Separation and purification of laccase used for tobacco stem lignin degradation
ZHANG Rui
LI Yuan-shi
KOU Xiao-teng
ZHU Liang-hua
YU Xiang
MA Lin
Abstract：After the laccase crude enzyme which was obtained by fermenting Coriolus versicolor had been concentrated and hyperfiltrated,grading salting precipitation and DEAE-Sepharose-F.F.column chromatog-raphy had been taken to separate and purify the laccase fermented liquid with ammonium sulfate as the sal-ting-out agent.SDS-PAGE electrophoresis results showed that pure laccase could be obtained,the molecular weight was about 64.4 kD,enzyme was purified for 22.80 times by the purification process,the enzyme ac-tivity recovery rate was 36.32%.These proved that the laccase which was produced by Coriolus versicolor could be well purified.Using the enzyme solution in the experiment stem lignin degradation,the results showed that the intrinsic quality of the processed tobacco stems had been improved to some extent,miscel-laneous gases and irritating significantly reduced,wood gas also significantly reduced,aroma and aftertaste slightly improved.
Keywords：tobacco stem ligninlaccaseseparation and purificationCoriolus versicolor
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 5-8,12 )