DOI: 10.12187/2025.05.006
Investigation into the molecular mechanism of a Bacillus subtilis strain exhibiting high tolerance to crude glycerol
JIA Chenyang
ZHANG Fan
LIU Lanxi
WANG Guanglu
YANG Xuepeng
Abstract：The Bacillus subtilis strain with high tolerance to crude glycerol was selected as the research subject,and its tolerance mechanism to high-concentration crude glycerol was investigated using whole genome and transcriptome sequencing technologies.The results showed that 23 mutantgenes were identified in the evolved strain,involving key pathways such as the ABC transport system,nicotinic acid and nicotinamide metabolism,quorum sensing,and spore formation.Compared to the parent strain,the evolved strain exhibited significantly upregulated gene expression in pathways related to glycerol(ester)metabolism,the tricarboxylic acid cycle,purine metabolism,the ABC transport system,and energy metabolism.The synthesis metabolism of fatty acids and biotin of the evolved strainwere markedly enhanced.Furthermore,the evolved strain partially lost its spore-forming capacity,with spore formation rates decreasing by 38.0%and 52.3%during the stable middle and late stages,respectively.Additionally,six new components were detected in the cell membrane,along with a significant increase in both the types and concentration of fatty acids,thereby enhancing the stability of the phospholipid bilayer.This enhancement was the critical factor contributing to the strain's high tolerance to crude glycerol and its rapid growth.
Keywords：Bacillus subtiliscrude glycerol toleranceanalysis of molecular mechanismswhole genome sequencingRNA-sequencing
Publication Date：2025-10-15
Online Publishing Date：2025-10-24(First online date of this platform, not the publication date of the document)
Pages：11( 44-54 )