Abstract:Objective To explore the therapeutic effect of Xiaoyin granules combined with halometasone cream in the treatment of psoriasis and its impact on immune function.Methods A total of 128 psoriasis patients admitted to the Air Force Medical Center of PLA from March 2022 to March 2024 were selected and divided by random number table method into:a control group(64 cases)treated with Xiaoyin granules,and a study group(64 cases)treated with Xiaoyin granules plus halometasone cream.The efficacy,Psoriasis Area and Severity Index(PASI)score,CD4+,CD8+,immunoglobulin(Ig)A,IgG levels,and incidence of adverse reactions were compared between the two groups before and after treatment.Results The total effective rate in the control group was significantly lower than that in the study group(P<0.05).After treatment,the total PASI score and regional PASI scores of both groups decreased significantly,and the scores in the study group were lower than those in the control group(P<0.05).After treatment,CD4+level and CD4+/CD8+ratio increased significantly in both groups(P<0.05),while CD8+,IgA,and IgG levels decreased significantly(P<0.05).The study group showed significantly higher CD4+level and CD4+/CD8+ratio than the control group(P<0.05),and significantly lower CD8+,IgA,and IgG levels(P<0.05).The total incidence of adverse reactions was 9.38%in both the control group and the study group,with no statistically significant difference(P>0.05).Conclusion The combination of Xiaoyin granules and halometasone cream is effective in the treatment of psoriasis,can alleviate skin lesions,regulate immune function,and has a high safety profile.

Abstract:Mitochondria,serving as cellular powerhouses and metabolic control centers,play a crucial role in maintaining cellular homeostasis.Mitochondrial quality control(MQC)is a core defense mechanism for preserving mitochondrial health.It regulates the relative stability of mitochondrial morphology,number,and quality through mitophagy,fission,and fusion,thereby sustaining cellular homeostasis.Mitochondrial-derived vesicles(MDVs)represent a newly identified MQC mechanism involved in regulating mitochondrial oxidative stress,widely present across diverse cell types and tissues.Under oxidative stress conditions,MDVs are extensively induced.They selectively encapsulate damaged mitochondrial components and transport them to lysosomes for degradation,thereby mediating the early clearance of damaged materials and effectively preserving mitochondrial structural and functional integrity.This review systematically summarizes the biogenesis,cargo selection,and transport pathways of MDVs,with a focus on elucidating the physiological roles of MDVs and recent research advances in their involvement in various diseases.

Abstract:Chronic respiratory diseases represented by chronic obstructive pulmonary disease,pulmonary fibrosis,and lung cancer have become a major challenge in global public health due to their high incidence,high disability and mortality rates,and heavy socioeconomic burden.There remain shortcomings in the understanding of pathogenesis and the development of new diagnostic and therapeutic strategies for such diseases,and traditional treatment models cannot fully meet clinical needs.Against this backdrop,hydrogels,as biomaterials with tunable mechanical properties and excellent biocompatibility,have made progress in fields such as drug delivery and tissue engineering,demonstrating broad application prospects.This article reviews the applications of hydrogels in common respiratory diseases and discusses their potential and advantages in elucidating disease mechanisms,treatment,and prevention.

Abstract:Objective To compare the effects of low-frequency transcutaneous electrical nerve stimulation and balance acupuncture at the lumbago acupoint on pain relief and lumbar function improvement in patients with training-induced low back pain at the primary care level.Methods A total of 72 patients with training-induced low back pain admitted to First Medical Center of PLA General Hospital from October 2024 to April 2025 were prospectively selected and divided into a low-frequency transcutaneous electrical nerve stimulation(TENS)group(36 cases)and a balance acupuncture at the lumbago acupoint group(36 cases)using a random number table.Both groups received their respective treatments for a course of 2 weeks.General data were compared between the two groups.Visual Analogue Scale(VAS)and Oswestry Disability Index(ODI)scores were compared before and after treatment.Results Repeated-measures analysis of variance showed a significant time×group interaction effect on VAS scores(F=4.231,P=0.021).There was no statistically significant difference in VAS scores between the two groups before treatment and at the 7-day follow-up(P>0.05).After treatment and at the 3-day and 7-day follow-ups,VAS scores in both groups were significantly lower than before treatment(P<0.05).After treatment and at the 3-day follow-up,the VAS scores of the balance acupuncture at the lumbago acupoint group[(1.19±1.69)points,(1.14±1.29)points]were significantly lower than those of the low-frequency TENS group[(2.06±1.58)points,(1.81±1.41)points](P<0.05).Repeated-measures analysis of variance showed a significant time×group interaction effect on ODI scores(F=3.894,P=0.035).There was no statistically significant difference in ODI scores between the two groups before treatment(P>0.05).After treatment and at the 3-day and 7-day follow-ups,ODI scores in both groups were significantly lower than before treatment,and the scores of the balance acupuncture at the lumbago acupoint group were significantly lower than those of the low-frequency TENS group(P<0.05).Conclusion Both low-frequency transcutaneous electrical nerve stimulation and balance acupuncture at the lumbago acupoint can effectively alleviate training-induced low back pain and improve lumbar function.Balance acupuncture at the lumbago acupoint is superior to low-frequency transcutaneous electrical nerve stimulation in terms of onset speed and therapeutic intensity.

Abstract:Objective To evaluate the scanning time,positioning accuracy,and consistency of cardiac function results between the EasyScan automatic positioning technology and the traditional manual positioning method,aiming to verify the potential of this technology in improving examination efficiency and maintaining image quality.Methods Sixty-five consecutive patients who underwent cardiac magnetic resonance(CMR)examination at the Tongzhou Campus of Beijing Friendship Hospital Affiliated to Capital Medical University from October 2024 to March 2025 were prospectively enrolled.Among them,6 patients did not undergo contrast-enhanced examination,and the remaining 59 patients completed the scanning of all sequences.Each patient underwent traditional manual positioning and EasyScan automatic positioning scans both before and after contrast enhancement.The efficacy of the three positioning scenarios was comprehensively evaluated by comparing the following indicators:the angle between the positioning lines of each plane and the anatomical reference lines(objectively measured),subjective image scores,cardiac function parameters[including left ventricular end-diastolic volume(LVEDV),left ventricular end-systolic volume(LVESV),left ventricular ejection fraction(LVEF),left ventricular stroke volume(LVSV),left ventricular mass(LVmass)],and scanning time.Spearman correlation analysis was used to assess the correlation between the two positioning methods,while Bland-Altman analysis and the intraclass correlation coefficient were applied for consistency evaluation.Results After contrast agent injection,the scanning time for EasyScan automatic positioning was significantly shorter than that for traditional manual positioning[(70.68±22.35)s vs(288.24±77.03)s,P<0.001].Angle measurements showed no statistically significant differences in the angles of the two-chamber view,four-chamber view,and short-axis plane between EasyScan automatic positioning and traditional manual positioning after contrast agent injection(P>0.05).Bland-Altman analysis indicated that all these data points fell within the 95%confidence interval(95%CI).Analysis of short-axis cine images revealed no statistically significant differences in LVEF,LVEDV,LVESV,LVSV,and LVmass between EasyScan automatic positioning and traditional manual positioning after contrast agent injection(P>0.05),and the Spearman correlation coefficients were all higher than 0.78.Conclusion The EasyScan automatic positioning technology demonstrates accuracy and consistency comparable to traditional manual positioning both before and after contrast agent injection,while significantly shortening the scanning time.It can improve the efficiency of clinical CMR examinations and reduce the burden of patient cooperation.

Abstract:Objective To investigate the efficacy of glycine subgingival air polishing in the treatment of patients with type 2 diabetes mellitus(T2DM)and periodontitis and its effects on plaque index(PLI)and the levels of inflammatory factors in gingival crevicular fluid.Methods From June 2023 to December 2024,80 patients with T2DM and periodontitis diagnosed and treated at the Third Hospital of Hebei Medical University were selected and divided into a conventional group and an air polishing group using a simple randomization method(random number table),with 40 cases in each group.The conventional group received traditional ultrasonic scaling combined with manual subgingival scaling and root planing,while the air polishing group received glycine subgingival air polishing.Taking 1 month after initial periodontal therapy as the baseline,bleeding index(BI),probing depth(PD),PLI,and masticatory function(bite force,masticatory efficiency)were compared between the two groups at baseline and at 1,3,and 6 months of maintenance therapy.Glycemic and lipid parameters[glycated hemoglobin(HbA1c),triglyceride(TG),total cholesterol(TC),fasting blood glucose(FPG)]and gingival crevicular fluid inflammatory factors[tumor necrosis factor-α(TNF-α),C-reactive protein(CRP),interleukin-6(IL-6)]were compared at baseline and at 1 month of maintenance therapy.Results Compared with baseline,BI,PD,and PLI in both groups decreased significantly at 1,3,and 6 months of maintenance therapy(P<0.05),and all were significantly lower in the air polishing group than in the conventional group(P<0.05).Compared with baseline,bite force and masticatory efficiency in both groups increased significantly at 1,3,and 6 months of maintenance therapy(P<0.05),and both were significantly higher in the air polishing group than in the conventional group(P<0.05).At 1 month of maintenance therapy,the levels of HbA1c,TG,TC,FPG,TNF-α,CRP,and IL-6 decreased significantly in both groups(P<0.05),and all were lower in the air polishing group than in the conventional group(P<0.05).Conclusion Glycine subgingival air polishing can effectively treat periodontitis in patients with T2DM,improve periodontal health and masticatory function,reduce blood glucose and lipid levels,and alleviate gingival inflammatory response.

Abstract:Objective To investigate the influencing factors of chronic pain after surgery in patients with osteoporotic vertebral compression fractures(OVCF).Methods A retrospective analysis was conducted on the clinical data of 107 OVCF patients who underwent surgery at Huai'an First Hospital Affiliated to Nanjing Medical University from March 2022 to January 2025.The patients were divided into a pain group(n=28)and a pain-free group(n=79)based on the occurrence of chronic pain after surgery.Multivariate logistic stepwise regression analysis was used to analyze the influencing factors of chronic pain after surgery in OVCF patients.Results The visual analogue scale for pain(VAS)score of the 107 OVCF patients at the last postoperative follow-up was 3.77±0.69.During the follow-up period,26.17%(28/107)of the patients developed chronic pain.Univariate analysis showed that the pain group had a significantly higher proportion of patients with fracture segments≥2,bone cement leakage,and fascia injury,as well as a significantly higher bone cement injection volume,compared to the pain-free group.The bone mineral density value,postoperative vertebral height recovery rate,and Cobb angle correction degree were significantly lower in the pain group(P<0.05).Multivariate analysis showed that bone cement leakage(OR=1.740),fascia injury(OR=2.625),increased bone cement injection volume(OR=2.423),low postoperative vertebral height recovery rate(OR=3.762),and low Cobb angle correction degree(OR=2.779)were independent risk factors for chronic pain after OVCF surgery,while a higher bone mineral density value(OR=0.520)was an independent protective factor(all P<0.05).Conclusion Some patients still develop chronic pain after OVCF surgery,which is influenced by multiple factors.Clinical medical staff need to focus on the chronic pain status of patients after surgery and are advised to formulate targeted intervention measures based on the influencing factors to improve postoperative recovery.

Abstract:Ferroptosis,officially named as an iron-dependent programmed cell death pattern in 2012,is characterized by the accumulation of lipid peroxidation and the inactivation of antioxidant defense system,which is fundamentally different from traditional cell death patterns such as apoptosis and necrosis.As one of the most common malignancies of the female reproductive system worldwide,cervical cancer accounts for over 661 000 new cases each year.Patients with intermediate and advanced-stage disease face severe challenges including tumor recurrence,drug resistance,and poor prognosis.Recent studies have established that dysregulated ferroptosis plays a critical role in the development and progression of cervical cancer.Elucidating the related molecular mechanisms offers important targets for novel therapeutic strategies.This article systematically reviews the core regulatory mechanisms of ferroptosis,examines its specific regulatory mechanisms in cervical cancer,and summarizes recent advances,current challenges,and future directions in targeting ferroptosis for cervical cancer treatment.The aim is to provide new insights and a theoretical foundation for the clinical treatment of cervical cancer.

Abstract:Objective To investigate the diagnostic value of transvaginal ultrasound in pernicious placenta previa(PPP)with placenta accreta.Methods A total of 116 patients who underwent cesarean section and were diagnosed with PPP at Baoding No.2 Hospital from April 2017 to July 2022 were selected as the study subjects.All received transvaginal ultrasound to determine placenta accreta.Based on intraoperative findings and postoperative pathological results as the gold standard,patients were divided into a placenta accreta group(n=73)and a non-placenta accreta group(n=43).The diagnostic efficacy of transabdominal ultrasound and transvaginal ultrasound for PPP with placenta accreta was analyzed using receiver operating characteristic(ROC)curves.Results Among the 116 patients,73(62.93%)had placenta accreta,including 28(24.14%)with placenta accreta,33(28.45%)with placenta increta,and 12(10.34%)with placenta percreta;43(37.07%)patients had no placenta accreta.The differences in parity and number of previous cesarean deliveries between the placenta accreta group and the non-placenta accreta group were statistically significant(P<0.05).Using the gold standard as reference,the sensitivity,specificity,accuracy,positive predictive value,and negative predictive value of transabdominal ultrasound for diagnosing PPP with placenta accreta were 84.93%,74.42%,81.03%,84.93%,and 74.42%,respectively(Kappa=0.594).The corresponding values for transvaginal ultrasound were 89.04%,90.70%,89.66%,94.20%,and 82.98%(Kappa=0.782).The specificity of transvaginal ultrasound was higher than that of transabdominal ultrasound(P<0.05).ROC curve analysis showed that the areas under the curve for transabdominal ultrasound and transvaginal ultrasound were 0.797(95%CI:0.712-0.866)and 0.899(95%CI:0.829-0.947),respectively,with a statistically significant difference(Z=3.311,P<0.001).Analysis of ultrasound images from 65 patients with PPP and placenta accreta diagnosed consistently by both transvaginal ultrasound and the gold standard revealed that all cases showed interruption(or disappearance)and thinning of the myometrium at the placental attachment site;78.46%(51/65)showed abnormally abundant blood flow signals behind the placenta;and 81.54%(53/65)showed an ill-defined retroplacental clear space.Conclusion Transvaginal ultrasound has good diagnostic value in patients with PPP and placenta accreta and can be used as an auxiliary method for prenatal diagnosis in clinical practice.

Abstract:Objective To explore the value of ultrasound combined with mammography radiomics in the diagnosis of axillary lymph node metastasis(ALNM)of breast cancer.Methods A total of 160 breast cancer patients admitted to Nanyang Central Hospital from May 2022 to May 2024 were selected.All patients underwent ultrasound examination and mammography radiomics diagnosis.The mammography radiomics model was constructed mainly by extracting radiomics features from each region of interest using the"PyRadiomics"package in the 3D Slicer software,followed by Min-Max normalization.Using pathological examination findings as the gold standard,all patients were divided into a metastasis group(95 cases)and a non-metastasis group(65 cases).The efficacy of ultrasound combined with mammographic radiomics in diagnosing ALNM in breast cancer was analyzed,and ultrasound features and parameters were compared between the metastasis and non-metastasis groups.Results Pathological examination identified 95 positive and 65 negative ALNM cases.Ultrasound detected 94 positive cases,with 13 missed diagnoses and 12 misdiagnoses;mammography radiomics detected 96 positive cases,with 9 missed diagnoses and 10 misdiagnoses;ultrasound combined with mammography radiomics detected 107 positive cases,with 2 missed diagnoses and 14 misdiagnoses.The sensitivity of ultrasound combined with mammography radiomics in diagnosing ALNM in breast cancer was higher than that of ultrasound or mammography radiomics alone(all P<0.05).The proportions of patients with irregular morphology,heterogeneous echogenicity,spiculation sign,mixed blood flow distribution,and Adler blood flow signal grade Ⅱ-Ⅲ in the metastasis group were all higher than those in the non-metastasis group(all P<0.05).The maximum blood flow velocity and resistance index in the metastasis group were also higher than those in the non-metastasis group(all P<0.05).Conclusion Ultrasound combined with mammography radiomics shows improved efficacy in diagnosing ALNM in breast cancer.

Abstract:Objective To analyze the clinical value of systemic immune-inflammation index(SII)in predicting the natural outcome of disease in patients with high-risk human papillomavirus(HPV)infection.Methods A total of 204 patients with high-risk HPV infection who were diagnosed and completed one-year follow-up at Affiliated Baoji Hospital of Xi'an Medical University,from December 2020 to December 2023 were retrospectively included.According to the follow-up outcome,they were divided into the clearance group(142 cases)and the persistent positive group(62 cases).After admission,all patients were tested for platelet count(PLT),neutrophil count(NEUT),lymphocyte count(LYMPH)and other indicators.The neutrophil-to-lymphocyte ratio(NLR)and SII were calculated.General data and blood test-related indicators were analyzed and compared between groups.The value of SII in predicting the natural outcome of high-risk HPV infection was evaluated using receiver operating characteristic(ROC)curve analysis.Multivariate logistic regression was used to analyze independent factors influencing the natural outcome of high-risk HPV infection.Results The proportion of vaginal microecological abnormalities,high-risk HPV viral load,and peripheral blood levels of PLT,NEUT,NLR,and SII in the persistent positive group were higher than those in the clearance group,while the level of LYMPH was lower(P<0.05).ROC curve analysis showed that the areas under the curve(95%CI)of PLT,NLR,and SII for predicting the natural outcome of high-risk HPV infection were 0.733(0.683-0.783),0.848(0.798-0.898),and 0.901(0.851-0.951),respectively.The De-Long test results indicated that the predictive performance of SII was significantly superior to that of PLT(Z=4.654,P<0.05)and NLR(Z=1.468,P<0.05).Multivariate analysis showed that vaginal microecological abnormalities(OR=2.333),elevated high-risk HPV viral load(OR=1.384),and elevated SII(OR=2.724)were independent risk factors for persistent positivity in patients with high-risk HPV infection(P<0.05).Conclusion A significantly elevated SII is an independent risk factor for persistent positivity within one year in patients with high-risk HPV infection and can serve as a comprehensive biomarker for predicting the natural outcome of the disease.Using 1 464.76 as the cutoff value provides the best balanced performance for predicting persistent positivity,offering a specific and quantifiable reference for clinical risk assessment.

Abstract:Objective To investigate the application effect of Enhanced Recovery After Surgery(ERAS)in patients undergoing laparoscopic cholecystectomy in the highfat and highprotein dietary region of Linxia Hui Autonomous Prefecture,Gansu Province.Methods A total of 120 patients with gallstones who underwent laparoscopic cholecystectomy at Linxia County People's Hospital from January 2022 to January 2024 were selected and randomly divided into a control group and a study group,with 60 patients in each group.The control group received conventional perioperative care,while the study group received care based on the ERAS concept tailored to the individual patient.The two groups were compared in terms of operative time,intraoperative blood loss,intraoperative fluid input volume,incidence of intraoperative hypotension,rate of vasoactive drug use,time to first liquid diet intake,time to first ambulation,time to first flatus,postoperative hospital stay,incidence of complications,and visual analogue scale(VAS)pain score at 8 hours after surgery.Results There were no statistically significant differences between the two groups in intraoperative blood loss,fluid input volume,or operative time(P>0.05).The study group had a lower incidence of intraoperative hypotension and a lower rate of vasoactive drug use compared with the control group(P<0.05).The study group showed shorter postoperative hospital stay,earlier time to first flatus,earlier time to first liquid diet intake,and earlier time to first ambulation than the control group(P<0.05),and the VAS score at 8 hours after surgery was lower in the study group(P<0.05).The incidence of complications in the study group(10.00%)was significantly lower than that in the control group(36.67%)(P<0.05).Conclusion Implementing ERAS management for patients undergoing laparoscopic cholecystectomy in the highfat and highprotein dietary region of Linxia Hui Autonomous Prefecture,Gansu Province,can reduce the incidence of intraoperative hypotension and the use of vasoactive drugs,alleviate early postoperative pain,promote the recovery of gastrointestinal function and early ambulation,lower the incidence of complications,and shorten hospital stay,demonstrating favorable clinical application value.

Abstract:Objective To explore the influencing factors of frailty in patients with hematological malignancies undergoing chemotherapy and construct a nomogram model.Methods A total of 326 patients with hematological malignancies undergoing chemotherapy in the First Affiliated Hospital of Air Force Medical University from January 2023 to August 2025 were selected.The patients were divided into a non-frailty group(n=192)and a frailty group(n=134)using the Fried frailty phenotype scale.General data,laboratory indicators(albumin,hemoglobin),and relevant scale scores(activities of daily living,anxiety and depression,nutritional risk,sleep status)were compared between the two groups.Multivariate logistic stepwise regression analysis was used to explore the influencing factors of frailty in these patients.A nomogram was drawn,and the predictive value of the nomogram model was evaluated using the receiver operating characteristic curve.Results The age of patients in the frailty group was significantly higher than that in the non-frailty group,while hemoglobin and albumin levels were significantly lower.The proportions of patients with a monthly income<3 000 yuan,smoking history,≥4 chemotherapy sessions,exercise frequency<3 times per week,impaired activities of daily living,anxiety and depression,sleep disorders,and nutritional risk were all significantly higher in the frailty group than in the non-frailty group,with statistically significant differences(all P<0.05).Multivariate regression analysis showed that older age(OR=1.831,95%CI:1.264-2.652),anxiety and depression(OR=2.277,95%CI:1.434-3.617),and exercise frequency<3 times per week(OR=2.321,95%CI:1.419-3.796)were independent risk factors for frailty in patients with hematological malignancies undergoing chemotherapy,while higher hemoglobin(OR=0.894,95%CI:0.833-0.959)and higher albumin levels(OR=0.807,95%CI:0.715-0.912)were independent protective factors(P<0.05).The nomogram model showed a consistency index of 0.886(95%CI:0.836-0.935).The area under the curve(95%CI)of the nomogram model for predicting frailty was 0.917(0.866-0.965),with a specificity of 0.776 and a sensitivity of 0.881.Conclusion Age,anxiety and depression,exercise frequency,hemoglobin,and albumin are influencing factors of frailty in patients with hematological malignancies undergoing chemotherapy.The nomogram model constructed based on these factors shows good predictive performance.

Abstract:Objective Fuzheng Huayu(FZHY)formula has clear clinical efficacy in the treatment of endometriosis(EM).Previous studies confirmed that its mechanism may be related to the inhibition of angiogenesis.This study focused on this research hotspot to explore the mechanism of FZHY formula in treating EM.Methods The study was divided into two parts.The first part involved bioinformatics analysis.We obtained the drug targets of FZHY formula,the differentially expressed genes in EM,and angiogenesis-related targets,and identified the top 6"EM core targets".The expression matrices of normal and model group samples for these"EM core targets"were obtained from the GSE58178 microarray.A differential group comparison diagram was plotted,a TF-mRNA-miRNA network was constructed,and the core targets of action were screened.The second part consisted of animal experiment validation.An EM rat model was established by allogeneic endometrial transplantation.Model rats were divided into the model group,the Chinese medicine group(FZHY group),and the positive control group,with 6 rats in each group.A sham-operated group(n=6)was set as control.After 14 days of gavage treatment,samples were collected.Serum and endometrial tissues were examined.Enzyme-linked immunosorbent assay was used to detect serum levels of carbohydrate antigen 125(CA125)and vascular endothelial growth factor(VEGF).Reverse transcription quantitative real-time polymerase chain reaction was used to measure the mRNA expression of miR-199a-5p,hypoxia-inducible factor 1α(HIF-1α),VEGF and other related molecules in normal/ectopic endometrial tissues.Results Bioinformatics analysis indicated that in the key process of angiogenesis,FZHY formula may interfere with EM angiogenesis and slow the growth of ectopic lesions by downregulating HIF-1α,VCAM1,STAT3,and SERPINE1,and upregulating MMP2 and PDGFRB.Subsequently,through construction of the"TF-mRNA-miRNA network"and verification by animal experiments,compared with the model group,the Chinese medicine group showed significantly lower serum CA125 and VEGF levels,significantly reduced volume of ectopic lesions,increased expression of miR-199a-5p,and decreased expression of HIF-1α and VEGF[(9.99±1.01)U/mL vs(14.60±0.65)U/mL,P<0.05;(170.04±29.40)pg/mL vs(245.42±36.10)pg/mL,P<0.05;(6.41±2.11)mm³ vs(23.92±4.63)mm³,P<0.05;0.80±0.08 vs 0.02±0.01,P<0.05;1.28±0.06 vs 2.21±0.32,P<0.05;1.29±0.23 vs 3.79±0.80,P<0.05].Conclusion FZHY formula may intervene in the key process of angiogenesis by downregulating the expression of factors such as HIF-1α and VEGF,thereby shrinking EM ectopic lesions and delaying the further progression of EM.

Abstract:Objective To explore the value of serum uric acid to creatinine ratio(SUA/Cr)in evaluating early renal injury in children with Henoch-Schönlein purpura(HSP).Methods A total of 180 children with HSP admitted to Xichang City People's Hospital from January 2021 to January 2025 were retrospectively selected.According to urinalysis results,they were divided into a renal injury group(n=53)and a non-injury group(n=127).Serum uric acid(SUA),creatinine(Cr),and SUA/Cr were compared between the two groups.The predictive value of SUA/Cr for early renal injury in children with HSP was analyzed using ROC curves.General clinical data of the two groups were compared,and multivariate logistic stepwise regression was used to analyze influencing factors for early renal injury in children with HSP.Results SUA,Cr,and SUA/Cr in the injury group were significantly higher than those in the non-injury group[(394.62±31.24)μmol/L vs(329.45±30.51)μmol/L,P<0.001;(72.87±6.52)μmol/L vs(67.55±6.32)μmol/L,P<0.001;5.50±0.73 vs 4.88±0.68,P<0.001].ROC curve analysis showed that the AUC of SUA/Cr for predicting early renal injury in children with HSP was 0.910,which was significantly better than that of SUA alone(0.853)or Cr alone(0.774)(Z=8.547,9.254,P<0.001).The injury group had higher frequencies of recurrent rash,gastrointestinal symptoms,platelet count,D-dimer,fibrinogen,IgA,and urinary microalbumin levels than the non-injury group(P<0.05).Multivariate logistic stepwise regression analysis showed that recurrent rash(OR=1.984,95%CI:1.182-3.328),elevated D-dimer(OR=2.061,95%CI:1.267-3.350),elevated IgA(OR=2.106,95%CI:1.275-3.479),and SUA/Cr≥5.19(OR=3.010,95%CI:1.510-6.001)were independent risk factors for early renal injury in children with HSP(P<0.05).Conclusion Elevated SUA/Cr is significantly associated with early renal injury in children with HSP and shows good predictive performance for early renal injury.

Abstract:Objective To study the effects of different intensities of exercise intervention on blood pressure levels,microvascular reactivity(MR),and left ventricular diastolic function(LVDF)in elderly patients with hypertension.Methods A total of 120 elderly patients with hypertension admitted to Shijiazhuang Hospital of Traditional Chinese Medicine Guanghua Branch from January 2022 to December 2024 were selected and randomly divided into the mild group(n=40),the moderate group(n=40),and the control group(n=40)using a random number table.All three groups received oral levamlodipine maleate tablets.Based on drug treatment,the mild group received mild exercise intervention,and the moderate group received moderate exercise intervention.Systolic blood pressure,diastolic blood pressure,sleep quality,MR indicators[baseline value of microvascular blood perfusion(BV),peak value after skin heating to 44 ℃(VH)],LVDF indicators[mitral A peak(A),mitral E peak(E),and E/A ratio],and adverse reactions during the intervention were compared among the three groups before and after intervention.Results After the intervention,systolic blood pressure,diastolic blood pressure,Pittsburgh Sleep Quality Index(PSQI)score,BV,and A value decreased in the three groups(P<0.05),and the mild and moderate groups were lower than those in the control group(P<0.05),with the moderate group lower than the mild group(P<0.05).After the intervention,VH,E value,and E/A ratio increased in the three groups(P<0.05),and the mild and moderate groups were higher than those in the control group(P<0.05),with the moderate group higher than the mild group(P<0.05).During the intervention period,there were no significant adverse reactions in any of the three groups.Conclusion Under the conditions of this study,compared with mild exercise intervention,moderate exercise intervention not only has a better effect on controlling blood pressure in elderly patients with hypertension,but also can improve their sleep quality and MR,and enhance LVDF.Moreover,both intensities of exercise intervention showed no obvious adverse reactions.

Abstract:Objective To explore the relationship between serum interleukin-6(IL-6)and pregnancy-associated plasma protein A(PAPP-A)levels and fetal distress in parturients with premature rupture of membranes.Methods A retrospective study was conducted on 116 parturients with premature rupture of membranes admitted to Xingtai Central Hospital between March 2023 and January 2025.According to the occurrence of fetal distress,they were divided into a fetal distress group(38 cases)and a non-fetal distress group(78 cases).Clinical data and levels of IL-6 and PAPP-A were compared between the two groups.Logistic regression analysis was used to analyze the related factors influencing fetal distress in parturients with premature rupture of membranes.Results Compared with the non-fetal distress group,the fetal distress group had higher proportions of abnormal fetal position,hypertensive disorder complicating pregnancy,excessive uncoordinated uterine contractions,oligohydramnios,and amniotic fluid contamination,along with a higher IL-6 level and a lower PAPP-A level(P<0.05).Multivariate Logistic regression analysis showed that abnormal fetal position,hypertensive disorder complicating pregnancy,elevated IL-6 level,and decreased PAPP-A level were independent related factors for fetal distress in parturients with premature rupture of membranes(P<0.05).Conclusion Abnormal fetal position,hypertensive disorder complicating pregnancy,elevated IL-6 level,and decreased PAPP-A level are independent related factors for fetal distress in parturients with premature rupture of membranes.

Abstract:Objective To explore the risk factors of catheter-related bloodstream infections(CRBSI)in neonates and predict the risk of CRBSI associated with central venous catheterization.Methods Clinical records of 386 neonates who underwent central venous catheterization at Tangshan Maternal and Child Health Hospital from January 2020 to December 2024 were collected,and the neonates were divided into the CRBSI group(37 cases)and the non-CRBSI group(349 cases)based on whether CRBSI occurred.The t-test and χ2 test were used to compare clinical data between the two groups.Multivariate logistic stepwise regression analyzed influencing factors for neonatal CRBSI,and a nomogram prediction model was constructed.Results Among 386 neonates with central venous catheters,37 developed CRBSI(9.59%),with 42 pathogenic strains isolated(predominantly gram-positive bacteria,57.14%).Risk factors for neonatal CRBSI included:birth weight<1.5 kg(OR=0.523,95%CI:0.381-0.716),low 5-minute Apgar score(OR=0.393,95%CI:0.263-0.586),≥2 puncture attempts(OR=2.401,95%CI:1.629-3.540),catheter indentation time≥2 weeks(OR=2.832,95%CI:1.865-4.299),and elevated procalcitonin levels(OR=2.479,95%CI:1.669-3.684).The nomogram model showed an AUC of 0.896(95%CI:0.844-0.948)for predicting CRBSI,with 67.53%specificity and 92.04%sensitivity.Conclusion The nomogram model based on risk factors for neonatal CRBSI demonstrates good predictive performance and can serve as an important tool for guiding clinical treatment.

Abstract:Objective To preliminarily explore the targets and pathways through which aloin promotes the proliferation of human gingival fibroblasts.Methods A total of 10 cases of gingival tissue trimming from impacted tooth extraction surgeries in the Department of Oral and Maxillofacial Surgery at Hebei Medical University Hospital were randomly selected from January 2024 to January 2025.Human gingival fibroblasts were cultured using the tissue explant adherence method;third-passage cultured cells were selected and placed on glass slides for cell identification.Fibroblasts treated with 40 μmol/L aloin were used as the experimental group,and untreated fibroblasts served as the control group,with three samples in each group.Transcriptome sequencing was performed on both groups to identify differentially expressed genes and conduct KEGG pathway enrichment analysis.Results The sequencing results showed that the expression of ephrin-A1(EFNA1)in the experimental group(0.951 386,0.918 644,0.855 717)was significantly higher than that in the control group(0.207 686,0.274 180,0.238 854),and the difference was statistically significant(P<0.001).KEGG Enrichment analysis of differentially expressed genes revealed that,when ranked by P-value,these genes were significantly enriched in the phosphatidylinositol 3-kinase-protein kinase B pathway.Moreover,the EFNA1 gene is located upstream of this pathway and showed high expression.Conclusion Aloin may promote the proliferation of human gingival fibroblasts by upregulating EFNA1.

Abstract:Objective To investigate the correlation between serum levels of C-C motif chemokine ligand 2(CCL2)and C-X-C motif chemokine ligand 8(CXCL8)and in-stent restenosis(ISR)after percutaneous coronary intervention(PCI)in patients with coronary heart disease(CHD).Methods A total of 202 CHD patients who were admitted to Baoji People's Hospital and underwent PCI from July 2019 to July 2020 were enrolled.Based on the occurrence of ISR within 18 months after PCI,the patients were divided into an ISR group(32 cases)and a non-ISR group(170 cases).Clinical data were collected,and serum levels of CCL2 and CXCL8 were measured.Multivariate logistic regression analysis was used to identify independent influencing factors for ISR after PCI in CHD patients.Relative risk analysis was conducted to determine the impact of high versus low levels of CCL2 and CXCL8 on ISR.Receiver operating characteristic(ROC)curves were plotted to analyze the predictive value of CCL2 and CXCL8 levels for ISR.Results The proportion of diabetes,low-density lipoprotein cholesterol(LDL-C)levels,and serum levels of CCL2 and CXCL8 were significantly higher in the ISR group than in the non-ISR group(P<0.05).Multivariate logistic regression analysis showed that elevated LDL-C(OR=2.305,95%CI:1.202-4.419),elevated CCL2(OR=2.643,95%CI:1.114-6.273),and elevated CXCL8(OR=2.921,95%CI:1.236-6.906)were risk factors for ISR after PCI in CHD patients(P<0.05).Relative risk analysis showed that the risk of ISR in patients with high serum levels of CCL2 and CXCL8 was 2.136 times and 2.614 times higher,respectively,than that in patients with low levels.ROC curve analysis revealed that the area under the curve(AUC)for predicting postoperative ISR in CHD patients was 0.739 for CCL2 and 0.682 for CXCL8.The combined prediction of both markers yielded an AUC of 0.820,which was higher than that of either single indicator(Zcombined vs CCL2=2.031,P=0.042;Zcombined vs CXCL8=2.786,P=0.005).Conclusion Serum levels of CCL2 and CXCL8 were elevated in CHD patients with ISR.Both are independent risk factors for ISR after PCI in CHD patients and have good predictive value for ISR after PCI.