DOI: 10.12187/2025.02.008
The construction of biosensor with carbon quantum dots as fluorescence signal and its application in detecting Pb2+in honeysuckle
LI Haojia
HE Shihua
CAO Yize
GUO Xiyu
ZHU Youyu
ZHAO Weiqin
HUANG Chun
Abstract：Carbon quantum dots(G-CQDs)were prepared by hydrothermal method using grapefruit peel and fluorescence biosensor for Pb2+detection was constructed in honeysuckle.The morphology,structure and optical properties of G-CODs were characterized by circular dichroism spectroscopy,transmission electron microscopy,ultraviolet-visible absorption spectroscopy and fluorescence spectroscopy.The experimental conditions of the fluorescent biosensor were optimized and its detection performance was analyzed.The results showed that the G-CQDs were spherical and uniformly distributed.The average particle size was 2.41 nm,and the optimal excitation wavelength was 350 nm.The suitable experimental conditions for the fluorescence biosensor were as follows:Hemin concentration of 60 μmol/L,reaction time of 30 min,reaction temperature of 25℃,K+concentration of 10 mmol/L and pH value of 6.5.Compared with other metal ions(Ag+,Zn2+,Cd2+,Cu2+and Hg2+),the fluorescence biosensor had higher selectivity for Pb2+,and its fluorescence intensity increased with the increase of Pb2+mass concentration.In the linear range of 0.1～5.0 μg/mL,the linear relationship between the two was good,R2 was 0.998 0,and the detection limit was 0.063 9 μg/mL.Compared with the conventional method(ICP-MS method),the fluorescence biosensor had good accuracy,and had the advantages of green environmental protection,economical and effective,simple operation and so on.
Keywords：fluorescent biosensorcarbon quantum dotDNAzymeG-quadruplexhoneysucklePb2+
Publication Date：2025-04-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 72-79 )