Simultaneous determination of 20 restricted food additives in fruit and vegetable juice beverage by HPLC
LIU Sheng-nan
ZHANG Guo-qing
WANG Su-fang
GUO Bao-bao
QUAN Ming-zhu
SHI Zhi-ge
Abstract：A high performance liquid chromatography (HPLC)method was developed for simultaneous deter-mination of 20 restricted food additives in fruit and vegetable juice beverage.The method was based on extrac-tion with methanol mixed with water.The separation was achieved on a ZORBAX SB-C1 8 column by using methanol and NH4Ac as mobile phase with the gradient eluent.Components were detected with a diode array detector (DAD).The quantification was performed by the external standard method.The results showed that the method exhibited an excellent linearity in the concentration range of 0.5 ~50.0 μg/mL with a correlation coefficient greater than 0.999 0 and a good repeatability with relative standard deviation of 1 .5% ~4.9%.The limits of detection were between 0.5 mg/kg and 1 2.5 mg/kg,the limit of quantitation were between 2.5 mg/kg and 40.0 mg/kg,and average recoveries ranged from 80.1 % to 1 00.9%.The test result of the additives content of ten types of fruit and vegetable juice beverage on the market showed that the present method was simple,and had good separation effect.Its recovery and accuracy could meet the requirements for the simultaneous determination additives in fruit and vegetable juice beverage.
Keywords：high performance liquid chromatography(HPLC)food additivesfruit and vegetable juice bever-age
Publication Date：2016-07-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 23-29 )