Determination of malachite green content by HPLC -fluorescence detection method in water
LI Xiu-xia
SUI Jun-fang
LIU Dan
LI Jiao
TIAN Xin
LI Jian-rong
Abstract：A gradient high performance liquid chromatography ( HPLC) coupled with fluorescence detection was developed for the detection of malachite green from water .Methylene chloride was used as extracting solvent assisted with ultrasonic method , the malachite green extract was purificated by MCX column after centrifuged , then eluted by 5%ammonia solution in methanol elution , Develosil C18 column was used as analytical column , 0.05 mol/L ammonium acetate (pH 4.5) -acetonitrile (20:80, v/v) as mobile phase, analyzed by fluores-cence detector , quantified by external standard method .The results showed that the linear range of malachite green was 0.05 μg/mL~1.0 μg/mL( r2 =0.9993), and the detection limit was 0.5 μg/L.When the added quality of malachite green in blank water was 1.0 μg, 2.5 μg, 5.0 μg, respectively, the average recovery ranged from72.0%to 86.4%, and the relative standard deviation was 5.4% ~7.5%.This method was precise and accurate , and suitable for determination of malachite green in aquaculture water .
Keywords：Malachite greenHPLCfluorescence detection method
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 279-283 )