Abstract:As an important renewable marine biological resource,the high-value utilization of seaweed is a hot spot in current research and industry.The main nutritional components(e.g.,proteins,lipids,polysaccharides,minerals,vitamins)and biological activities of seaweed were reviewed in this paper,with a focus on its processing technology and the latest progress and application prospects in many fields.In the food industry,the seaweed has been developed into a variety of new healthy foods,and its extracts could be used as the natural additives.In the field of health industry,its active ingredients showed great potential in the development of functional foods and drugs,which was of great significance for the prevention of chronic diseases.In the cosmetics industry,the seaweed extracts were the important natural raw material for skin care.In addition,the seaweed-based biomaterials also showed unique advantages in environmentally degradable materials and high-end biomedical materials.Although the prospect was broad,the high-value utilization of seaweed still faced multiple challenges such as the resource shortage,immature technology and market competition.Therefore,the future research should focus on the key directions such as the efficient utilization of resources,advances in processing technology and intelligent upgrading,in-depth analysis of activity mechanism and industrial transformation,so as to systematically promote the high-value development and sustainable utilization of seaweed resources.

Abstract:Based on the field investigation of farmland ecosystem in Songyang County,Zhejiang Province,the species composition,altitude distribution pattern and habitat preference of invasive weeds were studied.The results showed that a total of 10 species of invasive weeds were recorded,belonging to 7 families,with Compositae plants being the most represented family(4 species),accounting for 40.0%of the total species.The invasion intensity(average coverage)exhibited a declining trend with the increase of altitude.The average coverage in the low-altitude group(＜150 m)was 64.5%±22.0%(n=9),which was higher than that in the mid-altitude group(150-250 m,48.0%±15.2%,n=8)and that in the high-altitude group(＞250 m,34.7%±9.0%,n=3),with the Kruskal-Wallis test showing the marginal significance(H=5.95,P=0.051).The Chi-square test showed that the distribution of species was significantly correlated with altitude(χ2=34.49,P=0.011).Ageratum conyzoides was exclusively distributed in the high-altitude areas,while Pontederia crassipes Mart.was concentrated in the mid-altitude areas.The results provided a reference for monitoring,early warning and classified prevention and control of invasive weeds in the mountainous regions of southern Zhejiang.

Abstract:By studying the effects of different nitrogen and phosphorus application ratios on the growth and physiological characteristics of Passiflora edulis Sims leaves,it can provide a reference for the scientific fertilization during the vegetative growth period of Passiflora edulis Sims.The pot experiment was carried out,and no fertilization(T1)was set as the control.The ratios of nitrogen to phosphorus were set as 5 treatments,and T2-T6 were 1∶1(T2),1.5∶1(T3),2∶1(T4),1∶1.5(T5),1∶2(T6),respectively.Then,the changes of fresh weight and physiological indexes of Passiflora edulis Sims leaves were analyzed.The results showed that:the fresh weight of leaves was significantly increased under T4 treatment at 100 days after fertilization treatment compared with T1,T2,T3 and T5;the content of superoxide anion in leaves under T4 treatment was significantly lower than that in T1,T3 and T5 treatments on the 25th,75th and 100th day;the APX activity in leaves under T4 treatment was significantly higher than that in the other five treatments;the MDA content of leaves under T4 treatment was significantly lower than that in T1 and T2 treatments,and the soluble sugar content of leaves was significantly higher than that of the control and other treatments;the proline content of leaves under T4 treatment was significantly higher than that in T1,T2,T5 and T6 treatments on the 75th and 100th day after the treatment.According to the comprehensive analysis,the application ratio(2∶1)of nitrogen and phosphorus in the vegetative growth period of Passiflora edulis Sims was the appropriate fertilization ratio to promote the growth of Passiflora edulis Sims leaves and improve the physiological characteristics.

Abstract:The study aimed to explore the effects of different water and nitrogen coupling on the economic yield,economic benefit,nitrogen use efficiency and water productivity of cabbage,in order to optimize the water and nitrogen management measures in the field of cabbage.By taking Cabbage Zhonggan No.15 as the research object,two irrigation modes[the conventional irrigation(W1),water-saving irrigation(W2,70%of conventional irrigation)]and four nitrogen application levels[0 kg·hm-2(N0),119.94 kg·hm-2(N1),239.88 kg·hm-2(N2),and 359.82 kg·hm-2(N3)]were set to analyze the effects of different water and nitrogen coupling on the economic yield,economic benefit,nitrogen use efficiency and water productivity of cabbage.The results showed that:The nitrogen application rate and irrigation mode had significant effects on the economic yield,nitrogen use efficiency and water productivity of cabbage(P＜0.05).The economic yield and water productivity increased first and then decreased with the increase of nitrogen application rate.Under the same nitrogen application rate,the nitrogen use efficiency was higher under the water-saving irrigation mode.he economic yield,economic benefit and water productivity of cabbage treated with W2N2 were the highest,which were 16.11 t·hm-2,16 544.73 yuan·hm-2 and 4.88%,respectively,and significantly increased by 14.34%,11.50%and 64.31%compared with W1N1 treatment,respectively.Therefore,the optimal water-nitrogen coupling mode for cabbage formation was as follows:330 t·hm-2 of irrigation amount and 239.88 kg·hm-2 of nitrogen application amount under the water-saving irrigation mode.The appropriate and moderate water-nitrogen coupling mode had the potential of increasing the production,high efficiency and water saving.

Abstract:In order to screen the early-maturing cauliflower(Brassica oleracea var.botrytis)hybrids with excellent and high yield,the botanical traits,commercial characteristics and yield performance of 32 early-maturing cauliflower combinations were comprehensively evaluated.The results showed that SHZ23-28,SHZ23-19 and SHZ23-27 exhibited outstanding yield performance,reaching 46 177.78 kg·hm-2,43 600.00 kg·hm-2 and 42 288.89 kg·hm-2,respectively,which were 35.3%,27.7%and 23.9%higher than that of the control cultivar Qingnong 65(CK).Among them,SHZ23-28 performed excellently in terms of high yield potential,marketability and adaptability.This hybrid cultivar could be harvested approximately 65 days after being planted in autumn.It had strong growth vigor and a relatively erect plant type,with dark green leaves covered in waxy bloom.The curds were high-round,fine-grained and milky white,featuring a semi-loose texture.The pedicels was light green,with the average weight of the single curd being around 1.4 kg.

Abstract:Styrax zhejiangensis is a protected plant species with an extremely small population endemic to Zhejiang Province.The study on the contents of carbon(C),nitrogen(N),phosphorus(P),potassium(K)and their stoichiometric ratios in the root zone soil of S.zhejiangensis and its associated plants is helpful to provide a scientific basis for the forest soil management in the conservation of this species.The soil samples were collected from the 0-20 cm soil layer around the roots of nine standard plant individuals,including S.zhejiangensis,and synergistic plants(Lithocarpus glaber,Camellia fraterna,Machilus thunbergii,Symplocos sumuntia),and competitive plants(Cinnamomum subavenium,Elaeocarpus decipiens,Ternstroemia gymnanthera,Eurya hebeclados).The contents of C,N,P and K were measured and the stoichiometric ratios were calculated.The results showed that:(1)The P contents in the rhizosphere soils of S.zhejiangensis and the synergistic plants were 1.28 g·kg-1 and 1.22 g·kg-1,respectively,which were significantly higher than those of the competitive plants(0.85 g·kg-1)(P＜0.05).However,there was no significant difference in soil C,N and K contents among the three types of plants.(2)The soil C/P,N/P,and K/P ratios in the rhizosphere soil of competitive plants were 32.23,1.41 and 10.56,respectively,which were significantly higher than those of S.zhejiangensis and synergistic plants(P＜0.05).There was no significant difference in soil C/N,C/K,or N/K ratios among the three types of plants.(3)The C content in soil was significantly positively correlated with the N content(P＜0.01),while C content and N content were significantly negatively correlated with P content,C/P and N/P ratios(P＜0.01).In conclusion,the synergistic plants may alleviate the phosphorus limitation for S.zhejiangensis during the growth by maintaining higher soil P content,while the competitive plants tended to compete for the limited phosphorus resources in the soil.For the conservation of this species,it was recommended to apply the phosphorus fertilizer appropriately,thin out the competitive plants moderately,and replant the synergistic plants to improve its habitat conditions.

Abstract:Protein disulfide-isomerase A3(PDIA3),a member of the PDI family,plays a core role in regulating the formation,cleavage,and rearrangement of protein disulfide bonds.This enzyme is closely associated with the occurrence and progression of various physiological and pathological processes,including thrombosis,cancer,neurodegenerative diseases,and viral infections,making it a highly promising target for drug intervention.In this study,the gene encoding PDIA3 was introduced into an Escherichia coli expression system,and recombinant PDIA3 protein with biological activity was successfully expressed and purified.Based on the establishment of a high-throughput screening method for PDIA3 inhibitors,110 small-molecule inhibitors of PDIA3 were identified from a natural product library comprising 4,080 compounds.Among these,Punicalagin,a major polyphenolic compound found in pomegranate peel,exhibited a relatively significant inhibitory effect,with a half-maximal inhibitory concentration(IC50)of 2.3 μmol·L-1.Molecular docking results suggested that Punicalagin may bind to the b'domain of PDIA3.This study provides candidate molecules and a theoretical basis for the development of PDIA3-targeted drugs.

Abstract:The effects of different seeding drums on the seeding quantity and uniformity of rice pot seedlings were explored to provide a basis for selecting the suitable drums for rice pot seedlings.Four types of sowing drums with different sowing hole diameters(a circular sowing drum with the sowing hole diameter of 9 mm and the depth of 4 mm,an elliptical sowing drum with the major axis of 12 mm,the minor axis of 9 mm and the depth of 4 mm,a small square sowing drum with the side length of 12 mm and the depth of 3 mm,and a large square sowing drum with the side length of 14 mm and the depth of 3 mm)were used to conduct the sowing experiments on 15 rice varieties.The grain shape structure of each rice variety and its sowing amount,leakage rate and uniformity under different sowing drums were analyzed.The results showed that the grain length,grain width and length-width ratio of the 15 rice varieties ranged from 7.97 to 12.23 mm,2.13 to 3.04 mm and 2.62 to 5.40,respectively.The leakage rate of rice sowing increased with the increase of grain length.Except for Yongyou 7850,the leakage rate of other rice varieties sowing with the circular sowing drums and small square sowing drums ranged from 6.55%to 85.12%,which was significantly higher than that of other sowing drums.The leakage rate of Yuzhenxiang in all four sowing drums was higher than 7.89%.The seeding rate and seeding uniformity decreased with the increase of grain length,when the grain length ranged from 7.97 mm to 9.82 mm,the seeding rates of different rice varieties using the elliptical seeding drums and large square seeding drums were 5.76-8.82 grains per hole and 4.03-5.25 grains per hole,respectively,and the seeding uniformity was 73.90%-87.31%and 61.40%-75.80%,respectively.When the grain length ranged from 9.97 mm to 10.93 mm,the sowing rates of rice using the elliptical seeding drums and large square seeding drums were 3.99-4.74 grains per hole and 2.05-3.25 grains per hole,respectively,and the sowing uniformity was 63.79%-75.60%and 28.78%-68.28%,respectively.By taking into account the hole leakage rate,sowing quantity and uniformity,when the length of rice seeds was 7.97-9.82 mm,it was suitable to use the large square sowing roller;when the length was 9.97-10.93 mm,it was suitable to use the elliptical sowing roller;when the single sowing quantity per hole of rice seeds was 3-5 grains,and the hole leakage rate was less than 5%and the uniformity was greater than 61.40%,which was suitable for rice pot seedling sowing;while for the longer Yuzhenxiang rice seeds,none of the four sowing rollers could meet the suitable sowing effect for pot seedlings,and it was not suitable for the sowing of rice pot seedlings.

Abstract:This study aimed to screen the compound substrate formula which was suitable for ginger plug seedling raising in Hunan area,and that took into account both the economic cost and seedling quality,in order to provide theoretical support and technical reference for the local ginger intensive seedling raising.In the experiment,the commercial seedling substrate was used as the control(CK),and peat,vermiculite and perlite were selected as the raw materials to prepare 11 kinds of composite substrates in different proportions for plug seedling test.After the end of raising seedling,the morphological characteristics,agronomic traits and related physiological indexes of the seedlings under different treatments were measured.The results showed that the physical and chemical properties of each substrate generally met the basic requirements of seedling raising.In order to comprehensively evaluate the growth status of ginger seedlings under the condition of plug seedling,the principal component analysis method was used to comprehensively analyze the indicators of seedlings and calculate the comprehensive score.Based on this,the comprehensive ranking of each index of ginger seedlings was as follows:T11＞T1＞CK＞T6＞T9＞T3＞T10＞T7＞T8＞T2＞T5＞T4.The further analysis showed that in the practice of ginger plug seedling,the best ratio of composite substrate was T11,that was,the volume ratio of peat,vermiculite and perlite(V peat∶V vermiculite∶V perlite)was 3∶2∶2.

Abstract:In order to develop a new type of functional biscuit,the low-gluten wheat flour,quinoa flour,and jujube flour were used as the primary raw materials,and the sensory evaluation score was used as the evaluation indicator.Then,the production process of quinoa and jujube biscuits was optimized through the single-factor experiments and response surface methodology.The results indicated that the highest sensory score of 91.43 was achieved under the following formulation:quinoa flour 27%,jujube flour 26%,white granulated sugar 21%,butter 30%,eggs 60%,skim milk 25%,and baking soda 0.5%.The biscuits produced under these conditions exhibited the golden color,uniform texture,and the well balanced aroma of quinoa and jujube,resulting in the most desirable flavor profile.

Abstract:The leaf color,as a persistent and stable visual element in the landscape design of garden plants,has increasingly demonstrated its esthetic and ecological value.The plants of the genus Rosa L.in the Rosaceae family exhibit rich species diversity and ornamental potential,yet the systematic research on their leaf color characteristics and the physiological mechanisms underlying coloration remains relatively scarce.By using 10 wild rose species as the materials,the L*a*b* color model was employed to quantify the leaf color parameters(L* brightness,a* red-green axis,b* yellow-blue axis),and the correlation analysis was performed combined with chlorophyll content,in order to analyze the physiological basis of leaf color variations in Rosa plants.The results showed that there were significant differences among different rose species in L*,a*,and b* values.Among them,Rosa beggeriana Schrenk and Rosa laxa Retz.exhibited the highest brightness,while Rosa xanthina Lindl.showed the lowest brightness.The a* value indicated a greenish bias across all species,though the greenness exhibited significant differentiation.The b* value revealed the distinct yellowish tendencies in some species.Through the quantitative phenotyping of rose varieties,a ranking and grading system was established based on the comprehensive score(S),with all varieties ranked from highest to lowest and categorized into three grades according to their rankings.The correlation analysis demonstrated a significant negative correlation between a* and chlorophyll content,confirming the physiological role of chlorophyll in red light absorption.L* values generally showed no significant correlation with chlorophyll,indicating that brightness was primarily influenced by the physical properties such as leaf surface structure.The relationship between b* and chlorophyll varied among species,reflecting the regulatory role of non-chlorophyll pigments.In this study,the leaf color of the rosa plants was objectively quantified,which provided the basis and reference for the evaluation and classification of rose germplasm resources and the color design of garden landscape.

Abstract:In order to explore the community structure characteristics and survival status of Ilex jiangmenensis,an endemic plant in Gudoushan Provincial Nature Reserve of Jiangmen,Guangdong Province,in November 2025,three 400-square-meter plots were set up to investigate the tree layer,shrub layer and herb layer in the concentrated distribution area of Ilex jiangmenensis in the Gudoushan Provincial Nature Reserve of Jiangmen,and the species composition,important value,diameter class structure and community overall connectivity were analyzed.The results showed that:(1)The species composition of Ilex jiangmenensis community was rich.There were 1 376 living standing trees recorded in the tree layer,belonging to 34 families,50 genera and 72 species.The dominant species was Itea chinensis.In the shrub layer,Rubus reflexus was the dominant specie,and the herb layer was dominated by Gahnia tristis Nees.(2)The diameter class structure of Ilex jiangmenensis showed an inverted"J"'type distribution,and the individuals with the diameter at breast height of 1-5 cm accounted for 71.80%,indicating that the community was well renewed.(3)The overall connectivity analysis showed that the tree layer,shrub layer and herb layer all exhibited the"overall insignificant correlation",and the relationship among the species was relatively independent.(4)A total of 8 strains of Ilex jiangmenensis were recorded in the investigation,with the diameters at breast height ranging from 0.6 to 1.3 cm and tree height from 0.4 to 1.6 meters.The results showed that Ilex jiangmenensis was not dominant in its community,with a small population size,but the community structure was stable.It was suggested that the long-term monitoring and protection of the species and its habitat should be strengthened to maintain its population survival.

Abstract:In order to solve the problems of the poor germination uniformity,low plug-seedling quality and poor seedling size uniformity of P.exaltatus,the effects of single priming agents,namely KCl(0.50%,0.75%,1%),KNO3(0.50%,0.75%,1%),PEG-6000(5%,10%,15%),and GA3(100,200,300 mg·L-1)on the seed germination were explored.Subsequently,the effects of different combinations of composite priming agent(PEG-6000+KNO3)were examined,and the effects of the screened composite priming agent on the seed germination and the seedling quality traits such as plant height and crown width of different P.exaltatus cultivars were explored.The results showed that among the single priming treatments,10%PEG-6000 or 0.75%KNO3 produced the best overall germination performance for the cultivar Joey.The composite priming treatment 10%PEG-6000+0.75%KNO3 was significantly better than that of single initiator and control treatment,achieving the highest germination rate(96.13%)and germination potential(88.90%),which were 9.15%and 28.04%higher than those of the control treatment.When this composite priming agent was applied to two cultivars,Matilda and Joey,it not only improved the seed germination rate and the vigor of germination,but also markedly enhanced the seedling quality.The optimized composite priming agent identified in this study could provide the technical support for improving the quality of seed germination and seedling cultivation of Ptilotus exaltatus.

Abstract:The main nutritional components of six Polygonatum species,including P.filipes,P.cyrtonema,P.kingianum var.grandifolium,P.kingianum Coll.et Hemsl,P.punctatum,and P.cirrhifolium in Qiandongnan Prefecture were systematically analyzed,which provided scientific basis for the evaluation,development and utilization of germplasm resources of Polygonatum in this area.The basic nutritional components such as moisture,protein,crude ash,vitamin C,reducing sugar and crude fat were determined by using the national standard method,and the composition and content of 16 amino acids were determined by using the amino acid analyzer.The results showed that there were significant differences in the nutritional components among the six species of Polygonatum germplasms in Qiandongnan Prefecture.Among them,the protein content of P.kingianum Coll.et Hemsl was the highest(14.91 g·hg-1),and the total amino acid content was 10.07 g·hg-1,with its essential amino acid pattern meeting the ideal protein standard.P.cyrtonema and P.kingianum var.grandifolium contained higher levels of reducing sugars(7.27 g·hg-1 and 6.84 g·hg-1,respectively).P.filipes exhibited superior performance in the contents of vitamin C(3.16 mg·hg-1)and crude ash(6.91 g·hg-1).All Polygonatum germplasms were rich in flavor amino acids(45.2%-49.8% of total amino acids),and showed balanced amino acid composition.Different Polygonatum germplasms in Qiandongnan Prefecture possessed distinct nutritional characteristics.The chemical diversity of these nutritional composition provided an important basis for the germplasm identification,quality evaluation and differential utilization of Polygonatum resources.

Abstract:Grouper nervous necrosis virus(GNNV)infects the nervous system of juvenile fish,with the mortality rates as high as 90%-100%,which was a bottleneck restricting the development of grouper aquaculture.The preparation of polyclonal antibodies against GNNV is essential for the in-depth study of viral protein functions and the development of related detection methods.In this study,an expression vector containing the protruding domain(P-domain)of the GNNV capsid protein,designated GNNV-P-pET-15b,was constructed and transformed into Escherichia coli BL21(DE3)for the prokaryotic expression.The purified GNNV-P protein was used as an antigen to immunize New Zealand white rabbits,generating the rabbit-derived polyclonal antiserum.The antiserum titer was determined by indirect ELISA,and its specificity was analyzed by Western blot.The results showed that:the 15 kDa soluble recombinant GNNV-P protein was successfully obtained by prokaryotic expression of GNNV-P-pET-15 b vector,and then further used as an antigen to immunize New Zealand white rabbits to prepare the rabbit-derived polyclonal antiserum against GNNV-P,with the titer of antiserum exceeding 1∶150 000 by using the method of indirect ELISA.The Westernblot showed that GNNV-P polyclonal antibody had high specificity.The results provided key biological materials for the subsequent study of GNNV infection mechanism and the development of related immunological detection methods.

Abstract:Exploring the exogenous factors that affected the bactericidal efficiency of antibiotics was a key entry point for analyzing the mechanism of bacterial resistance and tolerance,and had important theoretical and practical significance for optimizing the clinical anti-infection treatment.By taking Escherichia coli in the platform period as the research object,the effect of EDTA on the bactericidal activity of gentamicin was evaluated by exogenous addition of ethylenediaminetetraacetic acid(EDTA)combined with gentamicin.The concentration-gradient test and time-dependence test confirmed that:the inhibitory effect of EDTA on the bactericidal activity of gentamicin was significantly concentration-dependent,and this protective effect could persist throughout the whole drug treatment cycle.The results of mechanism exploration revealed that:by reducing the proton motive force(PMF)of Escherichia coli in the plateau phase,reducing the accumulation of intracellular reactive oxygen species(ROS),and alleviating the drug-induced cell membrane damage,EDTA finally weakened the bactericidal efficacy of gentamicin.The further research found that:the protective effect of EDTA was antibiotic class-specific,and only effective against aminoglycoside antibiotics(including gentamicin,tobramycin,streptomycin,kanamycin and amikacin),but had no significant effect on the bactericidal activity of quinolones and β-lactam antibiotics.The study could provide a reference for the clinical evaluation of the rationality of the application of EDTA in the combination of aminoglycoside antibiotics.

Abstract:In order to obtain the spatial distribution and dynamic changes of marine aquaculture quickly,accurately and in a large range,a remote sensing dynamic monitoring system for mariculture based on the deep learning was designed.By using C#language and under the Microsoft Visual Studio 2012 development platform,an integrated platform incorporating ArcGIS Engine,DevExpress components and a deep learning-based aquaculture zone extraction model was developed,which included the remote sensing data preprocessing,intelligent extraction of seawater aquaculture,spatial analysis and thematic mapping.In this paper,the demand design,functional module design,development environment and system implementation of the remote sensing dynamic monitoring system for mariculture were described in detail.By taking Zhao'an Bay in Fujian Province as an example,and based on the GF-2 remote sensing imagery,the MSU-ResUnet model was applied to rapidly and accurately extract the distribution of raft and cage aquaculture areas in Zhao'an Bay of Fujian Province in 2020,and the compliance analysis for planning was carried out.The system could quickly and accurately complete the extraction and dynamic analysis of large-scale marine aquaculture zones information,and improve the automation level and efficiency of aquaculture information extraction,which would provide scientific and intuitive decision support for the coastal aquaculture planning management and environmental protection.

Abstract:There were abundant forest resources in southern China.The cultivation of Chinese medicinal materials under the forest is an important way to solve the contradiction between the depletion of wild medicinal materials and the growth of market demand,thereby achieving the"symbiosis between forests and medicinal plants".At present,four kinds of characteristic medicinal materials planted under the forest in southern China,including Polygonatum cyrtonema,Paris polyphylla,Dendrobium officinale and Ganoderma lucidum,were all medicinal materials in great demand in the market.Although some progress has been made in the primary processing of these medicinal materials,there were still many problems,such as the backward primary processing technology,insufficient development of deeply processing products,and lack of uniform standards for the quality evaluation systems,which restricted the large-scale development of the processing industry.By focusing on the four kinds of characteristic medicinal materials under the forest in southern China,the research progress of their primary processing technology,deeply processing product technology,research and development,and industrial development trend was summarized,which provided theoretical support for the high-quality development of the medicinal materials industry under the forest in southern China.

Abstract:In order to evaluate the genetic diversity of Morinda officinalis germplasm resources in Fujian Province,a total of 27 germplasm resources collected from 10 sampling sites across 7 counties in Fujian Province were used as the research objects.Then,the phenotypic traits were systematically recorded and the pollen morphology was observed by using the electron microscopy.The genetic diversity was analyzed by ISSR molecular markers.The results showed that the pollen morphology of M.officinalis in Fujian Province was prolate(the P/E ratio of 1.66 to 1.99),with the polar axis length of 36.89 to 52.93 μm,and the equatorial axis length of 15.14 μm to 33.58 μm.The P/E ratio ranged from from 1.32 to 2.73,and exhibited the highest coefficient of variation(11.36％)among all the pollen traits.The leaf morphology(including elliptic,lanceolate,and ovate forms)and other phenotypic traits such as plant height,leaf number and branch number also displayed substantial variation,with the coefficients of variation ranging from 11.74％to 50.41％.The PCR amplification was performed by using ten ISSR polymorphic primers,generating 64 polymorphic bands with an average polymorphism percentage of 82.48％The results showed that 27 M.officinalis germplasms had high genetic diversity(Nei's gene diversity index H=0.305 2,Shannon's index I=0.457 8).The genetic distance analysis showed that the differentiation between populations was significant(Nei's genetic distance ranged from 0.080 0 to 0.693 1).The genetic relationship between M23 and M24 was the closest(similarity coefficient:0.923 1),and that between M26 and M2 was the farthest(similarity coefficient:0.500 0).The UPGMA clustering analysis divided the germplasm resources into three groups at the genetic distance of 0.654:Group Ⅰ(21 samples)representing the core genetic background,Group Ⅱ(5 samples)sharing common genetic characteristics,and Group Ⅲ(M8)as a unique ecotype.In summary,Morinda officinalis germplasm resources in Fujian exhibited rich genetic diversity,and clear geographical differentiation.It was recommended to give priority to the conservation of populations with high genetic diversity(such as M23/M24 population)and unique ecotypes(M8).The study could provide reference for the conservation,utilization and breeding of germplasm resources of M.officinalis in Fujian.

Abstract:In order to overcome the bottlenecks of traditional cultivation of Gastrodia elata Blume in Kang County,namely"deforestation and the one-year-on,three-year-off planting cycle",on the basis of more than 2 years of pilot test in Yangba Town,the key technologies such as the industrial production of bacterial strains,tridimensional layered substitute cultivation,intelligent environmental control,and fungus material circulation were integrated to form the technical regulations for the industrial tridimensional substitute cultivation of Gastrodia elata Blume.The regulation specified 28 technical parameters across seven major stages,including the producing area environment,fungus material preparation,tridimensional facilities,cultivation management,disease and pest prevention and control,harvesting,storage and transportation,and fungus residue reuse,so as to realize"three reductions and one increase"(40%water savings,60%land savings,100%oak wood savings,and over 30%yield increase),which provided a standardized model for Kang County to build the core area of Gastrodia elata Bl.factory in southeastern Gansu.