Study on preparation and antioxidant activity of the Lycium barbarum polypeptide
LI Song-lin
LIN Jing
JIANG Chang-xing
CHEN Xiao-ming
WANG Xiao-hua
YE Hua
WANG Hui
Abstract：Enzymatic preparation conditions of Lycium barbarum bioactive peptides which were derived from hydrolyzation by compound of neutral protease and papain were studied.And then enzymatic hydrolysate was purified by ion-exchange column chromatography and gel filtration chromatography in order to prepare antioxi-dative bioactive peptides.Based on the DPPH· radical scavenging rate,the preparation method was opti-mized by response surface methodology.The optimal temperature,composite enzyme quality ratio,time and pH were 51 ℃,12.65,4.3 h and 7.0,which resulted in a DPPH· radical scavenging rate of (23.32 ± 0.47)%.Then the enzymatic hydrolysate flowed through anion-exchange chromatography and sephdex G-25 gel chromatography in order to get purified polypeptide.Component A-1 was acquired which had the DPPH· and OH· removal rate of (72.65 ±1 .84)%and (58.72 ±1 .88)%respectively.DPPH·,ABTS+· and OH· radical scavenging activity of protein peptide A-1 increased with the increase of concentration.
Keywords：Lycium barbarum polypeptideenzymo-lysispurificationantioxidant activity
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 31-37 )