DOI: 10.12187/2024.04.011
Determination of petroleum ether extracts in flue-cured tobacco leaves by low-field nuclear magnetic resonance
FU Guangming
WEI Fengjie
CHANG Jianbo
JIA Baoshun
LI Zhixin
LAI Miao
YANG Xiaopeng
JI Xiaoming
Abstract：In response to the problems of slow detection speed,cumbersome operation,and solvent pollution in the detection of petroleum ether extract in tobacco leaves using the current traditional Soxhlet extraction method,low field nuclear magnetic resonance technology was used to detect the petroleum ether extract content in tobacco leaves.The detection parameters such as drying time,sample quality,echo time,and number of repeated scans were optimized to verify the repeatability and accuracy of the method,and the detection results were compared with the traditional Soxhlet extraction method.The results showed that 3.0 g of tobacco leaf powder passing through a 40 mesh sieve was dried at 80℃for 4 hours.Under the conditions of echo time of 0.25 ms and 8 repeated scans,the petroleum ether extract detection results were good.The standard curve R2 reached 0.999 81,and the relative standard deviation of repeatability was 1.45%to 2.99%.The intra day and inter day precision were 2.61%and 1.10%,respectively.The correlation coefficient between the detection results of this method and the Soxhlet extraction method reaches 0.998 55,with an absolute error within 0.02%～0.35%and a relative error within 0.26%～3.69%.Compared with the traditional Soxhlet extraction method,this method is simple,fast,accurate,and solvent free,making it suitable for the accurate quantification of petroleum ether extracts from large quantities of flue-cered tobacco leaves.
Keywords：low-field nuclear magnetic resonancepetroleum ether extracttobacco leaf powderrepeatabilitypreci-sion
Publication Date：2024-08-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：9( 80-88 )