Preparation of monolithic materials and applications in proteomic chromatographic separation
WU Ci
LIANG Yu
LIANG Zhen
ZHANG Li-hua
ZHANG Yu-kui
Abstract：With the advantages of facile preparation,fast mass transfer,low backpressure and easy modifica-tion,monolithic materials have been widely used in chromatographic separation.In order to familiarize profes-sionals of home and abroad with the present situation of monolithic materials,this review mainly summarizes the preparation methods of different kinds of monolithic materials (including organic polymer monoliths,silica-based monoliths,organic-inorganic hybrid silica monoliths)and their applications in the separation of proteins or peptides in proteomics.It was pointed out that compared with packed column,monolithic packed column efficiency was to be further improved from the performance of monolithic materials,such as specific surface area,pore size distribution,stablity and hydrophilicity.Taking advantage of the low backpressure of monolithic materials,the capillary monolithic column with superlong and superfine inner diameter was prepared in order to make high efficient separation analysis of macro proteomics samples.With the further study of monolithic materials preparation technology,monolithic materials will play more important role in the fields of food safety, life science and environment.
Keywords：monolithic materialspreparation methodchromatographic separationproteomics
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：14( 1-14 )