Fabrication of sensitive layers of the 3D graphene electrochemical biosensor for heavy metal ions detection
REN Su-yun
JI Hong-fei
ZHANG Zhi-hong
WANG Ming-hua
HE Ling-hao
Abstract：The 3D graphene was prepared by redox mothod and then was functionalized by amino-modified (G-NH2 ).The single-stranded DNA could immobilize onto the surface of the fabricated biosensor via the elec-tro-static interaction between DNA and G-NH2 and coordination with the relative heavy metal ions (Ag +, Hg2 +,Cu2 +).The surface morphology was determined using transmission electron microscopy (TEM)and scanning electron microscopy (SEM).Atom force microscopy (AFM)and X-ray photoelectrons spectroscopy (XPS)were applied to measure the chemical structure and componenets of the fabricated G-NH2 before and after being immobilized DNA.The results showed that the surface of 3D G-NH2 was smooth.Signal changes of XPS peak demonstrated that DNA were immobilize onto the surface of 3D G-NH2 .The electrochemical imped-ance spectrum was used to characterize the electrochemical perforamance of the biosensor during the procedure of the detection of heavy metal ions.It explored that the Rct values were changed significantly after DNA was immoloilized onto the surface of 3D G-NH2 and then coordinated with the relative heavy metal ions.This study demonstrated this kind of biosensor could be used to adsorb biomolecules and detect the co-exist system of many heavy metal ions.
Keywords：3D grapheneself-as-sembling layerDNAheavy metal ion detec-tionelectrochemical biosensor
Publication Date：2016-05-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 14-20 )