Preparation of swim bladder peptide of Pseudosciaena crocea and its study on delaying skin aging

SUN Yanqing; DU Fen; ZHU Zongmin; FENG Xiaomei; SHANG Lili; CAO Wanxiu; LIU Chuyi

doi:10.12187/2025.01.013

Abstract：Using the superoxide anion radical scavenging rate as an indicator,single-factor experiments and response surface methodology were employed to optimize the preparation of peptides from swim bladder of Pseudosciaena crocea(HSBP).The HaCaT cell line,an immortalized human keratinocyte cell line,was employed to establish an oxidative damage model to evaluate the effects of HSBP on collagen and hyaluronic acid secretion,as well as the activities of antioxidant enzymes and β-galactosidase,to assess its oxidative damage repair activity.The 3D recombinant human epidermal barrier injury model was established to study the effects of HSBP on its tissue morphology and the expression of skin barrier related proteins,and to comprehensively evaluate its delaying aging effect on skin.The results showed that the optimal preparation conditions for HSBP were:enzymatic hydrolysis time of 4 hours,50 ℃,pH value of 7.5,and a bromelain addition of 3400 U/g.Under these conditions,the superoxide anion radical scavenging rate was 65.6％.The molecular weight of HSBP was 480 Da,with no irritation,sensitization,or cytotoxicity.HSBP could significantly promote the secretion of hyaluronic acid,type Ⅰand type Ⅳ collagen in oxidation-damaged cells,increase the activities of superoxide dismutase and glutathione peroxidase(GSH-Px),decrease the contents of malondialdehyde and β-galactosidase,and significantly increase the expressions of LOR,FLG,TGM1 and AQP3 in barrier damaged models,effectively repair the barrier damaged skin tissue,and significantly increase the number of viable cell layers.Therefore,HSBP could effectively protect skin cells from oxidative damage and repair skin barrier injury,thereby delaying skin aging.