Improvement of pretreatment method of malachite green content by HPLC －fluorescence detection method in aquatic product
LI Xiuxia
LIU Xiaofang
WANG Zhen
SUN Xiejun
LUO Qiaoling
Abstract：To deserve better standard recovery ratio of malachite green , a sample pretreatment methods were optimized for high performance liquid chromatography coupled with fluorescence detector ( HPLC－FLD) detec－tion of malachite green residues from aquatic product.After optimal extraction solution of acetonitrile/dichlo－romethane (1:1, v /v), 0.024 mg/mL p－toluenesulfonic acid and 0.3 mg/mL hydroxylamine hydrochloride , 1 mL diethylene glycol was added to the solution to help liquid stratification , Potassium borohydride reduced , and PRS column purification , HPLC－FLD was used as detection , the average recovery ranged from 72.3% to 83.8%, and the relative standard deviation was ranged from 5.36% to 6.15%.This method was precise and suitable for determination of malachite green residues in aquatic products.
Keywords：Aquatic watermalachite greendetectionpretreatment method
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 212-216 )