Study on quantitative detection of tPSA and fPSA by fluorescent chromatography
QIAN Cun
WANG Yun-long
MING Liang
LI Yu-lin
WANG Ji-chuang
CHENG Lei
CHENG Chun-jie
GAO Yu-hong
YAN Sheng-hui
Abstract：Conjugating the EDC activated fluorescent microspheres with antibody to prepare the fluorescence chromatographic strip for simultaneous detection of tPSA and fPSA.The performance analysis and methodological comparison for the test strip were further performed.The results showed that the sensitivity for PSA and fPSA detection by the test strip were 0.08 ng/mL and 0.06 ng/mL, respectively.The coefficient of variation within and between groups was less than 10% and 15%, respectively.The recovery rates ranged from 95% to 105%.The specificity of the test strip was well.The results revealed a good correlation between self-prepared test strip and electrochemiluminescence detection, which could satisfy the needs of aided diagnosis of small-scale hospitals.
Keywords：tPSAfPSAfluorescent microspheresfluorescent chromatography
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 37-42 )