Process optimization of complex enzyme modification of millet bran dietary fiber
JIANG Long-bo
LYU Jing
ZHANG Xi-wen
LI Ping
DU Wen-juan
ZHANG Gui-ying
SHEN Rui-ling
Abstract：The dietary fiber (DF) was obtained from Shanxi millet bran by thermostable α-amylase,glucoamylase and neutral protease.Then the SDF was prepared from DF by the method of complex enzyme (cellulosexylanase) modification.The enzyme modification process was further optimized by the response surface design based on the single factor experiment.The optimum process conditions were as follows:total amount of complex enzyme1.73％,ratio of cellulose and xylanase 1:1.74,hydrolysis temperature 50 ℃,hydrolysis time 1.44 h.The SDF and IDF yields were 12.46％ and 85.12％ under this condition,respectively.The optimization design accurately predicted the yield of SDF obtained from millet bran DF.Importantly,the process operation was simple and required no special equipment,which possessed certain theoretical and practical significance for the comprehensive utilization of millet bran and the industrialization of SDF.
Keywords：millet brandietary fibercomplex enzyme modification
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 16-23 )