Study on colloidal gold test strip for rapid detection of melamine residues in milk
TANG Yiwei
JIAO Xueqing
CUI Zhimeng
CHEN Jinai
REN Taotao
JIN Yuting
LI Ximing
LI Jianrong
Abstract：In order to develop a fast immunochromatography assay for melamine detection in milk, colloidal gold coupled with specific melamine antibody, the concentration of coating antigens, the amount of antibody la-beled colloidal gold and goat anti－mouse antibody were optimized in our study. The results show that the strip presented a best overcome when the concentration of coating antigen was 0.25 μg/mL, the amount of antibody labeled colloidal gold was 9.0 μL/strip, the dilution rate of goat anti－mouse antibody was 1:100. Under the optimal conditions, the limit of detection is 0.25 μg/mL, and the results of the test could be read by naked eyes within 5 min. Milk sample could be analyzed directly without any treatment. The developed methods in this study suit for onsite testing of melamine in milk.
Keywords：melaminecolloidaltest triprapid detection
Publication Date：2019-04-02
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 113-118 )