Effects of different enzymolysis conditions on the protein hydrolysis degree and antioxidant activity of tobacco leaf
XU Chun-ping
JIANG Yu
ZENG Ying
MA Kuo-yan
TAN Lan-lan
SHENTU Hong-qian
QU Li-li
MENG Dan-dan
DAI Ya
Abstract：Tobacco leaf protein was extracted by alkali-solution and acid-isolation method from upper leaves in the field.The tobacco protein was hydrolyzed using trypsin,papain or protamex,respectively.The effect of different enzymolysis conditions on leaf protein hydrolysis degree and the DPPH clearance rate of tobacco leaf protein polypeptide were investigated.The results showed that the optimal enzymolysis conditions of protein peptide were different with those of the highest DPPH clearance rate of protein polypeptide.Using papain as an example,the optimal hydrolysis conditions for tobacco protein hydrolysis degree were enzymolysis temperature 50 ℃,hydrolysis time 0.5 h,enzyme amount 3％,pH =8.The optimal hydrolysis conditions for DPPH clearance rate were temperature 40 ℃,enzvmolvsis time 2 h,enzyme amount 3％,pH =7.This indicated that the degree of hydrolysis of protein polypeptide was not positively correlated with its antioxidant activity.Protein peptide can be used as substrate for Maillard reaction or antioxidant,and it may be chosen corresponding optimal enzymatic conditions for different purposes.
Keywords：discarded tobacco leaftobacco leaf protein polypeptideenzymatic hydrolysisantioxidant activityhydrolysis degreeclearance
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 56-62 )