DOI: 10.12187/2025.04.010
Metabolomic frofiling differences analysis of different grades of filler tobacco leaves after fermentation in Yunnan
LIU Hongjian
ZHOU Lequn
LI Guizhong
PENG Manjiang
WANG Xuefeng
ZHANG Guanghai
LI Zhihua
LIU Tao
Abstract：To elucidate the intrinsic mechanisms underlying the sensory quality differences in fermented filler tobacco leaves from Yunnan,we evaluated the sensory quality of six grades(B1,X1,C1,C2,C3,and C4)of post-fermentation filler tobacco leaves.Metabolomic profiling was performed using GC-MS,followed by comparative analysis via principal component analysis(PCA),orthogonal partial least squares discriminant analysis(OPLS-DA),and KEGG metabolic pathway enrichment analysis.The results revealed that,compared to other grades,C1 exhibited richer aroma,reduced irritation,and longer aftertaste.A total of 123 significantly different metabolites were identified between different parts of filler tobacco leaves,and 35 between different grades,primarily comprising heterocyclic compounds,aldehydes,ethers,carboxylic acids,and aromatic compounds.Notably,(E)-buta-2-enonitrile was a highly abundant and significantly different metabolite in all four comparison groups involving C1.The three metabolic pathways—cyanoamino acid metabolism,aromatic compound degradation,and butyric acid metabolism—were significantly enriched in the comparison groups involving C1.(E)-buta-2-enonitrile might contribute to metabolomic differences among different grades by modulating the accumulation and interaction of metabolites within these pathways,thereby leading to variations in sensory quality.
Keywords：filler tobacco leavesdifferent gradesensory qualitymetabolome(E)-buta-2-enonitrile
Publication Date：2025-08-15
Online Publishing Date：2025-08-19(First online date of this platform, not the publication date of the document)
Pages：10( 86-95 )