 1 October,2023 to 1 October,2024.Among them,15 cases were diagnosed with VTE within 6 months after lung cancer diagnosis and were included in VTE group.The remaining 88 cases of lung cancer did not develop VTE after 6 months and were included in non-thrombosis group.Plasma levels of thrombomodulin(TM),thrombin-antithrombin Ⅲ complex(TAT),plasmin-α2-plasmin inhibitor complex(PIC),tissue plasminogen activator-inhibitor 1 complex(t-PAIC)and SII between two groups were compared.Receiver operator characteristic curve(ROC curve)was plotted and area under ROC curve(AUC)was calculated to analyze the diagnostic efficacy of above markers for thrombotic events.Results The levels of TM,TAT,PIC and SII in VTE group were significantly higher than those in non-thrombotic group,with all differences being statistically significant[TM(TU/mL):13.70(10.85,14.95)vs.10.20(9.75,11.52);TAT(μg/L):12.96(5.85,24.02)vs.5.99(3.38,11.12);PIC(mg/L):1.01(0.79,2.03)vs.0.85(0.54,1.12);SII(×109/L):1 060.71(559.63,2 506.98)vs.241.02(368.38,704.23);all P<0.05].The ROC curve showed that AUC of TM,TAT,PIC and SII were 0.811,0.704,0.666 and 0.811,and the AUC of combined application of TM and SII increased to 0.819,with 95%confidence interval(95%CI)of 0.689-0.943.Conclusions Patients with lung cancer and VTE exhibit heightened fibrinolytic activity.Early monitoring of fibrinolytic markers TM,TAT,PIC and SII may prevent thrombosis and reduce thrombotic complications.

Abstract:Diabetes mellitus is a non infectious disease that disturbs human physical and mental health.If the disease condition is not clarified in time and no active and effective treatment and intervention measures are taken,it will not only cause irreversible damage to organs and tissues such as kidneys,hearts,eyes,etc.,but also lead to disability and even death of patients.Timely and accurate early diagnosis is the first step for clinical treatment of diabetes mellitus.With the continuous progress of medical technology,diagnostic methods based on detection of blood glucose,urine glucose,glycosylated hemoglobin(HbA1c)and other indicators play an important role in the diagnosis and treatment of diabetes mellitus.This article mainly analyzed the application status of HbA1c and fasting blood glucose(FBG)in the examination of diabetes mellitus,and provided a reference basis for future related research and clinical application.

Abstract:Objective To analyze the level of 25-hydroxyvitamin D[25(OH)D]in children in Chifeng area,Inner Mongolia Autonomous Region,explore its correlation with serum calcium(Ca),phosphorus(P)and alkaline phosphatase(ALP),and provide a reference for the nutritional and health status of children in this area.Methods The biochemical index test results of 806 children aged 0-13 who underwent physical examination at Chifeng Songshan Hospital from June to December 2024 were collected,including serum 25(OH)D,Ca,P and ALP.According to age,the children were divided into 1-3 years old group(145 cases),4-6 years old group(219 cases)and 7-13 years old group(442 cases).They were also divided into 25(OH)D deficiency group(136 cases),25(OH)D insufficiency group(200 cases)and 25(OH)D sufficiency group(470 cases)based on 25(OH)D levels.The baseline data from each group and factors affecting 25(OH)D in children were analyzed,and the correlations between 25(OH)D levels and serum Ca,P and ALP levels were investigated using Pearson correlation analysis.Results Among boys of 1-3 years old group,4-6 years old group and 7-13 years old group,the proportions of 25(OH)D deficiency were 3.5%,10.5%and 23.1%,respectively,while the proportions of 25(OH)D sufficiency were 74.7%,61.7%and 52.7%,respectively.Among girls of 1-3 years old group,4-6 years old group and 7-13 years old group,the proportions of 25(OH)D deficiency were 3.4%,15.1%and 24.2%,respectively,the proportions of 25(OH)D insufficiency were 12.1%,22.1%and 30.3%,respectively,the proportions of 25(OH)D sufficiency were 84.5%,62.8%and 45.5%,respectively,and the differences were statistically significant(all P<0.05).Compared with children in 1-3 years old group,the 25(OH)D levels in children of 4-6 years old group and 7-13 years old group decreased significantly(μg/L:34.94±12.83 vs.24.93±7.51,21.94±6.78,both P<0.001),and the level of 25(OH)D in 7-13 years old group was significantly lower than that in 4-6 years old group(P<0.05).Compared with 1-3 years old group,the serum levels of Ca in 4-6 years old group and 7-13 years old group were significantly reduced(mmol/L:2.53±0.12 vs.2.45±1.04,2.45±0.12,both P<0.001).The serum levels of Ca in 25(OH)D sufficiency group were significantly higher than those in 25(OH)D deficiency group and 25(OH)D insufficiency group(mmol/L:2.53±0.21 vs.2.25±0.16,2.10±0.22,both P<0.05),while the serum levels of ALP were significantly lower than those in 25(OH)D deficiency group and 25(OH)D insufficiency group(mmol/L:207.00±63.89 vs.225.30±91.23,218.00±65.62,both P<0.05).The Pearson correlation analysis results showed that serum 25(OH)D was positively correlated with Ca,and negatively correlated with serum ALP(r values were 0.61 and-0.49,P values were 0.031 and 0.028,respectively).Conclusion Children in different age groups in Chifeng area have varying degrees of vitamin D deficiency,which is related to the season and serum Ca and ALP.

Abstract:Pulse wave velocity and pulse contour analysis technology is a joint application of non-invasive hemo-dynamic monitoring technology,which can accurately capture subtle changes in blood vessel hardness and elasticity,and conduct a comprehensive and accurate assessment of core elements of cardiac function from a hemodynamic perspective.At present,there is a lack of expert consensus on the application of pulse wave velocity and pulse contour analysis technology in clinical practice.In order to standardize the clinical application of pulse wave velocity and pulse contour analysis technology,25 experts from clinical medicine,evidence-based medicine and other fields were invited to form an expert group to discuss and determine the current clinical issues that urgently need to be standardized and unified in the application process of this technology.By referring to relevant domestic and foreign literature,combined with expert clinical experience,and revised after discussion by an expert group,the"Expert consensus on clinical application of non-invasive hemodynamics-pulse wave velocity and pulse contour analysis technology"was finally formed,which provides evidence-based basis for clinical non-invasive hemodynamic management and will be continuously updated as evidence accumulates.

Abstract:Objective To explore the clinical diagnostic value of combined detection of inflammatory markers in pediatric bacterial pneumonia and mycoplasma pneumonia.Methods The clinical data of 65 cases of pediatric bacterial pneumonia and 85 cases of mycoplasma pneumonia admitted to Jiading District Central Hospital Affiliated to Shanghai University of Medicine and Health Sciences from January 1 to August 31,2025 were collected.Additionally,16 cases with normal physical examination results were included as control group.The levels of serum procalcitonin(PCT),serum amyloid A(SAA)and blood C-reactive protein(CRP)were detected in all subjects,and the levels of above indicators among three groups were compared.Receiver operator characteristic curve(ROC curve)was plotted and area under ROC curve(AUC)was calculated to analyze the clinical values of inflammatory markers in assisting the diagnosis of pediatric bacterial pneumonia and mycoplasma pneumonia.Results The levels of SAA,CRP and PCT in bacterial pneumonia group were significantly higher than those in mycoplasma pneumonia group[SAA(mg/L):136.00(82.67,288.18)vs.19.70(10.4,49.65);CRP(mg/L):17.65(11.65,28.71)vs.3.05(1.11,7.17);PCT(μg/L):0.15(0.08,0.31)vs.0.08(0.05,0.15);all P<0.05].For distinguishing pediatric bacterial pneumonia from mycoplasma pneumonia,the AUC values of SAA,CRP and PCT were 0.912[95%confidence interval(95%CI)was 0.869-0.956],0.856(95%CI was 0.791-0.921)and 0.688(95%CI was 0.603-0.774),respectively.The AUC of combined detection of three indicators was 0.928(95%CI was 0.886-0.969).Conclusion PCT,SAA and CRP have high efficacies in distinguishing pediatric bacterial pneumonia from mycoplasma pneumonia,and they are of great significance for disease diagnosis.

Abstract:Objective To explore the changes of blood cell parameters and serum iron(SI)level in pregnant women with β-thalassemia and the application value in differential diagnosis of the disease.Methods The 58 pregnant women with anemia who underwent regular prenatal examinations at Longyan People's Hospital from January to November 2024 were selected as research subjects.According to the type of anemia,the patients were divided into thalassemia group(24 cases)and iron deficiency anemia group(34 cases).The thalassemia group was further divided into β-thalassemia group(14 cases)and α-thalassemia group(10 cases).Additionally,82 healthy pregnant women who underwent prenatal examinations during the same period were selected as control group.All subjects were tested for peripheral blood cell parameters[mean corpuscular hemoglobin(MCH)and mean corpuscular volume(MCV)],SI and hemoglobin A2(HbA2)using blood cell analyzer and electrophoresis.The differences in levels of above indicators among pregnant women with different types of anemia were compared.Receiver operator characteristic curve(ROC curve)was drawn and area under ROC curve(AUC)was calculated to analyze the diagnostic value of MCH,MCV,SI and HbA2 alone and in combination for β-thalassemia in pregnant women.Results The levels of MCH and MCV in thalassemia group were lower than those in control group and iron deficiency anemia group[MCH(pg):21.52±3.13 vs.30.03±3.56,26.20±4.55;MCV(fL):68.11±6.28 vs.90.23±5.02,76.85±3.44;all P<0.05],and the levels of MCH and MCV in iron deficiency anemia group were lower than those in control group(all P<0.05).The levels of SI in thalassemia group and control group were higher than that in iron deficiency anemia group(μmol/L:19.12±4.33,18.35±5.57 vs.10.75±2.02,both P<0.05).The level of HbA2 in thalassemia group was significantly higher than those in control group and iron deficiency anemia group(0.031±0.010 vs.0.028±0.002,0.028±0.003,both P<0.05).The levels of MCH and MCV in β-thalassemia group were lower than those in α-thalassemia group,and the level of HbA2 was higher than that in α-thalassemia group[MCH(pg):20.15±2.42 vs.22.43±2.30;MCV(fL):67.06±2.94 vs.69.79±3.23;HbA2:0.039±0.006 vs.0.022±0.003;all P<0.05].The predictive efficacy of MCH,MCV,SI and HbA2 alone for β-thalassemia in pregnant women was relatively low,while the combined detection of MCH,MCV and SI had a higher predictive efficacy,with AUC of 0.707,sensitivity of 85.71%,and specificity of 56.82%.The combined detection of four indicators had the highest predictive efficacy for β-thalassemia in pregnant women,with AUC of 0.724,sensitivity of 92.86%,and specificity of 47.73%.Conclusions There are certain differences in MCH,MCV,SI and HbA2 levels among different types of pregnant women with thalassemia.The combined detection of MCH,MCV,SI and HbA2 is helpful for the diagnosis of β-thalassemia during pregnancy.The combined detection of MCH,MCV,SI and HbA2 has the highest diagnostic efficiency for β-thalassemia during pregnancy.

Abstract:Objective To analyze the nutritional health status of 642 children in Hohhot,Inner Mongolia Autonomous Region,and its correlation with serum urea nitrogen(SUN)and alkaline phosphatase(ALP).Methods A total of 642 children who visited the pediatrics department of Inner Mongolia Autonomous Region Maternal and Child Health Hospital from March 2024 to June 2025 were selected as research objects.They were divided into malnutrition group(28 cases;including 16 cases aged 0-3 years,6 cases aged 4-7 years and 6 cases aged 8-12 years)and healthy control group(614 cases;including 312 cases aged 0-3 years,192 cases aged 4-7 years and 110 cases aged 8-12 years).The clinical data of research subjects were collected and analyzed,the gender and age distribution of children in malnourished group and healthy control group were analyzed,and the differences in body weight,SUN and ALP levels between two groups were compared.Results There was no statistically significant difference in gender distribution between malnourished group and healthy control group.In malnourished group,the proportions of children aged 0-3 years,4-7 years and 8-12 years were 57.14%,21.43%and 21.43%,and in healthy control group,the proportions of children aged 0-3 years,4-7 years and 8-12 years were 50.81%,31.27%and 17.92%,respectively,with statistically significant differences(P<0.05).The body weight,SUN and ALP levels in malnutrition group were significantly lower than those in healthy control group[body weight(kg):7.21±2.57 vs.13.26±0.85;SUN(μmol/L):3.16±1.34 vs.5.80±5.08;ALP(U/L):182.83±67.05 vs.290.75±162.16;all P<0.05].Conclusions In Hohhot area,the proportion of children aged 0-3 years in malnourished group is 57.14%,which is significantly higher than those of other age groups.The main manifestations are low body weight and reduced SUN and ALP levels,which may be the risk factors for malnutrition in this region.

Abstract:Objective To investigate the preventive effect of Maitongning Decoction combined with low molecular weight heparin calcium on lower extremity deep vein thrombosis(DVT)in patients with thoracolumbar fracture after surgery and its impact on coagulation function.Methods A total of 60 patients with thoracolumbar fractures after surgery at the Second Affiliated Hospital of Guizhou University of Chinese Medicine from June 2024 to June 2025 were selected as research objects,and divided into control group(30 cases;treated with low molecular weight heparin calcium after surgery)and observation group(30 cases;received Maitongning Decoction in addition to control group).The incidence of lower extremity DVT,levels of coagulation function parameters[prothrombin time(PT),activated partial thromboplastin time(APTT)and D-dimer],lower extremity circumference difference and incidence of adverse reactions were compared between two groups.Results The incidence of lower extremity DVT in observation group was significantly lower than that in control group at 14 days postoperatively[6.67%(2/30)vs.26.67%(8/30),P<0.05].At 14 days postoperatively,the PT and APTT in observation group were significantly longer than those in control group,while the level of D-dimer and lower extremity circumference difference were lower than those in observation group[PT(s):14.82±1.26 vs.13.65±1.14;APTT(s):36.84±4.15 vs.33.27±3.86;D-dimer(mg/L):0.98±0.32 vs.1.57±0.45;thigh circumference difference(cm):1.05±0.43 vs.1.68±0.52;all P<0.05].There was no statistically significant difference in the incidence of adverse reactions between two groups[20.0%(6/30)vs.13.3%(4/30),P>0.05].Conclusions Maitongning Decoction combined with low molecular weight heparin calcium can effectively reduce the incidence of lower extremity DVT in patients with thoracolumbar fracture after surgery,improve coagulation function,and alleviate lower limb swelling,with good safety.It is worthy of promotion in clinical practice.

Abstract:Objective To explore the blood tandem mass spectrometry indexes of newborns with primary carnitine deficiency(PCD)in Foshan City,Guangdong Province,improve the sensitivity of early screening,analyze the characteristics of SLC22A5 gene mutation spectrum and serum biochemical indicators,and provide scientific basis for the early diagnosis and precise treatment of PCD in the region.Methods The samples of heel blood were collected from neonates in 38 midwifery institutions in Foshan City from January 2020 to December 2024 to screen multiple inherited metabolic diseases using tandem mass spectrometry.The suspected neonates with free carnitine(C0)<laboratory cut-off value and their mothers were recalled simultaneously,and gene detection was performed on those with re-screening results positive.For neonates confirmed with PCD,positive rates of clinical biochemical indexes[lactate dehydrogenase(LDH)and total bile acid(TBA)]and gene mutation characteristics were analyzed.Results Among the 305 297 neonates undergoing tandem mass spectrometry screening,there were 638 cases with blood C0<normal reference value(cut-off value C0<12 μmol/L)and 542 cases were recalled,with a recall rate of 84.9%.There were 12 neonates diagnozed with PCD,with an incidence of 0.004%.The data analysis showed that C0 level was relatively low,which was(3.66-10.15)μmol/L in the initial screening,and C0 was positively correlated with 16-acylcarnitine(C16;r=0.750,P=0.005).In neonates with PCD,there were 3 mothers with C0 decrease(<14.00 μmol/L),3 mothers with normal C0 and 2 mothers with C16 or 18-acylcarnitine(C18)abnormality.After levocarnitine treatment,C0 level was significantly higher than that before treatment(μmol/L:31.33±11.77 vs.7.28±2.11,P<0.001).Among the 542 neonates with initial screening and re-screening results positive,there were 10 types of SLC22A5 gene mutation,and incidence of c.51C>G(p.F17L)and c.1400C>G(p.S467C)mutation was higher[both accounting for 50.0%(6 cases)].All families were conformed to autosomal recessive inheritance pattern.There were 5 mutation carriers,and frequency of c.51C>G(p.F17L)mutation was 3 times,accounting for 60.0%.The biochemical examination results showed that the positive detection rates of LDH and TBA were both 58.33%(7/12).Conclusions There are specific lineage characteristics of gene mutation in neonates with PCD in Foshan City,c.51C>G and c.1400C>G are main mutation hotspots.Tandem mass spectrometry combined with genetic analysis can effectively increase early diagnostic rate,LDH and TBA can be applied as auxiliary diagnostic indexes.Levocarnitine treatment is effective for PCD,but long-term standardized treatment and follow-up are also necessary.

Abstract:Objective To analyze changes in complement C3,C4 and immunoglobulin(Ig)levels in patients with rheumatoid arthritis(RA).Methods The clinical data of 235 patients with RA in Longyan People's Hospital from January 2024 to January 2025 were retrospectively collected and included in observation group.Based on RA disease activity score(DAS28),the patients were categorized into high-activity group(55 cases;DAS28>5.1),moderate-activity group(59 cases;3.2<DAS28≤5.1),low-activity group(61 cases;2.6<DAS28≤3.2)and remission group(60 cases;DAS28≤2.6).Additionally,60 healthy individuals undergoing physical examinations during the same period were selected as control group.The levels of complement C3,C4 and IgA,IgM,IgG were detected using immunoturbidimetry,and the differences in above indicators between observation group and control group,and among patients with different disease activities in observation group were compared.Pearson correlation analysis was employed to evaluate the relationship between disease activity and above indicators.Results The observation group showed significantly higher levels of complement C3,C4 and IgA,IgM,IgG compared to control group[complement C3(g/L):1.81±0.46 vs.0.92±0.23;complement C4(g/L):0.62±0.08 vs.0.41±0.11;IgA(g/L):4.23±1.08 vs.1.49±0.36;IgM(g/L):3.84±1.06 vs.1.34±0.35;IgG(g/L):16.44±3.76 vs.11.94±1.78;all P<0.05].The levels of complement C3,C4 and IgA,IgM,IgG in high-activity group were significantly higher than those in moderate-activity group,low-activity group and remission group[complement C3(g/L):2.34±0.52 vs.2.07±0.46,1.80±0.48,1.17±0.33;complement C4(g/L):0.76±0.08 vs.0.70±0.09,0.60±0.07,0.43±0.05;IgA(g/L):6.25±1.29 vs.4.97±1.12,3.63±0.95,2.26±0.60;IgM(g/L):4.79±1.64 vs.4.25±0.98,3.59±0.74,2.83±0.58;IgG(g/L):22.84±4.39 vs.17.59±3.66,14.14±3.31,11.79±3.24;all P<0.05].The moderate-activity group showed significantly higher levels of complement C3,C4 and IgA,IgM,IgG than low-activity group and remission group,while the low-activity group exhibited significantly higher levels of above markers compared to remission group(all P<0.05).Pearson correlation analysis revealed positive correlations between disease activity and complement C3,C4 and IgA,IgM,IgG levels(r values were 0.404,0.462,0.397,0.415 and 0.554,P values were 0.007,<0.001,0.008,0.010 and<0.001,respectively).Conclusions The disease activity of patients with RA is closely associated with the complement and antibody markers.Monitoring these indicators could provide valuable insights for clinical assessment and treatment.

Abstract:Objective To investigate the serum levels of 25-hydroxyvitamin D[25(OH)D]in healthy children aged 0-3 years in Changsha area of Hunan Province and analyze their vitamin D(VD)nutritional status according to different genders,ages and testing seasons.Methods A total of 8 695 healthy children aged 0-3 years who underwent routine physical examinations at Hunan Children's Hospital from January 2022 to December 2024 were selected as research objects,including 5 100 males and 3 595 females.According to age,they were divided into 0-<1 year old group(1 922 cases),1-<2 years old group(3 028 cases),and 2-3 years old group(3 745 cases).According to testing seasons,they were divided into spring group(2 345 cases;March,April and May),summer group(3 057 cases;June,July and August),autumn group(1 878 cases;September,October and November)and winter group(1 415 cases;December,January and February).Serum 25(OH)D was measured using chemiluminescence,and the levels of 25(OH)D and prevalence of insufficiency and deficiency of VD were compared across groups.Results The overall serum 25(OH)D level was 130.22(105.42,156.69)nmol/L.The prevalence of VD deficiency,insufficiency and sufficiency was 0.44%,3.98%and 95.58%,respectively.The serum 25(OH)D level in girls was higher than that in boys[nmol/L:131.99(105.89,158.63)vs.129.00(105.14,155.50),P<0.05].There was statistically significant difference in serum 25(OH)D levels among different age groups,with the 25(OH)D level in 1-<2 years old group significantly higher than those in 0-<1 year old group and 2-3 years old group[nmol/L:145.68(123.40,171.08)vs.128.27(106.10,152.07),117.40(94.77,143.30),both P<0.05].The serum 25(OH)D level in children was higher in autumn than that in spring,and the difference was statistically significant[nmol/L:139.20(115.65,164.33)vs.125.96(99.33,152.84),P<0.05].The proportion of VD insufficiency and deficiency in children was higher in spring than that in autumn,and the difference was statistically significant[6.18%(145/2 345)vs.1.86%(35/1 878),P<0.05].Conclusions VD status is generally adequate among healthy children aged 0-3 years in Changsha area.Girls have higher 25(OH)D levels than boys.Children aged 1-<2 years have the highest 25(OH)D levels,while those aged 2-3 years are at increased risk of VD insufficiency and deficiency.The level of 25(OH)D in children is the highest in autumn and the lowest in spring.

Abstract:Objective To analyze the risk factors of diabetes mellitus combined with atherosclerosis(AS),and to construct and validate the Nomogram prediction model.Methods A total of 894 diabetic patients admitted to the First People's Hospital of Dali from January 2021 to August 2023 were selected as research subjects.According to whether there was AS,they were divided into non combined group(417 cases)and combined AS group(477 cases).The demographic data and clinical characteristics of two groups were compared.Multivariate Logistic regression analysis was used to explore the independent influencing factors of diabetes combined with AS.Nomogram prediction model was constructed,receiver operator characteristic curve(ROC curve)and correction curve were plotted,and area under ROC curve(AUC)was calculated to evaluate the predictive efficacy of the model.Results Age,glycated serum protein(GSP),high-density lipoprotein cholesterol(HDL-C),hypertension and hyperlipidemia were independent risk factors for diabetes mellitus combined with AS[odds ratios(OR)were 2.67(Q4),2.54(Q4),0.55(Q4),1.95 and 2.84,and P values were<0.001,<0.001,0.018,0.002 and<0.001,respectively].The Nomogram prediction model had an AUC of 0.712[95%incidence interval(95%CI)was 0.672-0.752]of training set,with specificity and sensitivity of 59.2%and 72.4%,respectively.The AUC in validation set was 0.695(95%CI was 0.632-0.757),and the specificity and sensitivity were 73.7%and 59.6%,respectively.The calibration curve showed that the average absolute errors of model prediction in training set and validation set were 0.017 and 0.012,respectively.Conclusions Age,GSP,HDL-C,hypertension and hyperlipidemia are independent influencing factors for diabetes mellitus combined with AS,and the Nomogram prediction model based on these risk factors has certain predictive value.

Abstract:Objective To explore the changes of postoperative serum cytokeratin 19 fragment antigen(CYF21-1),neuron-specific enolase(NSE)and carbohydrate antigen 125(CA125)levels in patients with lung cancer and analyze their relationship with recurrence within 1 year.Methods A total of 137 patients with lung cancer who received radical resection admitted in Shandong Yiyang Healthcare Group Xinwen Central Hospital from April 2021 to February 2024 were included as study subjects.The changes of serum CYF21-1,NSE and CA125 levels were compared before surgery and at 1,2 and 3 weeks after surgery.According to the cancer recurrence status within 1 year of follow-up,the patients were divided into recurrence group(31 cases)and non-recurrence group(106 cases).The levels of serum CYF21-1,NSE and CA125 at 3 weeks after surgery were compared between two groups.The influencing factors of recurrence within 1 year after surgery in patients with lung cancer were analyzed by binary Logistics regression.Receiver operator characteristic curve(ROC curve)was drawn and area under ROC curve(AUC)was calculated to analyze the predictive values of serum CYF21-1,NSE and CA125 at 3 weeks after surgery on recurrence within 1 year after surgery in patients with lung cancer.Results The proportion of patients with TNM stage ⅢA in recurrent group was higher than that in non-recurrent group(61.29%vs.34.91%,P<0.05).Within 3 weeks after surgery,the serum levels of CYF21-1,NSE and CA125 were decreased significantly and continuously.The levels of serum CYF21-1,NSE and CA125 before surgery and at 3 weeks after surgery were significantly higher than those in non-recurrence group[before surgery:CYF21-1(μg/L):19.47±4.06 vs.17.12±3.76,NSE(μg/L):29.67±4.56 vs.27.69±4.15,CA125(kU/L):228.54±47.12 vs.207.23±40.57;at 3 weeks after surgery:CYF21-1(μg/L):4.87±0.95 vs.4.25±0.78,NSE(μg/L):16.93±3.24 vs.14.94±2.95,CA125(kU/L):65.07±14.65 vs.53.74±12.04;all P<0.05].Logistic regression analysis showed that proportion of TNM stage ⅢA and CYF21-1,NSE and CA125 before surgery and at 3 weeks after surgery were associated with recurrence of lung cancer patients within 1 year after surgery[odds ratios(OR)were 5.697,2.662,10.892,1.112,1.879,8.837,1.706,P values were 0.039,0.026,0.024,0.042,0.008,0.008,<0.001].The AUCs of CYF21-1,NSE and CA125 at 3 weeks after surgery in predicting recurrence within 1 year after surgery were 0.676,0.631 and 0.653,respectively.The AUC,sensitivity and specificity of combined diagnosis were 0.766,80.65%and 55.66%,respectively,and the combined diagnostic value was higher.Conclusion The levels of serum CYF21-1,NSE and CA125 in patients with lung cancer after surgery will decrease significantly,and serum CYF21-1,NSE and CA125 at 3 weeks after surgery have certain predictive value on recurrence of lung cancer within 1 year.

Abstract:Objective To investigate the clinical value of continuous positive airway pressure(CPAP)in treatment of non-arteritic anterior ischemic optic neuropathy(NAION)combined with moderate to severe obstructive sleep apnea hypopnea syndrome(OSAHS),and clarify its related therapeutic mechanism.Methods From June 2024 to May 2025,the 40 patients diagnosed with NAION combined with moderate to severe OSAHS in Shanxi Fenyang Hospital were selected for study,and divided into treatment group(20 cases;receiving conventional medication combined with CPAP)and control group(20 cases;receiving conventional medication).During the 30-day treatment period,the changes in core parameters such as nocturnal apnea hypopnea index(AHI),minimum oxygen saturation(MOS)and visual acuity and mean deviation(MD)of visual field in both groups were monitored and recorded.Results The total effective rate of treatment group was significantly higher than that of control group[90.0%(18/20)vs.30.0%(6/20),P<0.05].After treatment,the levels of AHI,MOS,MD and visual acuity in treatment group were significantly better than those before treatment[AHI(times/hour):3.5±1.8 vs.32.3±5.2;MOS:(92.5±3.2)%vs.(75.2±4.5)%;MD(dB):-10.3±3.2 vs.-17.5±5.8;visual acuity(LogMAR):0.32±0.13 vs.0.58±0.18;all P<0.05].After treatment,the levels of AHI,MOS,MD and visual acuity in treatment group were significantly better than those in control group[AHI(times/hour):3.5±1.8 vs.31.9±4.7;MOS:(92.5±3.2)%vs.(78.2±2.3)%;MD(dB):-10.3±3.2 vs.-16.3±3.5;visual acuity(LogMAR):0.32±0.13 vs.0.53±0.11;all P<0.05].Conclusions Adding CPAP treatment to conventional medication could more effectively manage NAION combined with moderate to severe OSAHS.Moreover,the nocturnal NAION symptoms in OSAHS patients are closely related to hypoxia,revealing that hypoxia may be the main cause of such lesions.

Abstract:Objective To explore the effect of meglumine cyelic adenosine injection combined with"Golden Triangle"regimen in the treatment of chronic heart failure(CHF).Methods A total of 88 patients with CHF admitted to Jiangle County General Hospital from January 2022 to February 2024 were selected as research subjects and randomly divided into combined treatment group(44 cases;receiving meglumine cyelic adenosine injection combined with"Golden Triangle"regimen)and conventional treatment group(44 cases;receiving"Golden Triangle"regimen).Cardiac function indexes[left ventricular ejection fraction(LVEF),left ventricular end-systolic diameter(LVESD)and left ventricular end-diastolic diameter(LVEDD)]were measured using ultrasound system.Inflammatory factors[interleukin-1β(IL-1β)and interleukin-18(IL-18)]and myocardial injury markers[N-terminal pro-brain natriuretic peptide(NT-proBNP)and cardiac troponinⅠ(cTnⅠ)]were measured using enzyme linked immunosorbent assay.The efficacy was assessed based on New York Heart Association(NYHA)cardiac function grading criteria,and adverse reactions such as gastrointestinal discomfort and hypotension were recorded.Results After treatment,the level of LVEF in combination treatment group was higher than that in conventional treatment group,while the levels of LVESD and LVEDD were lower than those in conventional treatment group[LVEF:(44.71±4.51)%vs.(40.91±4.02)%;LVESD(mm):35.01±3.61 vs.39.79±4.02;LVEDD(mm):45.02±4.61 vs.48.69±4.91;all P<0.05].After treatment,the levels of IL-1β,IL-18,NT-proBNP and cTnⅠ in combination treatment group were lower than those in conventional treatment group[IL-1β(ng/L):5.52±0.56 vs.6.43±0.63;IL-18(ng/L):214.64±22.02 vs.256.74±26.33;NT-proBNP(ng/L):381.64±37.26 vs.416.97±42.61;cTnⅠ(μg/L):1.52±0.16 vs.1.74±0.18;all P<0.05].The total effective rate of combination treatment group was higher than that of conventional treatment group(90.91%vs.75.00%,P<0.05),while the total incidence of adverse reactions showed no statistically significant difference between the two groups(15.91%vs.11.36%,P>0.05).Conclusions Meglumine cyelic adenosine injection combined with"Golden Triangle"regimen can significantly improve the cardiac function of patients with CHF,inhibit their inflammatory response and reduce myocardial damage,and the combined use of drugs does not significantly increase the adverse reactions of patients.

Abstract:Objective To analyze the therapeutic effect of fiberoptic bronchoscopy bronchoalveolar lavage combined with rivastigmine on burn patients with inhalation injury.Methods A total of 68 patients with burn combined with inhalation injury who received treatment in Suzhou Municipal Hospital were selected as research subjects.The time period of cases was from January 2022 to December 2024.Based on different treatment methods,the patients were divided into control group(33 cases;treated with conventional method)and study group(35 cases;treated with fiberoptic bronchoscopy bronchoalveolar lavage combined with rivastigmine).The changes in inflammatory indicators[interleukin(IL),tumor necrosis factor-α(TNF-α)and C-reactive protein(CRP)]and coagulation parameters[D-dimer and fibrinogen(FIB)]before and after treatment were compared and analyzed between two groups.Results Compared with control group,the study group showed significantly shorter hospital stay and intensive care unit(ICU)stay[hospital stay(days):36.45±3.12 vs.40.45±3.90;ICU stay(days):7.02±2.01 vs.9.67±2.30;both P<0.05].After 1 week of treatment,the respiratory rate and heart rate in study group were significantly lower than those in control group,while oxygen saturation was significantly higher[respiratory rate(times/minute):22.60±1.01 vs.27.34±1.59;heart rate(beats/minute):98.40±2.56 vs.110.78±3.45;oxygen saturation:(97.68±5.71)%vs.(89.67±4.04)%;all P<0.05].After 1 week of treatment,the levels of related inflammatory and coagulation markers in study group were significantly lower than those in control group[IL-6(ng/L):26.64±1.60 vs.34.04±2.31;CRP(mg/L):5.45±2.31 vs.10.90±3.32;D-dimer(mg/L):1.28±0.54 vs.2.50±0.90;FIB(g/L):3.53±0.85 vs.4.08±0.94;all P<0.05].After treatment,the levels of TNF-α,IL-6 and IL-8 in bronchoalveolar lavage fluid(BALF)of study group were significantly lower than those in control group,while the level of IL-10 was significantly higher[TNF-α(ng/L):32.18±7.26 vs.45.21±9.43;IL-6(ng/L):65.27±12.98 vs.82.45±15.32;IL-8(ng/L):40.35±8.75 vs.54.37±10.21;IL-10(ng/L):33.72±6.84 vs.24.56±5.21;all P<0.05].The total effective rate in study group was significantly higher than that in control group[94.29%(33/35)vs.75.76%(25/33),P<0.05].Conclusions Treatment with fiberoptic bronchoscopy bronchoalveolar lavage combined with rivastigmine in patients with burn and inhalation injury could stabilize the condition more rapidly and improve the body's inflammatory state and coagulation disorder.

Abstract:With the continuous advancement of medical technology,the level of clinical laboratory testing has significantly improved,and its frequency in medical practice has increased.Blood testing is frequently used in clinical settings,being the most widely applied method.Compared to other testing methods,blood testing includes a broader range of indicators.The blood analyzer is a crucial instrument for conducting blood tests,known for its comprehensive functions and high accuracy.However,practical feedback indicates that this instrument can be easily affected by external interference during use,leading to inaccurate test results.The application of blood smear method compensates for the limitations of blood analyzers and has made significant contributions to diagnosing blood-borne diseases.In order to further improve the application value of blood smear test in disease diagnosis,this article reviews and analyzes the application of blood smear test in diagnoses for different diseases and its combined application value in blood analyzers.