Study on the differences in antioxidant and lipid-lowering activities of different barley polyphenols

LEI Yuexin; CHENG Zhangchen; CHEN Jin; HE Yufeng; XIAO Xiang

doi:10.12187/2025.04.004

Abstract：Barley polyphenols were prepared by fermentation(using Lactiplantibacillus plantarum dy-1)and enzymatic hydrolysis(with sulfatase),respectively.The phenolic profiles of fermented barley polyphenols(FBP)and enzymatic hydrolysis barley polyphenols(EBP)were analyzed.The antioxidant capacities and lipid-lowering effects of FBP and EBP were further investigated.The results showed that the total phenolic contents of EBP and FBP were 78.35 μg GAE/mL and 146.56 μg GAE/mL,respectively.The absolute concentrations of vanillin,benzoic acid,2,4-dihydroxybenzoic acid,epicatechin,and salicylic acid differed significantly between the two barley polyphenol preparations.Under equivalent total phenol content conditions,vanillin,chlorogenic acid,and gallic acid were characteristic components of EBP,whereas benzoic acid,3,4-dimethoxybenzoic acid,and epicatechin were characteristic components of FBP.Compared to EBP,the DPPH and ABTS+scavenging capacities of FBP were enhanced by 36.8%and 13.3%,respectively.At a total phenolic content of 20 μg GAE/mL,both FBP and EBP effectively reduced intravital lipid droplet,accumulation and decreased triglyceride(TG,23.3%and 27.9%reduction,respectively)levels in Caenorhabditis elegans.Furthermore,both FBP and EBP enhanced locomotor activity in C.elegans,thereby accelerating lipid catabolism,and exerted lipid-lowering effects by suppressing the aberrant activation of the SBP-1 signaling pathway.Notably,EBP showed a more pronounced inhibitory effect on the insulin/insulin-like growth factor signaling pathway compared to FBP(P<0.05).In conclusion,under equivalent total phenolic content conditions,the compositional differences of barley polyphenols significantly modulated their antioxidant capacities and lipid-lowering activities.