Study on biosynthesis of aloe-emodin
ZHONG Gui-fang
FAN Pan
CUI Gai-beng
Abstract:Recombinant glucosidase was used to catalysis biosynthesize of aloe-emodin through aloin. Single factor experiment and uniform design were used to optimize the biosynthesize conditions. As the result,the optimal conditons of aleo-emodin’s biosynthesis were as following:reaction time 4 h,combinant glucosidase concentration 50 U/ mL,the ratio of tert-butanol and water 5: 5(v/ v),reaction temperature 43 ℃ ,and pH value 5. 2. Under those conditions,the highest yield was 92. 18% .
Keywords:biosynthesizealoinaloe-emodinrecombinant glucosidase
Publication Date:2015-04-02
Online Publishing Date:2025-08-15(First online date of this platform, not the publication date of the document)
Pages:5( 26-29,41 )