Optimization of Vibrio parahemolyticus enrichment medium by response surface methodology
LIU Xue-fei
LI Yu-jiao
LI Xiu-xia
LI Ying-chang
XIA Wen-shui
SUN Xiu-lan
LI Jian-rong
Abstract：Vibrio parahaemolyticus is a primary causative factor of our country's reported foodborne diseases affecting consumers in food safety and the development of aquaculture seriously.In a sense, the enrichment time determined the shelf life of seafood, which can effect on the quality and market value of seafood.To reduce en-richment time, the single factor tests and response surface methodology was used to optimize culture conditions and enrichment medium of Vibrio parahaemolyticus.Through the single factor analysis, the optimum growth tem-perature, oxygen concentration and culture mode of Vibrio parahaemolyticus ATCC 17802 were 37°C, breathable rubber plug and shake cultivation respectively.The Vibrio parahaemolyticus enrichment medium was optimized using the Box -Behnken Design of Design -Expert 8.0 software with mixed peptone, beef extract and NaCl as the main influence factors.The results showed that the quadratic regression equation had a high fitting degree. The optimal medium formulations as follows:casein peptone 6.75 g/L, tryptone 3.25 g/L, beef paste 4.76 g/L, NaCl 36.75 g/L.The optimized medium was used to verify the regression model.Vibrio parahaemolyticus cell density OD600 value (0.4632) was equivalent to 98.77%of the maximum predicted value (0.46895) of the model after incubation for 12 h.These results indicated that this regression model can be used for prediction and analysis of ATCC17802 growth dynamics.
Keywords：Vibrio parahaemolyticusresponse surface methodologyenrichment medium
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：9( 385-393 )