DOI: 10.12187/2025.05.012
Preparation of refined products of tobacco extracts based on membrane separation and column chromatography techniques
SHI Qingzhao
FAN Wu
REN Ruibing
CHAI Guobi
ZHANG Wenlong
ZHANG Qidong
ZHANG Jianxun
LI Helin
Abstract：To make full use of discarded tobacco leaves and develop a diverse range of refined products,tobacco extract was prepared from discarded tobacco leaves by heating extraction with an ethanol aqueous solution.The tobacco extract was then refined using membrane separation and two-dimensional column chromatography tech-niques.We used sensory evaluation to screen out characteristic flavor groups with prominent style features and explored the recombination process among these characteristic flavor groups.Py-GC-MS was used to analyze the recombinant products.The results showed that the yield of tobacco extract obtained by heating extraction with an ethanol aqueous solution was the highest,and the overall sensory effect was good.A total of 16 groups were obtained through refining,among which the sensory effects of the 5 nm retentate from membrane separation and the A1,C1,and E2 fractions from column chromatography separation showed distinct characteristics,serving as the characteristic flavor groups of tobacco extracts.Among the recombinant samples,samples 1#and 2#could enhance the characteristics of tobacco aroma,baking aroma,light aroma,and sweet aroma of cigarettes,and have a good overall sensory effect.The pyrolysis products of recombinant samples 1#and 2#were mainly composed of heterocy-clic and aromatic compounds,including sweet,light,nutty,and other aroma types.
Keywords：cigarettetobacco extractcharacteristic aroma component grouprecombinationsensory evaluation
Publication Date：2025-10-15
Online Publishing Date：2025-10-24(First online date of this platform, not the publication date of the document)
Pages：9( 101-109 )