Breeding of the New Bitter gourd Cultivar GL 110Abstract:Guangliang 110 was a new variety of oil melon,which was bred by the hybridization of high-generation inbred line YIN-21 as the female parent and high-generation inbred line YIN-32 as the male parent.This variety belonged to the oil gourd type,with strong plant growth vigor.From sowing to first harvest,it took 74-84 days in spring and 50-51 days in autumn.The fruit had a longitudinal diameter of 27-30 cm and a transverse diameter of 6-7 cm,with an average weight of about 450 g.The fruit color was bright and glossy,with a regular shape,a relatively flat shoulder and a blunt tail.In the multi-point trials in Zhuhai,Guangzhou and Zhanjiang in 2023,the average yield in spring was 45 540.00 kg·hm-1,with an increase of 9.7%compared to the control.The average yield in autumn was 36 495.00 kg·hm-1,which was 9.5%higher than that of the control.The variety had good comprehensive disease resistance,and no fusarium wilt occurred in the field test.The disease index of powdery mildew was 9.26,which was better than that of the control Fenglv balsam pear.The results showed that the soluble solids content of Guangliang 110 was 2.7%,the ascorbic acid(vitamin C)content was 54.4 mg·hg-1,and the crude fiber content was 0.8%.On the whole,Guangliang 110 was suitable for being planted in spring and autumn in Guangdong.
Fresh-keeping Effect of Different Concentrations of Ionic Nano-silver Treatment on the Cut Flowers of Dianthus caryophyllusAbstract:The aim of this study was to investigate the fresh-keeping effects of vasing treatments with a novel bactericide,ionic nano-silver(NS),and its combination with citric acid and sucrose,on the cut spray carnations(Dianthus caryophyllus L.'Prince').The results showed that,compared with the control group(using the deionized water as the vase solution),the separate vase treatment of ionized NS,the combination of ionized NS and citric acid,and the combination of ionized NS and citric acid with sucrose could prolong the vase life and improve the ornamental quality of the cut flower to varying degrees,and effectively maintain their fresh weight and water balance of flowering branches.Among them,the vase treatment with 5 mg·L-1 ionic NS+200 mg·L-1 citric acid+3%sucrose had the most prominent effect on the improvement of the ornamental quality of the cut flower,which could obviously promote the opening of the flower.Their florets opened fully,displayed vibrant colors,and emitted a pleasant fragrance.The experimental results demonstrated that the vase treatment with the combination solution of ionic NS,citric acid and sucrose with appropriate concentration had good preservation effect and application prospect on Dianthus caryophyllus L.'Prince'.
Review on Remediation Techniques of Soil Heavy Metal PollutionAbstract:The remediation technologies for soil heavy metal pollution were sorted out and summarized,which provided a reference for the subsequent treatment of soil heavy metal pollution.By using the form of literature review,the soil heavy metal remediation technologies were summarized,and then the problems and prospects were put forward.At present,the main application of soil heavy metal remediation technology was bioremediation,chemical remediation,physical remediation and combined remediation technology.These repair techniques could achieve good repair results in repairing the heavy metal pollution in soil.However,there were also some limitations.When carrying out the remediation of heavy metal pollution in soil,it was necessary to select the appropriate remediation technologies according to the specific conditions of different soils.The research conclusions included strengthening the follow-up research of remediation technology,controlling the pollution from the root causes and preventing the secondary pollution;considering the soil condition of the contaminated land,scientifically applying the soil heavy metal remediation technology;and flexibly selecting the combined repair technology according to the local conditions.Through the cooperation of different repair technologies,other problems arising in the repair process could be solved.
Effect of Chemical Fertilizer Reduction Combined with the Application of Biological Organic Fertilizers on the Yield,Quality,and Soil Enzyme Activity of Water SpinachAbstract:In order to explore the effects of reducing the chemical fertilizers combined with the application of bio-organic fertilizers on the yield,quality,and soil enzyme activity of water spinach,the field experiments were conducted with four fertilization treatments(T1:20%reduction in chemical fertilizers+bio-organic fertilizers,T2:20%reduction in chemical fertilizers+organic fertilizers without added functional bacteria,CK1:single application of chemical fertilizers,CK2:no fertilization),in order to analyze the effects of different treatments on the growth,yield and quality of water spinach,and the soil enzyme activity.The results showed that the T1 treatment group had the best plant height,root length,leaf length,leaf width,yield,quality,and soil enzyme activity for water spinach.Among them,the yield of T1 increased by 12.6%compared to CK1;The contents of Vc,soluble sugar and soluble protein increased by 27.4%,25.0%,and 19.0%,respectively,while the nitrate content decreased by 31.0%.The soil enzyme activities(alkaline phosphatase,catalase,urease,sucrase,protease)increased by 17.79%,22.61%,19.26%,35.43%,and 42.98%,respectively,with significant differences observed.The results showed that a 20%reduction in chemical fertilizers combined with the application of biological organic fertilizers could effectively improve the yield,quality,and soil enzyme activity of water spinach.
Special Subject Planning as a Win-Win Practice for Enhancing the Quality and Cultivating the Editorial Talents in Agricultural Science and Technology JournalsAbstract:The special subject planning is an important manifestation of science and technology journals to give full play to their subjective initiative and carry out the creative work,which can not only effectively solve the problem of homogenization of journals,but also create a distinctive logo of journals and enhance the academic influence and attention of journals.By sorting out the special subject planning practice of Journal of Southern Agriculture,it concluded that the special subject planning could achieve the win-win situation between the quality improvement of agricultural sci-tech journals and the cultivation of editorial talents.The special subject planning could lead the frontier of disciplines to optimize the structure of subject selection;gather the high-quality manuscript sources to enhance the core competitiveness of content;create the characteristic brands to enhance the recognition of journals;concentrate the exposure and exhibition to enhance the communication and influence,thereby ultimately driving the improvement of the quality of agricultural science and technology journals.(2)The special subject planning could deepen the professional competence and sensitivity to academic trends,improve the core professional ability of editors in an all-round way,and cultivate the innovative thinking and the application ability of new technologies,and strengthen the professional self-confidence and sense of acquisition of editors,thereby forging the high-quality editors of sci-tech journals.In addition,although the special subject planning could effectively focus on the frontier,lead the academic,and enhance the influence of journals,it should be combined with its own characteristics and location advantages,which was consistent with the positioning and development goals of journals.At the same time,the long-term special subject planning should be formulated,and the scientific assessment and incentive mechanism should be established to ensure the sustainability of special subject planning.
Analysis on the Biological Characteristics and Pathogenicity of Ustilaginoidea virens in FujianAbstract:In order to clarify the biological characteristics and pathogenicity level of Ustilaginoidea virens in Fujian,the colony color,mycelial growth rate,sporulation ability and pathogenicity of 74 strains of U.virens collected from 6 regions of Fujian Province were determined by using the methods of conventional biological culture and artificial injection inoculation.The results showed that four phenotypes,namely white,yellowish green,green and dark green,appeared on the back of the colonies of the tested 74 strains of U.virens,with the frequencies of 37.84%,35.14%,17.57%and 11.45%,respectively.The occurrence frequencies of strains with a fast mycelial growth rate(greater than 2 mm·d-1)and strong sporulation ability(sporulation quantity greater than 3×106 cells·mL-1)were 66.21%and 64.86%,respectively.The occurrence frequencies of strains with the disease index ranged from 25 to 50 and greater than 50 were 56.76%and 20.27%,respectively.The mycelial growth rate of U.virens was negatively correlated with the pathogenicity at a low degree(R=-0.305),and the sporulation ability was positively correlated with the pathogenicity at a moderate degree(R=0.509).In Fujian region,there was obvious differentiation in the biological characteristics of U.virens,among which the strains with fast mycelial growth rate,strong sporulation ability and moderate pathogenicity were dominant.
Analysis of Fungal Community Characteristics in Roots and Rhizosphere Soil Between Healthy Tomato Plants and Plants Infected by Root-knot NematodesAbstract:In order to explore the new bio-control resources of root-knot nematodes and develop the new bio-control strategies for nematodes,based on the fungal ITS amplicon sequencing technology,the differences of fungal community characteristics between the root system(healthy root HR,non-root knot part of diseased root INR,root knot part of diseased root IRK)and the rhizosphere soil(healthy soil HRS,diseased root soil IRS)of tomato healthy plants and the plants infected by root-knot nematode were compared.The results showed that the healthy root(HR)of tomato was dominated by Glomeromycota,while at the order level,Glomerale was dominant.The diseased roots(INR,IRK)were dominated by Ascomycota.At the order level,the abundance of Glomerales fungi decreased,while the abundance of Hypocreales,Saccharomycetales,Eurotiales and Pleosporales fungi increased.The dominant phylum in the healthy rhizosphere soil(HRS)was Rozellomycota,and the dominant phylum in the diseased rhizosphere soil(IRS)was Ascomycota,among which Hypocreales was the dominant bacteria.The α diversity of fungal community showed that after the root-knot nematode infection,the richness(Chao1 index)of fungal communities in the diseased roots(INR/IRK)decreased,while the richness(Chao1 index)and diversity(Shannon index)of fungal communities in the rhizosphere soil(IRS)were higher than those in the healthy soil(HRS).The β diversity of fungal community showed that the fungal community structure of the five samples was significantly separated.The study showed that the infection of root-knot nematodes will transform the fungi of tomato roots from a symbiotic type dominated by Glomeromycota to an interactive type dominated by Ascomycota,which may be related to the synergistic pathogenicity of specific fungal groups or the recruitment of root-knot nematode antagonistic groups by plants.
Evaluation of the Predatory Potential of Arma chinensis to the Larvae of Pieris rapaeAbstract:In order to clarify the characteristics of predatory behavior of Arma chinensis on the larvae of Pieris rapae and evaluate the predatory capacity and control potential of A.chinensis,the predatory behavior and the predatory capacity of adults and nymphs of A.chinensis to the larvae of P.rapae were studied by means of indoor observation method combined with the method of HollingⅡdisc equation fitting.The results showed that the predatory behavior of A.chinensis on the larvae of P.rapae could be divided into 4 kind of behaviors:searching,piercing-sucking,waiting and feeding.A.chinensis first fed on the larvae of P.rapae by piercing-sucking through its chest,or by piercing through multiple positions on its body.After the larvae of P.rapae were controlled,multiple A.chinensis would gather to feed.The predatory functional response of 4th and 5th instar nymphs,male and female adults of A.chinensis to 4th instar larvae of P.rapae was in accordance with the Holling Ⅱdisc equation model.The predation amount of A.chinensis increased with the increase of the density of the larvae of P.rapae,while the searching efficiency decreased with the increase of the density of the larvae of P.rapae.The instantaneous attack rate to the 4th instar larvae of P.rapae was as followed:4th instar nymphs(0.927)>5th instar nymphs(0.906)>female adults(0.894)>male adults(0.877).The maximum daily predation of female adults of A.chinensis was 7.645 individuals.The predation of the 5th instar nymphs and male adults of A.chinensis was equivalent,and their searching efficiency was similar.This study showed that both the adults and 5th instar nymphs of A.chinensis had better predation ability on the larvae of P.rapae.Therefore,during the prevention and control,a large number of A.chinensis could be released in the early stage of P.rapae occurrence,thus to reduce the population of P.rapae and minimize their harm to cruciferous vegetables.
Enrichment and Isolation Identification of Ammonia-Nitrogen Degrading Bacteria in Black Soldier Fly FrassAbstract:The aim of this study was to screen the ammonia-nitrogen degrading bacteria adapted to the breeding environment of Hermtia illucens L.and inhibit the ammonia emission during the breeding process of H.illucens L.By using the ammonium sulfate as the sole nitrogen source,the bacteria in H.illucens frass were subjected to enrichment culture,followed by isolation and purification,and the ammonia-nitrogen degradation rate of the strain was also determined,in order to select the ammonia-nitrogen-degrading bacteria.The composition changes of the bacterial community in H.illucens frass during the enrichment culture process were further analyzed through the high-throughput sequencing.As the results showed,After the first enrichment culture,four strains were isolated from H.illucens frass,among which the strains of FJAT-48032 and FJAT-48033 showed 99.93%16SrDNA sequence similarity to Providencia rettgeri and Providencia vermicola,respectively;and the strains of FJAT-48034 and FJAT-48035 both exhibited 99.93%16SrDNA sequence similarity to Pseudomonas aeruginosa.After six consecutive enrichment cultures,the strains of FJAT-48041 and FJAT-48042 were isolated from black soldier fly frass,with 100.00%16SrDNA sequence similarity to Lysinibacillus macroides.The strains with the strongest ammonia-nitrogen degradation activity were FJAT-48041 and FJAT-48042,which achieved an ammonia-nitrogen degradation rate of 73.03%-76.97%within 96 hours.The relative abundance of Enterobacteriaceae and Alcaligenaceae in the sample bacterial suspensions increased after the enrichment culture.The abundance of Enterobacteriaceae dramatically rose from 0.03%before culture to 42.33%after the first enrichment culture,and further increased to 74.85%after three consecutive enrichment cultures.However,after the continuous enrichment culture,the abundance of Enterobacteriaceae decreased,falling to 48.52%after the sixth enrichment culture,which was still significantly higher than that before enrichment culture.This study could provide a reference for the microbial treatment of ammonia emissions in the breeding of H.illucens.
Establishment of Rice GW3p6 Gene Based on the Conventional Melting Temperature AnalysisAbstract:Grain shape is a key trait controlling the rice yield and quality,a 15-base sequence(5'-tccttcaccaagga-3')in the boundary region of the last exon of the GW3p6 gene was mutated to a 13-base sequence(5'-agtatatatacat-3'),leading to the alterations in the intron splicing sites,which in turn made the grain shape of rice slender,thereby improving the rice quality and yield.In order to promote the application of the GW3p6 gene in rice breeding for high yield and quality,a high-efficiency and low-cost functional molecular marker of GW3p6 gene based on the melting temperature(Tm)detection was developed.By designing primers flanking the GW3p6 mutation site and utilizing the melting temperature analysis function of the QuantStudio™3 quantitative PCR instrument,the GW3p6 genotypes of 96 rice samples were detected.The detecting results showed that the two homozygous alleles exhibited distinct melting temperatures of 74.33℃and 71.16℃,respectively,while the heterozygous genotype displayed two non-overlapping melting peaks,enabling accurate identification of the above three different genotypes.The development of the functional marker will promote the application of the GW3p6 gene in rice breeding.
Genetic Diversity of Mytilus coruscus Populations in Pingtan,Fujian and Zhoushan,Zhejiang Based on the COI Gene and H3 GeneAbstract:In order to explore the impact of cross-regional introduction on the genetic diversity of Mytilus coruscus populations and the degree of genetic differentiation between regions,thereby providing a scientific basis for the protection of its germplasm resources,the samples of Mytilus coruscus were collected from the reefs of Pingtan(Fujian)and Zhoushan Islands(Zhejiang).Then,the genetic evolution analysis was conducted by using the mitochondrial COI gene and nuclear H3 gene.The results showed that there was no difference in the H3 gene of Mytilus coruscus between the two populations from Pingtan(Fujian)and Zhoushan(Zhejiang),so it was not suitable for the study of genetic structure.The COI gene sequences of Mytilus coruscus from the two populations were 661 bp in length,with an A+T content of 60.0%.They exhibited the genetic variations suitable for analysis,conform to the characteristics of mitochondrial molecular markers,and the sequences were stable and reliable.Therefore,the COI gene was chosen for the subsequent analysis of the population genetic diversity of Mytilus coruscus.A total of 32 polymorphic sites and 19 haplotypes were detected in the COI gene sequences of the two Mytilus coruscus populations.The overall haplotype diversity(Hd)was 0.9373,and the nucleotide diversity(Pi)was 0.00682.Among them,the genetic diversity of the Pingtan(Fujian)population(Hd=0.9579,Pi=0.00848)was higher than that of the Zhoushan(Zhejiang)population(Hd=0.8421,Pi=0.00404).The genetic differentiation coefficient(Fst)between the two Mytilus coruscus populations was 0.00196(P<0.01).The AMOVA analysis showed that 92.07%of the genetic variation in the two Mytilus coruscus populations came from within the populations.The haplotype network and UPGMA tree of the two Mytilus coruscus populations showed that only 2 of the 19 haplotypes were shared,and there was an obvious geographical branching structure.The results provided a basis for the protection and sustainable utilization of Mytilus coruscus germplasm resources.
Effects of Organic Fertilizer Combined with Oyster Shell Soil Conditioner on Physicochemical Properties of Acidified Soil and Cauliflower YieldAbstract:In order to explore the optimization technology for improving the acidified soils in Zhangpu County,a field experiment was conducted on yellow mud sandy soil,which involved applying the organic fertilizer(9000 kg·hm-2)and oyster shell soil conditioners at different rates(0,1500,3000,and 4500 kg·hm-2)based on the conventional fertilization for cauliflower.The results showed that,compared with the conventional fertilization,the treatments of combining organic fertilizer with oyster shell soil conditioners at different rates increased the soil pH by 0.12-1.09 units,the soil exchangeable calcium by 2.4-34.7 mg·kg-1,and the soil available potassium by 4.6-14.6 mg·kg-1 in the acidified soil with an initial pH of 4.8.Among them,the treatment with 4500 kg·hm-2 of oyster shell soil conditioner showed the most significant effect,followed by the treatment with 3000 kg·hm-2.The acceptance of actual yield indicated that,in comparison with the conventional fertilization,the application of organic fertilizer combined with oyster shell soil conditioners at different rates increased the yield of cauliflower by 6.7%-13.9%,and the treatment with 3000 kg·hm-2 of oyster shell soil conditioner showed the most significant yield-increasing effect.By considering comprehensively the effects of acidification improvement,soil fertility enhancement,yield increase,and income growth,the optimal technical measure for improving the acidification and enhancing the fertility of acidified soils in this region was to apply 9000 kg·hm-2 of organic fertilizer and 3000 kg·hm-2 of oyster shell soil conditioner on the basis of conventional fertilization.
Effects of Continuous Cropping on the Yield,Quality,and Soil Microbial Diversity of Pseudostellaria heterophyllaAbstract:In order to explore the changes of soil microbial diversity in continuous cropping fields of Pseudostellaria heterophylla,a field experiment was conducted to investigate the effects of continuous cropping land treatment on the yield and quality of Pseudostellaria heterophylla,soil microbial diversity and structural distribution by using the first stubble field treatment(CK)as the control group.The results showed that compared with the first stubble land,the fresh weight yield,polysaccharide content,and saponin content of Pseudostellaria heterophylla in the continuous cropping fields were all reduced,with the fresh weight yield decreasing by 76.85%,the polysaccharide content decreasing by 3.16%,and the saponin content decreasing by 75.00%.The number of unique or shared fungal OTUs in the rhizosphere soil microorganisms of Pseudostellaria heterophylla was different between the continuous cropping land and the first stubble land.The number of OUT of unique fungi and bacteria in the rhizosphere soil of continuous cropping land was lower than that of the first stubble land(CK).The composition of fungal community in the rhizosphere soil of continuous cropping land exhibited greater difference,while the bacterial community composition showed higher similarity.In contrast,the composition of bacterial community in the rhizosphere soil of first stubble land(CK)exhibited greater difference,while the fungal community composition showed higher similarity.In the continuous cropping soil treatment,the significantly enriched root-zone soil fungal communities included Chytridiomycota,Mortierellomycota,and Mortierella,etc.In the first-stubble land treatment,the significantly enriched root-zone soil fungal communities mainly were mainly Mucoromycota,etc.In the continuous cropping soil treatment,the significantly enriched root-zone soil bacterial communities included Chloroflexi,Acidobacteria,and Rhodanobacter,etc.As for the first-stubble land treatment,the significantly enriched root-zone soil bacterial communities included Firmicutes,Cyanobacteria,Ochrobactrum,and Acinetobacter,etc.This study preliminarily explored the differences in the diversity and distribution characteristics of fungal and bacterial communities in the rhizosphere soil of continuous cropping land and the first stubble land,which provided a reference for solving the continuous cropping obstacle of Pseudostellaria heterophylla.
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Analysis on the Current Situation and Countermeasures of Rural Collective Economic Development in Coastal Agricultural CountiesAbstract:This paper aimed to systematically analyze the development practice of rural collective economy in Zhangpu County,Fujian Province,and provide useful reference for the high-quality development of rural collective economy.Zhangpu County of Fujian Province had the characteristics of both mountain and sea,and the regional development was obviously different.By taking Zhangpu County as the example,the development status,main income sources and existing problems of rural collective economy in Zhangpu County were combed out through the field investigation and data analysis in this paper.The results showed that:the rural collective economy in Zhangpu County had the characteristics of relatively high income,large regional gap and overall stable operation of income and expenditure,and the source of income showed a trend of diversification.However,there were still shortcomings in terms of development foundation,management talents and support policies.Based on this,some countermeasures were put forward including broadening the development path of village collective economy,strengthening the policy support,strengthening the talent support and improving the supervision and management of collective assets.
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Study on Soil Acidification and Contents of Rare Earth Elements and Aluminum in Soil-Tea System of Typical Tea Gardens in FujianAbstract:In order to explore the soil acidification and the changes of aluminum(Al)and rare earth elements(REEs)contents in the soil-tea system of tea gardens in Fujian Province,the typical tea areas of Anxi(38),Jian'ou(12)and Shouning(10)in Fujian were taken as the research objects.The samples of tea garden soil(tea planting soil),soil without tea planting near the tea garden(natural soil),as well as old leaves,mature leaves and tender leaves of tea were collected according to the years of tea planting,and the physical and chemical properties of soil and the contents of Al and REEs in the samples were analyzed.The results showed that:the content of available phosphorus and available potassium in the tea planting soil of typical tea plantation in Fujian was significantly higher than that in natural soil,but the pH decreased significantly.Among them,the proportion of tea planting soil samples with pH lower than 4.5 was as high as 87.50%.With the increase of tea planting years,the acidification of tea planting soil increased,and the content of exchangeable Al also increased significantly,while the content of exchangeable calcium and magnesium decreased significantly.The average content of total REEs in typical tea garden soils in Fujian was 247.632 mg·kg-1,which was higher than the average level of soil in the world and China.Among them,the contents of cerium(Ce)and lanthanum(La)accounted for 62%of the total REEs content.The total REEs content in soil was significantly positively correlated with pH and significantly negatively correlated with soil alkaline hydrolyzable nitrogen contents.In tea leaves,the Al content increased significantly with the increase of leaf age.The Al content in old leaves was 15.3~28.5 times higher than that in tender leaves.The Al content in old leaves,mature leaves and tender leaves of Anxi tea garden was higher than that in Jian'ou and Shouning tea gardens.The absorption of total REEs by tea was mainly dominated by light REEs,which accounted for more than 70%of the total REE content.The total REEs content in old leaves was 10~15 times higher than that in young leaves.The total REEs content in Shouning and Anxi tea leaves was significantly higher than that in Jian'ou tea leaves,and the light REEs content in Shouning tea leaves was also significantly higher than that in Jian'ou tea leaves.This study revealed the relationship between soil acidification and Al accumulation in Fujian tea gardens,as well as the enrichment characteristics of Al and REEs in tea leaves,providing a scientific basis for the soil management and tea quality and safety in tea gardens.
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Screening and Analysis of New Rice Varieties with High Amylose ContentAbstract:In order to screen out the new rice varieties of special rice suitable for rice noodle processing to meet the increasing demand for special rice in the industry of rice flour processing.In 2024,11 new rice varieties with high grain amylose content,including Lanyou 151,Lanyou 7954,and Chengyou 7954,were selected.By taking Yiyou 673 as the control variety(CK),a variety screening experiment was carried out in Nanping Modern Agricultural Breeding Base of Fujian Academy of Agricultural Sciences.Then,the growth period,main agronomic traits,yield,rice blast resistance and rice quality of the tested varieties were systematically compared and analyzed.The results showed that:compared with Yiyou 673(CK),the yield of the two rice varieties,Lanyou 7954 and Chengyou 7954,was significantly increased by 7.23%and 6.38%,respectively.They showed moderate resistance to rice blast and high grain amylose content(26.7%and 26.3%,respectively),exhibiting exceptional comprehensive traits.They were ideal rice varieties specifically developed for the processing of rice noodles.The yield of Lanyou 151,Xuanliangyou Waiqi and Chengyou 3219 was higher than that of Yiyou 673(CK),which increased by 4.26%,2.13%and 0.43%,respectively.They showed strong resistance to rice blast and high grain amylose content(27.2%,26.0%,and 26.2%,respectively),with good comprehensive traits,which could be used as the important candidate varieties for special rice used in the processing of rice noodles.
Study on the Origin Traceability of Wuyi Rock Tea Based on Mineral Element Fingerprinting TechnologyAbstract:Due to the consistency of appearance and processing,the place of origin of Wuyi rock tea can not be identified by its appearance characteristics.The elemental composition of tea leaves is different due to the different environment of producing area,forming the unique elemental fingerprint of tea producing area.By measuring the contents of various elements in different producing areas of Wuyi rock tea,the origin discrimination model was established by means of stoichiometric statistical method,which provided technical reference for the origin discrimination of Wuyi rock tea.A total of 55 tea green samples were collected from three main producing areas of Wuyi rock tea.The contents of mineral elements were determined by inductively coupled plasma optical emission spectrometry(ICP-OES)and inductively coupled plasma mass spectrometry(ICP-MS),and the data were analyzed by using the principal component analysis.At the same time,two discriminant methods,including the orthogonal partial least squares discriminant analysis(OPLS-DA)and Fisher linear discriminant analysis(FLDA),were used to discriminate the producing areas of Wuyi rock tea.The results showed that:the elements in the tea samples from the three main producing areas of Wuyi rock tea showed different characteristics.The content of the elements in the samples was tested by Bartlett's sphericity test.The Kaiser Meyer-Olkin(KMO)value was 0.650,and KMO>0.5 indicated that the grouping clustering effect was better.Through the principal component analysis,five principal components were successfully extracted,and their eigenvalues were more than 1,and the cumulative variance contribution rate was 71.560%.The cumulative interpretation ability parameters of the orthogonal partial least squares discriminant model were R2X=0.652,R2Y=0.752,and the predictive ability parameter Q2 was 0.590,all exceeding 0.5.The correct discrimination rate and cross-validation rate of the Fisher linear discriminant model were more than 90.9%.Both models could accurately discriminate the tea green samples collected from three different producing areas.This study demonstrated that the mineral elemental fingerprints of Wuyi rock tea samples from three different producing areas,combined with stoichiometric statistical methods,could effectively identify their origin,providing a theoretical basis for the protection of place of origin of Wuyi rock tea.
Effect of Emamectin Benzoate on the Growth,Development and Consumption Rate of Ectropis grisescens WarrenAbstract:This study aimed to investigate the effects of emamectin benzoate on the growth,development and consumption rate of Ectropis grisescens,in order to provide data support for its application in the controlling of Ectropis grisescens.By constructing the age-stage two-sex life table,the effects of feeding on the second leaf under tea shoot buds treated with 0.0091 mg·L-1 emamectin benzoate on the developmental duration,survival rate,fecundity,adult longevity and consumption rate of E.grisescens were analyzed with the water treatment as the control group.The results showed that:compared with the water control group,the survival rate(70.00%),total consumption rate(3211.58 mm2),intrinsic rate of increase(r=0.1241 d-1),net reproductive rate(R0=71.14 offspring/individual)and fecundity(187.21 offspring/individual)of E.grisescens larvae in the treatment group of emamectin benzoate were significantly decreased(the values in the water control group were 98.00%,4529.36 mm2,0.1325 d-1,and 82.18 offspring/individual,and 205.45 offspring/individual,respectively).The larval stage(30.40 d),mean generation time(T=34.36 d)and total pre-oviposition period(TPOP=32.37 d)were significantly prolonged(the values in the water control group were 29.14 d,33.28 d and 31.55 d,respectively).The study showed that the treatment of 0.0091 mg/L emamectin benzoate significantly inhibited the consumption rate,developmental duration,and intrinsic rate of increase of E.grisescens,which demonstrated a certain control effect.
Introduction and Screening of High-quality Ratooning Rice Varieties Suitable for Southern HenanAbstract:In order to screen out the ratooning rice varieties with high yield,high quality and high resistance,which were suitable for being popularized and planted in southern Henan,Liangyou 6326(a high-quality rice variety widely cultivated in Jianghuai region since 2021)was taken as the control variety(CK),and fifteen high-quality varieties of ratooning rice were introduced to carry out the screening test.Then,the growth period,yield and main agronomic traits of each rice variety were investigated.The results showed that:among the tested varieties,the seven varieties including Lvyinzhan,Shenliangyou 867,Meiliangyou Huangzhan,Keliangyou No.1,Longjingyou 1212,Taoyouxiangzhan and Liangyou 6326(CK)had a total yield of more than 12 000 kg·hm-2 in double seasons.Among them,the total yield of Taoyouxiangzhan and Longjingyou 1212 in the two seasons reached 13 567.87 kg·hm-2 and 13,122.23 kg·hm-2,respectively,which were 4.3%and 0.9%higher than that of Liangyou 6326(CK),showing good yield.These two cultivars also demonstrated excellent grain quality and shorter growth duration in both the first-crop and ratoon-crop seasons.Therefore,they could effectively avoid the safety impact caused by the cold-dew wind,and had the value of being the leading popularized variety of ratooning rice in the southern Henan rice area.
Effects of Different Weeding Methods on Runoff Loss of Soil Nitrogen and Phosphorus in Torreya grandis PlantationsAbstract:Mastering the effects of weeding measures on the surface runoff and nitrogen(N)and phosphorus(P)loss in Torreya grandis plantations could provide a certain basis for the management of Torreya grandis forest land.Four runoff plot treatments were set up in Torreya grandis plantations at Dongyang Forestry General Farm,Zhejiang Province,including the young forest-weeding,young forest-control,middle-aged forest-weeding,and middle-aged forest-control.The surface runoff volume in 2024 was comprehensively monitored,the concentrations of different forms of N and P in runoff water were analyzed,and N and P loss amounts were calculated.The results showed that the order of surface runoff volume and N/P loss amounts was as follows:young forest-weeding>young forest-control>middle-aged forest-weeding>middle-aged forest-control.The average annual surface runoff in young Torreya grandis forest was about 3.4 times that in middle-aged forest.After implementing the weeding measures,the annual N and P loss amounts in young Torreya grandis forests increased by 4.6%and 5.9%,respectively,compared with the control group,while those in the middle-aged Torreya grandis forests increased by 9.7%and 3.6%,respectively,compared with the control group.The average nitrogen and phosphorus loss amounts in young forests were 3.5 and 3.6 times those in middle-aged forests,respectively.In conclusion,the surface runoff and N/P loss amounts in young Torreya grandis plantations were significantly higher than those in the middle-aged plantations.The mechanical weeding had little impact on soil N and P loss in Torreya grandis plantations,which could be promoted and applied in production in the future.