Aloin may Promote the Proliferation of Human Gingival Fibroblasts by Upregulating EFNA1
WU Tianzhen
ZHAO Hua
ZHOU Siwei
LI Na
LIU Jia
YUAN Xuewei
NING Chunliu
ZHANG Xudong
HUANG Wei
Abstract：Objective To preliminarily explore the targets and pathways through which aloin promotes the proliferation of human gingival fibroblasts.Methods A total of 10 cases of gingival tissue trimming from impacted tooth extraction surgeries in the Department of Oral and Maxillofacial Surgery at Hebei Medical University Hospital were randomly selected from January 2024 to January 2025.Human gingival fibroblasts were cultured using the tissue explant adherence method;third-passage cultured cells were selected and placed on glass slides for cell identification.Fibroblasts treated with 40 μmol/L aloin were used as the experimental group,and untreated fibroblasts served as the control group,with three samples in each group.Transcriptome sequencing was performed on both groups to identify differentially expressed genes and conduct KEGG pathway enrichment analysis.Results The sequencing results showed that the expression of ephrin-A1(EFNA1)in the experimental group(0.951 386,0.918 644,0.855 717)was significantly higher than that in the control group(0.207 686,0.274 180,0.238 854),and the difference was statistically significant(P<0.001).KEGG Enrichment analysis of differentially expressed genes revealed that,when ranked by P-value,these genes were significantly enriched in the phosphatidylinositol 3-kinase-protein kinase B pathway.Moreover,the EFNA1 gene is located upstream of this pathway and showed high expression.Conclusion Aloin may promote the proliferation of human gingival fibroblasts by upregulating EFNA1.
Keywords：aloingingival fibroblastscell proliferationephrin-A1
Publication Date：2026-02-20
Online Publishing Date：2026-03-16(First online date of this platform, not the publication date of the document)
Pages：5( 295-299 )