DOI: 10.12187/2024.02.005
Antibacterial activities and mechanisms of the major compositions of Allium tenuissimum flower essential oil against Listeria monocytogenes
WU Chengyu
LI Yingqiu
Abstract：This study investigated the antibacterial activities and mechanisms of the major compositions(allyl methyl disulfide and dimethyl trisulfide)of Allium tenuissimum flower essential oil against Listeria monocytogenes.The antibacterial activities and mechanisms of these two compositions against L.monocytogenes were evaluated by minimum inhibitory concentration(MIC),minimum bactericidal concentration(MBC),cellular morphologies,etc.These results showed that MICs of these two compositions were both 1.0 mg/mL,and MBCs were both 2.0 mg/mL.The 2.0 mg/mL of allyl methyl disulfide and dimethyl trisulfide rigorously impaired cellular morpholo-gies and cytomembrane structures of L.monocytogenes.The leakage of β-galactosidases and proteins significantly increased when bacterial cells were treated with these two compositions at 4.0 mg/mL.ATPase activities(0.52 U/mg prot and 0.55 U/mg prot)of the cells treated with allyl methyl disulfide(4.0 mg/mL)and dimethyl trisulfide(4.0 mg/mL)were distinctly lower than the activity of control(3.05 U/mg prot,P<0.05),respectively.Fur-thermore,the contents of DNA for bacterial cells were remarkably decreased after being treated with these two com-positions.Thus,allyl methyl disulfide and dimethyl trisulfide inhibited activities of L.monocytogenes via destruction of cytomembrane structures,inactivation of ATPase and reduction of DNA contents in bacterial cells.
Keywords：Allium tenuissimum floweressential oilallyl methyl disulfidedimethyl trisulfideListeria monocyto-genesantibacterial activity
Publication Date：2024-04-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 36-42 )