Expression and clinical significance of autophagy associated gene Atg5 and MAPLC3 in bone marrow mononuclear cells in patients with acute leukemia

doi:10.3969/j.issn.1674-7151.2014.03.09

Abstract：Objective To investigate the expression and clinical significance of autophagy associated gene Atg5 and MAPLC3 in bone marrow mononuclear cells (BMMNC) in patients with acute leukemia (AL). Methods Fluorescent quantitative reverse transcription-polymerase chain reaction (RT-PCR) was employed to detect Atg5 and MAPLC3 mRNA expression levels in BMMNC of 150 cases AL patients and 30 cases of healthy normals. The results were analyzed statistically. Results Compared with the control group, the Atg5 and MAPLC3 mRNA levels in all the risk groups of acute myelogenous leukemia (AML) were significantly re-duced,and the differences all had statistical significance (Pall<0.01). From the low-risk group , risk group to the high-risk group, the Atg5 and MAPLC3 mRNA levels gradually decreased, and the differences between each two groups all had statistical significance (Pall<0.01);Compared with the control group, the Atg5 and MAPLC3 mRNA levels in all the risk groups of acute lymphoblastic leukemia (ALL) were significantly in-creased, and the differences all had statistical significance (Pall<0.01). Atg5 and MAPLC3 mRNA levels in patients with high-risk group was significantly lower than that of standard risk group , the differences all had statistical significance (Pall<0.01);The Atg5 mRNA levels was positively related to the MAPLC3 levels in BMMNC of the control group and AL patients (Pall<0.01). Conclusion The autophagy associated gene Atg5 and MAPLC3 are all expressed, and show positive correlation in AL patients. The autophagic activity in pa-tients with AML is significantly reduced, while in patients with ALL is significantly increased. With increasing risk of both types of leukemia autophagic activity show a significantly decreased trend.