Determination of 3,4,5-trihydroxy benzoic acid in tobacco essence and flavor
DENGMei-zhong
CHENDu-jian
HUANGSheng-han
ZHOUYue-fei
Abstract：A method for the detection of 3,4,5-trihydroxy benzoic acid in tobacco essence and flavor was developed by using high performance liquid chromatography.After oscillate-extraction by ethanol,the sam-ples were separated by a C18 column (100 mm ×3.9 mm,5 μm),using methanol and 0.05% phosphoric acid (694)as mobile phases by isocratic elution,and detected by diode array detector with the wave-length of 272 nm.The experimental results showed that:within the range of 0.1 ~10.0 mg/L,the linear correlation coefficient for 3,4,5-trihydroxy benzoic acid was 0.999 4,the low detection limit was 1.01 μg/g,the recovery was 93.1%~103.2%,the RSD<5% (n=5).The method was sensitive,accu-rate and easy-controlled,and it was suitable for quantitive determination of 3,4,5-trihydroxy benzoic acid in different types of tobacco essence and flavor.
Keywords：high performance liquid chromatography(HPLC)tobacco essence and flavor345-trihy-droxy benzoic acid
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 39-42 )