Determination of antioxidant activity of Visum ardiculatum in vitro
ZHANG Yu
ZHANG Xiao-na
XUE Qiao-li
XIAO Rong
LIN Qi
HU Yong-jin
Abstract：Visum ardiculatum was extracted by water bath and ultrasonic,and its four fractions of chloroform, ethyl acetate,n-butanol,and water were prepared.The free-amino acid,total polyphenol,tea polysaccharides and flavonoids contents Visum ardiculatum were determined.So were the scavenging effects of different parts of Visum ardiculatum on DPPH radical,ABTS radical,superoxide anion and hydroxyl radical and FRAP value. The results showed that the antioxidant activity of Visum ardiculatum in vitro was potent.The scavenging effects of all the fractions on ABTS radical reached 98% and that of DPPH radical reached 88%.The synthet-ical value calculated by fuzzy assessment was respective of the 5 antioxidant assays.The highest was 4.66 of water extracts by water bath,the lowest was 0.21 of chloroform fraction.It was largely due to the total poly-phenol(P <0.05 ) and flavonoids contents (P <0.05 ).The content of tea polysaccharides reached 5 468.08 mg/1 00 g.
Keywords：Visum ardiculatumantioxidantcorrelationfuzzy assessment
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：10( 39-48 )