Optimization of multiple PCR reaction system in flue-cured tobacco leaf
MA Lin
GUO Miao-miao
SU Dong-ying
SUN Jiu-zhe
WANG Er-bin
LIU Shan-yu
TIE Jin-xin
JIA Meng-zhu
Abstract：Using the single factor and orthogonal experiments with the flue-cured tobacco varieties of zhongyan100 as raw material and two SCAR primers to study the multiple PCR reaction system in the inter-action between the main ingredients of flue-cured tobacco leaves,the best suitable multiple PCR reaction system was established for roast tobacco including DNA template 45 ng,Mg2 + concentration 2. 50 mmol/L,dNTP concentration 0. 40 mmol/ L and Taq enzyme dosage 4. 00 U on the 20 μL system. The stripe of the system is clear and it can shorten the identification time,laying a foundation for using multiple PCR technology to identify flue-cured tobacco varieties.
Keywords：multiple PCR reaction systemflue-cured tobacco leafSCAR
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 30-33 )