Study on the preparation of taurine from cooking liquid of Ruditapes philippinarum by enzymatic hydrolysis
HU Kailun
GAO Rongchun
YANG Fengsheng
LIU Wenzhuan
YANG Jingfeng
Abstract:This study aim to explored the preparation and purification technologies of taurine from the cooking liquid of Ruditapes philippinarum using enzymatic hydrolysis.The results showed that the alkaline protease was the optimum enzyme for extracting taurine.Under the conditions of enzymolysis temperature 45 ℃,enzymolysis time 3.5 h and enzymolysis pH =8.0,the purity and recovery of taurine reached 8.67% and 95.80%,respectively.After removing salt using electrodialysis,the purity and recovery of taurine reached 21.30% and 89.59%,respectively.After elution on cation exchange resin,the purity and recovery of taurine reached 31.18% and 83.64%,respectively.After the ethanol recrystallization,the purity of taurine reached 97.28%.
Keywords:Ruditapes philippinarumcooking liquidtaurineenzymatic hydrolysiselectrodialysis
Publication Date:2018-01-01
Online Publishing Date:2025-08-15(First online date of this platform, not the publication date of the document)
Pages:8( 26-33 )