Determination of the content of neutral aromatic components from cigarette mainstream smoke by the separation of semi-preparative HPLC and GC-MS
JIA Chun-xiao
ZHANG Yue-li
CHEN Zhi-fei
CAI Li-li
SUN Zhi-tao
MA Yu-ping
MAO Duo-bin
Abstract：A method for determination of the content of neutral aromatic components from cigarette mainstream smoke by semi-preparative high performance liquid chromatography (semi-preparative HPLC) coupled with gas chromatography-mass spectrometry (GC-MS) was developed:the fraction collection time was determined to three time periods by optimizing the conditions of semi-preparative HPLC in order to ensure that the compounds to be measured don’t cross in the fractions.The selected ion chromatogram showed that all target compounds could be separated completely in three fractions and the method could satisfy the demands of quantitative analysis.Quantitative analysis was performed by establishing a GC-MS internal standard curve.The analysis results showed that the correlation coefficients were greater than 0.999 0,the limits of detection (LOD) were 0.55 ～ 13.06 μg/kg and the limits of quantitation (LOQ) were 1.85 ～ 43.55 μg/kg.It showed that the method has high sensitivity.The results of sample determination and recovery experiment showed that the average added standard recovery of the neutral aromatic components from cigarette mainstream smoke was in the range of 64.39％ ～93.57％ and the relative standard deviations were less than 10％.It showed that the method could satisfy the requirement of quantitative analysis of trace compounds,with good reproducibility.
Keywords：semi-preparative HPLC (high performance liquid chromatography) separationGC-MScigarette mainstream smokeneutral aromatic components
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：10( 63-72 )