Abstract:Objective:To explore the effect of polydatin on the nerve injury in rats with ischemic stroke through regulating the hypoxia-inducible factor-1α(HIF-1α)/vascular endothelial growth factor(VEGF)signaling pathway.Methods:The rat model of ischemic stroke was established by the wire suture method.After modeling,the rats were randomly divided into the model group,the polydatin group(10 mg/kg polydatin),the polydatin+YC-1 group(10 mg/kg polydatin+4 mg/kg YC-1),and the positive drug group(1.0 mg/kg nimodipine),and 10 rats without wire suture insertion were set as the sham operation group.After the intervention,the neurological behaviors of the rats and the infarction area were evaluated.The hematoxylin-eosin(HE)and Nissl staining were used to detect the morphological changes of the brain tissue and the changes in the number of neurons.The expression of microvessel density(CD31)in the brain tissue was determined by immunohistochemistry.The protein expressions of HIF-1α and VEGF in the brain tissue were detected by Western Blot.Results:Compared with the sham operation group,the neurological behavior scores and infarction areas in the model group significantly increased,while the number of neurons,the positive expression of CD31,the protein expressions of HIF-1α and VEGF significantly decreased(P＜0.05).Compared with the model group,the neurological behavior scores and infarction areas in the polydatin group and the positive drug group significantly decreased,while the number of neurons,the positive expression of CD31,the protein expressions of HIF-1α and VEGF significantly increased(P＜0.05).Compared with the polydatin group,the neurological behavior scores and infarction areas in the polydatin+YC-1 group significantly increased,while the number of neurons,the positive expression of CD31,the protein expressions of HIF-1α and VEGF significantly decreased(P＜0.05).Conclusion:Polydatin could reduce the neurological behavior score of rats with ischemic stroke,decrease the infarction area,increase the number of neurons,alleviate the nerve injury,these effects might be related to regulating the HIF-1α/VEGF signaling pathway.

Abstract:Objective:To investigate the protective effect of Shengui Decoction on arrhythmia in rats with acute myocardial ischemia model.Methods:Eighty-four SD rats were randomly divided into the blank control group(NC group),the sham operation group(Sham group),the model group(MI group),the amiodarone group(PC group),the Shengui Decoction high dose group(SHD group),the Shengui Decoction medium dose group(SMD group),and Shengui Decoction low dose group(SLD group).The corresponding dose of the drug was gavaged for 14 days.After ligating the left coronary artery,changes in the standard Ⅱ lead electrocardiogram and J-point voltage were recorded of the rats in each group at 0 minutes(pre-ligation)and at 10,30,60,and 360 minutes post-ligation.The left ventricular systolic pressure(LVSP),left ventricular end-diastolic pressure(LVEDP),heart rate(HR),maximum rate of increase in left ventricular pressure(+dp/dtmax),and maximum rate of decrease in left ventricular pressure(-dp/dtmax)were detected through Hemodynamics.Results:All dosage groups of Shengui Decoction and the PC group the elevation of the J-point at 10,30,60,and 360 minutes post-ligation effectively were reduced,and the J-point elevation significantly lower than that in the MI group(P＜0.01).Compared with the MI group,the SHD group exhibited a more pronounced reduction in J-point elevation at 360 min post-ligation(P＜0.01).The SMD group showed a less significant reduction in J-point elevation compared with that in the PC group at 360 min post-ligation(P＜0.05),while the difference at 10 min post-ligation was not statistically significant with the PC group(P＞0.05).At all time points post-ligation,the reduction in J-point elevation in the SLD group was less pronounced than that in the PC group(P＜0.05).The results of Hemodynamic showed that compared with the MI group,LVEDP significantly in all dosage groups of Shengui Decoction decreaed(P＜0.05 or P＜0.01),while LVSP,+dp/dtmax,-dp/dtmax,and heart rate increasd(P＜0.05 or P＜0.01).Conclusion:All dosage groups of Shengui Decoction reduced the voltage elevation at the J-point in rats with coronary artery ligation,with stable state.Hemodynamic showed that Shengui Decoction could significantly decrease LVEDP,while significantly increased LVSP,+dp/dtmax,-dp/dtmax,and heart rate.The high-dose Shengui Decoction and amiodarone showed better effects.

Abstract:Objective:To evaluate the efficacy and safety of different traditional Chinese medicine injections combined with conventional Western medicine for treatment of gestational hypertension by the network Meta-analysis.Methods:The randomized controlled trials with traditional Chinese medicine injections and conventional Western medicines for treatment of gestational hypertension were searched from PubMed,Cochrane Library,SinoMed,Web of Science,EMbase,China National Knowledge Infrastructure(CNKI),Chinese Biomedical Literature Database,VIP and WanFang data from January 1,2000 to December 31,2023.All researchers independently conducted literature screening,data extraction,and quality assessment.The literatures that met the quality standards were analyzed with Stata 14.0 software,and quality evaluation was performed with ROB2.Results:A total of 38 literatures were included,involving 9 intervention measures,and a total of 3 857 subjects were involved.On the basis of conventional Western medicine,the combination of Mailuoning Injection showed the most significant effect in improving clinical efficacy and reducing the incidence of neonatal asphyxia,Chuanxiongqin Injection was most effective in improving systolic blood pressure,Danshen Chuanxiongqin Injection was most effective in improving diastolic blood pressure,Huangqi Injection was most effective in improving mean arterial pressure and urinary protein,Shenmai Injection was most effective in reducing the incidence of postpartum hemorrhage.Conclusion:The current evidence showed that all 9 types of traditional Chinese medicine injections could improve therapeutic effects,systolic blood pressure,diastolic blood pressure,mean arterial pressure,urine protein and other outcome indicators.Due to limitations in the quality of included literatures,these findings required further validation through multi-center,large-sample,double-blind randomized controlled trials.

Abstract:Objective:To explore Professor Xie Wen's medication rules for treating coronary heart disease based on data mining.Methods:The case data from 111 coronary heart disease patients who visited Professor Xie Wen's outpatient clinic at Hospital of Chengdu University of Traditional Chinese Medicine from January 2023 and January 2024 were selected.The drug formulations from the 111 prescriptions were organized and compiled into an Excel database.Frequency analysis was conducted on the properties,tastes,and meridian tropism of the drugs,visualized using radar charts.Association rules were explored using SPSS Modeler to investigate the combinations of high-frequency drug pairs,with complex network diagrams generated.Cluster analysis was performed using SPSS 26.0,producing a hierarchical clustering tree diagram.Results:A total of 111 prescriptions for treating coronary heart disease from medical institutions were screened and obtained.Among them,19 kinds of drugs had a frequency＞20 times.The four natures were mainly warm,neutral,and cold.The five flavors were mainly pungent,sweet,and bitter.The channel entry were mainly related to the lungs,spleen,and liver.Association rules yielded 10 associated drug groups(with a minimum condition support degree of 0.2 and a minimum rule confidence degree of 0.8),and cluster analysis resulted in 3 clustering patterns.Conclusion:Professor Xie Wen often treated chest Bi and heart pain by regulating congenital constitution,and centering on holistic principles.He adeptly employed authentic Sichuan medicinal herbs,adapting to three factors,while aligning with the principles of the five circuits and six Qi,harmonizing Qi dynamics through simultaneous management of psycho-cardiology and simultaneous treatment of the heart and spleen,regulating the heart spirit.

Abstract:Objective:To observe the effect of Kashgar Berberry Extract on the oxidative stress in atherosclerotic rat models by regulating the peroxisome proliferator-activated receptor-γ(PPARγ)/nuclear factor κB(NF-κB)signaling pathway.Methods:Fifty-five male SD rats of specific pathogen-free(SPF)grade were selected.Ten rats were set as the blank group,while the remaining 45 rats were used to establish the atherosclerosis model,with 40 rats successfully modeling the condition.Forty modeling rats were randomly divided into the model group,the positive drug group,the low-dose Kashgar Berberry Extract group,and the high-dose group,with 10 rats in each group.The blank group and the model group were gavaged with normal saline,the positive drug group was gavaged with atorvastatin,and the low-dose Kashgar Berberry Extract group,and the high-dose Kashgar Berberry Extract group were respectivelygavaged with 60 and 240 mg/(kg·d)of Kashgar Berberry Extract.The histopathological,oxidative stress,serum lipid,endothelin-1(ET-1),nitric oxide(NO),PPARγ mRNA,NF-κB mRNA expressions,and PPARγ/NF-κB protein expression in the aortic tissues of each group of rats were observed.Results:Compared with the blank group,the levels of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),high-density lipoprotein cholesterol(HDL-C),NO,PPARγ mRNA,and PPARγ in the model group decreased(P＜0.05),while malondialdehyde(MDA),reactive oxygen species(ROS),low-density lipoprotein cholesterol(LDL-C),total cholesterol(TC),triglycerides(TG),ET-1,NF-κB mRNA,and NF-κB increased(P＜0.05).Compared with the model group,SOD,GSH-Px,HDL-C,NO,PPARγ mRNA,and PPARγ in the positive drug group,the low-dose Kashgar Berberry Extract group,and the high-dose group significantly increased(P＜0.05),while MDA,ROS,LDL-C,TC,TG,ET-1,NF-κB mRNA,and NF-κB decreased(P＜0.05).Compared with the positive drug group,SOD,GSH-Px,HDL-C,NO,PPARγ mRNA,and PPARγin the low-dose Kashgar Berberry Extract group decreased(P＜0.05),while MDA,ROS,LDL-C,TC,TG,ET-1,NF-κB mRNA,and NF-κB increased.SOD,GSH-Px,LDL-C,TC,TG,NO,PPARγ mRNA,and PPARγ in the high-dose Kashgar Berberry Extract group increased(P＜0.05),while MDA,ROS,HDL-C,ET-1,NF-κB mRNA,and NF-κB decreased(P＜0.05).Conclusion:Kashgar Berberry Extract group could improve the oxidative stress response in atherosclerotic model rats,while its mechanism might be related to regulate the PPARγ/NF-κB signaling pathway.