Analysis of prokaryotic mirobial community diversity with the capability of caproic acid-producing and lactic acid-utilizing in the pit muds of Luzhou-flavor liquor
ZENG Tian
HU Xiao-long
MA Zhao
CUI Yuan-yuan
WANG Jing-jing
HE Pei-xin
LI Cong-cong
NIU Guang-jie
LI Hong
Abstract:The caproic acid-producing and lactic acid-utilizing consortiums were constructed based on microbe in pit muds of Luzhou-flavor liquor from one wine corporation in He'nan.The prokaryotic microbial community was analyzed using high-throughput sequencing based on the V4 variable regions of 16S rRNA gene.The results showed that the species diversities in both constructed consortiums were lower than those in pit muds,which was accompanied by the obvious changes in composition of dominant microbe.The dominant prokaryotes in the caproic acid-producing consortiums were the Firmicute Clostridium and Ruminococcus.The dominant prokaryotes in the lactic acid-utilizing consortiums belomged to the Firmicute Bacillus,Clostridium,Sporanaerobacter,Soehngenia,Oscillospira,Ruminococcus,Paenibacillus,Caldicoprobacter,Sedimentibacter,Dorea,Coprococcus and Tepidimicrobium.This study will be conducive to clarify the taxonomic status of caproic acidproducing and lactic acid-utilizing consortiums in pit muds,and provide theoretical support for realizing caproic acid-producing and lactic acid-utilizing in production of Luzhou-flavor liquor through microbial directional bioaugmentation.
Keywords:microbe in pit mudshigh-throughput sequencingLuzhou-flavor liquorcommunity diversity
Publication Date:2017-01-01
Online Publishing Date:2025-08-15(First online date of this platform, not the publication date of the document)
Pages:8( 12-19 )