DOI: 10.12187/2025.04.003
Effects of selenomethionine on the in vitro digestibility of myofibrillar proteins in pearl gentian grouper
XU Yucong
HE Shengqi
LUAN Hongwei
BU Ying
ZHU Wenhui
LI Jianrong
LI Xuepeng
Abstract：In this study,the effects of selenomethionine on the in vitro digestibility of myofibrillar protein(MP)in pearl gentian grouper were investigated using a static in vitro digestion model to simulate gastricointestinal digestion.The results showed that after simulated gastricointestinal digestion,the selenium content in the high-level selenomethionine group(Y2 group)was 0.130 mg/kg(gastric)and 0.072 mg/kg(intestinal),significantly higher than that in the control group(C group)and low-level selenomethionine group(Y1 group)(P<0.05).Specifically,the carbonyl content(2.02 nmol/mg and 2.59 nmol/mg),dityrosine content(3962 a.u.and 4062 a.u.),thiobarbituric acid(TBA)value(0.31 mg/kg and 0.32 mg/kg),and particle size(365 nm and 357 nm)in the Y2 group were significantly lower than those in the C and Y1 group(P<0.05).Additionally,the Y2 group exhibited higher UV absorption peak intensities and endogenous fluorescence intensities,indicating that high-level selenomethionine delayed MP oxidation and denaturation,thereby better maintaining the structural stability of MP during in vitro digestion.The in vitro digestibility(62.19%and 67.25%)and degree of hydrolysis(0.31 mmol/g and 0.34 mmol/g)in the Y1 group were significantly higher than those in the C and Y2 group(P<0.05).In conclusion,supplementation with selenomethionine not only enhanced the antioxidant capacity of MP but also improved their in vitro digestibility,suggesting that selenomethionine has potential applications in enhancing the nutritional quality and stability of fish proteins.
Keywords：pearl gentian grouperselenomethioninemyofibrillar proteinin vitro digestibilitysimulated gastricointestinal digestion
Publication Date：2025-08-15
Online Publishing Date：2025-08-19(First online date of this platform, not the publication date of the document)
Pages：10( 20-29 )