DOI: 10.12187/2025.04.011
Optimization of enzymatic hydrolysis process for expanded tobacco cut stem using combined crude enzymes of pectinase and laccase
QI Hanru
OUYANG Shaofeng
LI Yu
YANG Xuepeng
ZHAO Jianguo
Abstract：Pectinase-producing strain TS-1 and laccase-producing strain TS-2 were isolated from tobacco field soil and decayed tobacco stems.Morphological observation and molecular biological identification confirmed their taxo-nomic status as Aspergillus niger and Phanerodontia chrysosporium,respectively.Enzyme production conditions were optimized via single-factor experiments:for TS-1 pectinase production,the optimal parameters were tobacco stem powder concentration 50 g/L,fermentation temperature 30℃,and pH value 6.0;for TS-2 laccase produc-tion,the optimal conditions were tobacco stem powder concentration 50 g/L,(NH4)2SO4 concentration 0.6 g/L,and fermentation temperature 30℃.The hydrolysis conditions for pectin in expanded tobacco cut stems were opti-mized as crude pectinase dosage 240 U/g,hydrolysis time 4.0 h,liquid-to-solid ratio 1∶2,at 30℃;for lignin hydrolysis,the optimal conditions were crude laccase dosage 2.0 U/g,hydrolysis time 3.5 h,liquid-to-solid ratio 1∶2,at 30℃.The co-application of crude enzymes achieved optimal hydrolysis at pectinase dosage 170 U/g,laccase dosage 1.5 U/g,and hydrolysis time 3.0 h.Post-hydrolysis,the sensory evaluation score of expanded cut stems increased by 8.2 points compared to untreated samples,accompanied by a significant elevation in aroma con-stituent content.
Keywords：tobacco stempectinaselaccaseenzymatic hydrolysis processexpanded tobacco cut stemsensory qualityaroma constituent
Publication Date：2025-08-15
Online Publishing Date：2025-08-19(First online date of this platform, not the publication date of the document)
Pages：12( 96-107 )