微基因修饰雪旺氏细胞移植对大鼠脊髓损伤后GAP－43表达的影响
陈礼刚1
高立达1
毛伯镛2
杨立斌2
李开慧1
1.General Hospital of Chengdu Military Region,2.First Affiliated Hospital of West China Medical University
摘要：Objective To study the effect of microgene pSVPoMcat implanted to modify schwann cell on growth associated protein－43(GAP－43) expression after spinal cord injury in adult rats.Method Hemisected of the T8 segment of the spinal cord was performed for all the experiment rats.The rats were randomly divided into three groups as follows:Group A with microgene pSVPoMcat implanted to genetically modify SC;Group B with SC implanted ;Group C with hemisection of the spinal cord only.The changes of expression of GAP－43 in spinal cord were observed by immunochemistry with antibodies against GAP－43 .Simultaneous,the combined behavioral scores(CBS)was measured.Result There were not any different(P >0.05)among the three groups in first week and 12 week.There were significant diffeence(P<0.05)among three groups in 2nd,8th,and more dxpression of GAP－43 at the 2nd week in group A.The neurofunctional recovery was best in group A.Conclusion The microgene pSVPoMcat implanted to modify schwann cell can promote the expression of GAP－43 in spinal cord and functional recovery in adults rats after SCI.
机标关键词：雪旺氏细胞移植大鼠脊髓损伤基因修饰微基因
分类号：R68(骨科学（运动系疾病、矫形外科学）)
论文发表日期：2001-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：2( 142-143 )
英文信息