The Effect of Polydatin on the Nerve Injury in Rats with Ischemic Stroke through Regulating the HIF-1α/VEGF Signaling Pathway
WANG Xiaolong
SANG Juan
LIU Jinliang
TAN Bing
YANG Wei
Abstract：Objective:To explore the effect of polydatin on the nerve injury in rats with ischemic stroke through regulating the hypoxia-inducible factor-1α(HIF-1α)/vascular endothelial growth factor(VEGF)signaling pathway.Methods:The rat model of ischemic stroke was established by the wire suture method.After modeling,the rats were randomly divided into the model group,the polydatin group(10 mg/kg polydatin),the polydatin+YC-1 group(10 mg/kg polydatin+4 mg/kg YC-1),and the positive drug group(1.0 mg/kg nimodipine),and 10 rats without wire suture insertion were set as the sham operation group.After the intervention,the neurological behaviors of the rats and the infarction area were evaluated.The hematoxylin-eosin(HE)and Nissl staining were used to detect the morphological changes of the brain tissue and the changes in the number of neurons.The expression of microvessel density(CD31)in the brain tissue was determined by immunohistochemistry.The protein expressions of HIF-1α and VEGF in the brain tissue were detected by Western Blot.Results:Compared with the sham operation group,the neurological behavior scores and infarction areas in the model group significantly increased,while the number of neurons,the positive expression of CD31,the protein expressions of HIF-1α and VEGF significantly decreased(P＜0.05).Compared with the model group,the neurological behavior scores and infarction areas in the polydatin group and the positive drug group significantly decreased,while the number of neurons,the positive expression of CD31,the protein expressions of HIF-1α and VEGF significantly increased(P＜0.05).Compared with the polydatin group,the neurological behavior scores and infarction areas in the polydatin+YC-1 group significantly increased,while the number of neurons,the positive expression of CD31,the protein expressions of HIF-1α and VEGF significantly decreased(P＜0.05).Conclusion:Polydatin could reduce the neurological behavior score of rats with ischemic stroke,decrease the infarction area,increase the number of neurons,alleviate the nerve injury,these effects might be related to regulating the HIF-1α/VEGF signaling pathway.
Keywords：schemic strokepolydatinhypoxia-inducible factor-1αvascular endothelial growth factornerve injuryexperimental study
Publication Date：2026-02-10
Online Publishing Date：2026-03-09(First online date of this platform, not the publication date of the document)
Pages：6( 365-370 )