Condition of variable temperature fermentation of poly-γ-glutamic acid production by B.amyloliquefaciens YP-2
ZHAO Xiao-hang
SHEN Ya-peng
LIU Pei-yang
JI Xiao-yue
ZHANG Shi-min
XU Shu-xia
WU Kun
Abstract：The effects of constant temperature fermentation time on poly-γ-glutamic acid (γ-PGA) production by B.amyloliquefaciens YP-2 was investigated under different temperatures (31 ℃, 33 ℃, 35 ℃, 37 ℃ and 39 ℃).The scheme of step-wise temperature control (0~4 h, 35 ℃;4~7 h, 31 ℃;7~10 h, 33 ℃;10~26 h, 37 ℃;26~60 h,31 ℃) was formulated following the kinetic analysis of γ-PGA fermentation.Under the variable temperature fermentation condition, the maximum cell productivity and γ-PGA yield reached 1.66 g/(L·h)-1 and 29.50 g/L, respectively, which was 4.4% and 11.8% higher than those in optimal constant temperature at 37 ℃.The results indicated the variable temperature fermentation effectively improved the γ-PGA production by B.amyloliquefaciens YP-2 comparing with constant temperature fermentation.
Keywords：varioble temperature fermentationB.amyloliquefacienspoly-γ-glutamic acid(γ-PGA)kinetic analysis
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 30-36 )