Abstract:Objective To establish the reference interval of the adult red blood cell (RBC) and rele-vant indicators in the plateau region. Methods 279 cases healthy adults in plateau region (average elevation 3500 m) were selected. The levels of RBC, hemoglobin(HGB), mean corpuscular volume(MCV), mean cor-puscular hemoglobin(MCH) and mean corpuscular hemoglobin concentration(MCHC) were detected by SYS-MEX XE-2100 fully automatic blood analyzer. The 95% reference interval of RBC and relevant indicators were evaluated statistically. Results The levels of RBC, HGB, MCH and MCHC of men were all higher than that of women in plateau region, and the differences all had statistical significance(Pall<0.05). The level of RBC in men were (4.6~6.7)×1012/L, women were (4.3~5.9)×1012/L, HGB in men (149.6~220.4) g/L, women were (132.2~186.6) g/L;MCV in men were (82.3~97.4) fl, women were (82.2~95.3) fl;MCH in men were(28.6~36.0) pg, women were(27.0~33.8) pg;MCHC in men were (331.0~389.4) g/L, women were(320.0~375.4) g/L. Conclusion The reference interval of RBC and relevant indicators in the plateau region are established successfully, and the levels of RBC, HGB, MCH and MCHC in men are all higher than that of women.

Abstract:Objective To investigate the expression and clinical significance of autophagy associated gene Atg5 and MAPLC3 in bone marrow mononuclear cells (BMMNC) in patients with acute leukemia (AL). Methods Fluorescent quantitative reverse transcription-polymerase chain reaction (RT-PCR) was employed to detect Atg5 and MAPLC3 mRNA expression levels in BMMNC of 150 cases AL patients and 30 cases of healthy normals. The results were analyzed statistically. Results Compared with the control group, the Atg5 and MAPLC3 mRNA levels in all the risk groups of acute myelogenous leukemia (AML) were significantly re-duced,and the differences all had statistical significance (Pall<0.01). From the low-risk group , risk group to the high-risk group, the Atg5 and MAPLC3 mRNA levels gradually decreased, and the differences between each two groups all had statistical significance (Pall<0.01);Compared with the control group, the Atg5 and MAPLC3 mRNA levels in all the risk groups of acute lymphoblastic leukemia (ALL) were significantly in-creased, and the differences all had statistical significance (Pall<0.01). Atg5 and MAPLC3 mRNA levels in patients with high-risk group was significantly lower than that of standard risk group , the differences all had statistical significance (Pall<0.01);The Atg5 mRNA levels was positively related to the MAPLC3 levels in BMMNC of the control group and AL patients (Pall<0.01). Conclusion The autophagy associated gene Atg5 and MAPLC3 are all expressed, and show positive correlation in AL patients. The autophagic activity in pa-tients with AML is significantly reduced, while in patients with ALL is significantly increased. With increasing risk of both types of leukemia autophagic activity show a significantly decreased trend.

Abstract:Objective To explore the diagnostic value of procalcitonin (PCT) and C-reactive protein (CRP) testing for diagnosing respiratory tract infectious diseases in children. Methods 105 cases children in our hospital from February 2014 to April 2015 were enrolled in this study , which including 39 cases children with bacterial infection, 37 cases children with non-bacterial pathogen infection, and 29 healthy children as the controls. The serum PCT, CRP levels of before and after treatment of children with respiratory tract infec-tious diseases and healthy children were all detected. The results were analyzed statistically. Results There were statistical significance in the differences of PCT, CRP levels among bacterial infection group, non-bacte-rial pathogen infection group and control group (H=61.603, 58.959, Pall<0.01). The serum PCT, CRP levels of bacterial infection group were all higher than that of non-bacterial pathogen infection group and control group, the differences all had statistical significance (ZPCT=-5.950,-6.549, ZCRP=-5.674,-6.591,Pall<0.01). The serum PCT, CRP levels of non-bacterial pathogen infection group were all higher than that of control group, and the differences all had statistical significance (ZPCT=-4.002, ZCRP= -3.708, Pall< 0.01). The serum PCT, CRP levels of bacterial infection group before treatment were all higher than that of after treat-ment, and the differences all had statistical significance (ZPCT=-5.415, ZCRP=-5.428, Pall<0.01). The serum PCT, CRP levels of non-bacterial pathogen infection group before treatment were all higher than that of after treatment, and the differences all had statistical significance (ZPCT=-4.565, ZCRP=-5.137, Pall<0.01). There were no statistical significance in the differences of PCT , CRP levels among after treatment of bacterial infec-tion group, non-bacterial pathogen infection group and control group (H=3.026, 1.531, Pall>0.05). There were statistical significance in the differences of PCT, CRP positive rate among bacterial infection group, non-bacterial pathogen infection group and control group (χ2= 56.85,40.78, Pall< 0.05). The positive rates of PCT, CRP in bacterial infection group and non-bacterial pathogen infection group were all higher than that of control group, and the differences all had statistical significance(Pall<0.05). The positive rates of PCT, CRP in bacterial infection group were all higher than that of non-bacterial pathogen infection group , and the differ-ences all had statistical significance (Pall< 0.05). The area under receiver operating characteristic curve of PCT, CRP and combined detection in diagnosing respiratory tract infectious diseases were 0.928, 0.919 and 0.952, respectively. The sensitivity of PCT, CRP and combined detection were 89.7%, 88.2% and 87.2%, specificity were 84.8%, 87.3%and 95.5%, Youden index were 0.745, 0.755 and 0.827, respectively. Con-clusion The serum PCT and CRP detection can be used for diagnosing the children with respiratory tract in-fection. The joined detection method has relatively high clinical value compared with the two indexes testing lonely.

Abstract:Objective To explore the clinical value of serum cystatin C(CysC) and urinary micro-al-bumin(mALB) detection in the diagnosis of early renal function damage in the children with henoch-schonlein purpura (HSP). Methods 120 cases of HSP children from January 2014 to December 2014 in our hospital department of dermatology were collected. They were divided into normal renal function group (60 cases) and renal dysfunction group(60 cases) according to their renal function. In the same time, 60 healthy children were chosen as control group. The levels of serum CysC and urine mALB of all the children were detected and the re-sults were analyzed statistically. Results There were statistical significance in the differences of serum CysC and urine mALB levels among control group, normal renal function group and renal dysfunction group (F=7.13, F=8.54, Pall<0.05). The serum CysC and urine mALB levels in normal renal function group and renal dysfunction group were all higher than that of control group , and the differences all had statistical significance (Pall<0.05). The serum CysC and urine mALB levels in renal dysfunction group were all higher than that of normal renal function group, and the differences all had statistical significance(Pall<0.05). The positive rates of serum CysC, urine mALB and combine detection in HSP group were all higher than that of control group, and the differences all had statistical significance (Pall<0.05). The positive rates of serum CysC and urine mALB were all lower than that of combine detection, and the differences all had statistical significance (Pall<0.05). Conclusion The serum CysC and urine mALB can be used as the index for the diagnosis of early renal function in the children with HSP.

Abstract:Objective To investigate the clinical application value of heart-fatty acid binding protein (H-FABP) in acute myocardial infarction(AMI) diagnosis. Methods 151 cases AMI patients in our hospital from January 2013 to June 2015 were collected as the AMI group. 68 cases patients with chest pain indications but obviated AMI during the same period were selected as the control group. The levels of H-FABP , cardiac troponin I(cTnI), creatine kinase(CK) and MB isoenzyme of creatine kinase(CK-MB) at 0-3 h, 3-6 h, 6-12 h of all the patients to fall in disease were detected. The results were analyzed statistically. Results The levels of H-FABP, cTnI, CK and CK-MB at 0-3 h, 3-6 h, 6-12 h in AMI group were all higher than that of control group, and the differences all had statistical significance (Pall<0.05). The sensitivity, specificity and area under receiver characteristic operating curve were 78.8%, 89.7%and 0.919, respectively, which were all higher than that of the other three index. There were better correlation among H-FABP and cTnI, CK, CK-MB during each period, and the coefficient correlation r were all higher than 0.8. Conclusion The diagnosis value of H-FABP is better than that of cTnI, CK and CK-MB in AMI diagnosis.