Tag identification of flue-cured tobacco varieties by multiplex PCR technique
JIA Meng-zhu
SUN Jiu-zhe
SU Dong-ying
YANG Jin-chu
WANG Er-bin
XU Heng
WANG Yong-chao
WANG Jun-ting
MENG Dan-dan
MA Lin
Abstract：With 12 varieties of flue-cured tobacco as a template,the conditions for primer concentration and annealing temperature for 6 pairs of SCAR primers were optimized.Multiplex PCR fingerprints and identification routes of 12 flue-cured tobacco varieties were established by multiplex PCR.The results showed that multiplex PCR had the advantages of high efficiency, high speed, time saving and low cost compared with ordinary PCR.Six pairs of SCAR primers had good stability and specific band specificity, which could distinguish 12 flue-cured tobacco varieties accurately.
Keywords：multiplex PCR techniqueSCAR tagidentifica-tion of flue-cured tobacco varietiesfingerprints map
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 51-57 )