Research progress of aflatoxin B1 detection based on nucleic acid aptasensor
JIANG Liying
LIU Shuai
ZHANG Pei
REN Linjiao
YAN Yanxia
ZHENG Xiaowan
CHEN Qinghua
JIANG Suxia
Abstract：There is a high toxicity and carcinogenicity of aflatoxin B1（AFB1）in food. Current detection of aflatoxin B1 is performed by a variety of methods including thin layer chromatography, high performance liquid chromatography, liquid chromatography-tandem mass spectrometry and immunoassay, but they all have some limitations. AFB1 detection research had obtained certain achievements based on nucleic acid aptasensor.The three new detection technology based on the electrochemical nucleic acid aptasensor,optical nucleic acid aptasensor and PCR nucleic acid aptasensor had advantages and disadvantages.A new idea in which glucose meter was used as a detection instrument and combined with nucleic acid aptasensor for AFB1 qualitative analysis were to be experimental veried.Combining nucleic acid aptasensor with magnetic nanoparticle united with bio-recognition elements as the adsorbent for solid phase extraction (SPE) was expected to be a breakthrough in the future.
Keywords：aflatoxin B1nucleic acid aptasensorglucose metersample pretreatment
Publication Date：2017-03-02
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 40-47 )