DOI: 10.12187/2025.01.011
Preparation and performance evaluation of colloidal gold test strips for tobacco mosaic virus detection
WANG Chunqiong
LIU Kai
XIE Yonghui
ZHANG Ke
LIU Chunming
ZHANG Xiaowei
SUN Haowei
CHEN Dan
Abstract：To address the problems of the time-consuming and limited application of traditional tobacco mosaic virus(TMV)detection methods,colloidal gold test strips for rapid and qualitative detection of TMV were developed based on the double antibody sandwich method,and its preparation conditions were optimized.In addition,the limit,accuracy,and specificity of the test strip were detected.The results showed that the suitable preparation conditions for the test strip were buffer addition of 4 μL,antibody mass concentration of 10 μg/mL,and NC membrane of CN140 type and sample extraction solution of 0.02 mol/L PBS;The detection limit of this test strip for TMV-CP protein was 0.1 μg/mL,with no cross reactivity with tobacco bush top virus,tomato spot wilt virus,and potato Y virus,and good specificity;Applied to actual sample detection,the total coincidence rate with PCR was 95.0％,the positive coincidence rate was 93.3％,and the accuracy was good.This test strip could display the detection results within 10 min and was easy to operate.It could be used for rapid detection of TMVs in tobacco field.
Keywords：tobacco mosaic virusmonoclonal antibodycolloidal gold immunochromatographic techniquetest strips
Publication Date：2025-02-14
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 90-97 )