Process discussion on preparation of bioactive peptide in Chinese medicine residues by protease hydrolysis
LU Fu-ping
YANG Ji-han
WANG Yong-shuai
QING Kuai
WANG Hong-bin
Abstract：Using the residues of Chinese meicine Xuebijing as materials,bioactive peptide was prepared by protease hydrolysis.The hydrolysis residues effect of acid protease,neutral protease and alkaline protease was investigated.On this basis,the appropriate proportion of compound protease hydrolysis residues was studied, and the suitable hydrolysis time of compound protease was confirmed by comparing the index of different hy-drolysis time,such as protein extraction rate,hydrolysis degree and the antioxidant index DPPH free radical clearance rate.The results showed that the neutral protease compounded with alkaline protease was selected to prepare bioactive peptides,appropriate ratio was 31;the suitable hydrolysis time of complex protease was 4 h,under the condition,the protein extraction rate was 81%,and the antioxidant index DPPH free radical clearance rate was 39.8%.
Keywords：Chinese medicine residuebio-active peptideprote-asehydrolysis
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 12-16 )