Component analysis of volatile metabolites of culturable Clostridium spp. Isolated from pit muds of Luzhou-flavor liquor based on HS-SPME-GC-MS
HE Pei-xin
LI Cong-cong
HU Xiao-long
CHI Lei
MA Zhao
GUO Yan-feng
Abstract：A total of 88 pure cultures was ohtained from the pit muds of one wine corporation following separation and purification culture.The 75 strains of bacteria were assigned into 8 phylogenetic clusters (cluster Ⅰ-Ⅷ) based on the analysis of 16S rRNA gene sequences and phylogenetic tree.In them,the most similar bacteria were C.tyrobutyricum,C.butvricum,C.indolis,C.acetobutylicum,C.bifermentans,C.sporo genes,C.cadaveris and C.kluyveri,respectively.The volatile metabolites of 8 representative Clostridium spp.were analyzed using headspace solid-phase microextraction (HS-SPME) method followed bv gas chromatography-mass spectrometry (GC-MS).A total of 75 volatile metabolites was detected,including 23 alcohols,20 acids,14 esters,2 aldehydes,2 ketones and other 14 volatile substances.The principal component analysis revealed that the representative strain RL1 (C.tyrobutyricum) and EL7 (C.butyricum) were mainly related to the generation of acetic acid and butyric acid,and EL46 (C.kluyveri),RL20 (C.acetobutylicum) and RL10 (C.indolis) mainly contributed to the formation of caproic acid and ethyl hexanoate,and EL12 (C.bifermentans) and RL23 (C.sporogenes) were mainly associated with the S-methyl acetate,l-Decyl alcohol and octanol,and EL43 (C.cadaveris) was mainly correlated to the biosynthesis of various esters.
Keywords：Luzhou-flavor liquorpit mudsClostridium spp.HS-SPME-GC-MSvolatile metabolite
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：11( 1-11 )