DNA immobilization and hybridization on graphene/gold nanoparticles/polypyrrole nanocomposite
WANGMing-hua
YANGGuang
ZHANGYuan-chang
KANGMeng-meng
HELing-hao
FENGXiao-zhong
PENGDong-lai
ZHANGZhi-hong
Abstract：Graphene/gold nanoparticles/polypyrrole (G/AuNPs/PPy)nanocomposite was prepared by in-situ chemical oxidation method,then DNA was immobilized and hybridized on the surface of the composite. The chemical structure,element composition and surface morphology of the nanocomposite were character-ized by Fourier transform infrared spectroscopy,X-ray photoelectron spectroscopy,field emission scanning electron microscope and transmission electron microscopy,respectively.Afterwards,electrochemical cyclic voltammetry,impedance spectroscopy and electrochemical quartz crystal microbalance were used to deter-mine the variation of electrochemical properties and weight of the nanocomposite before and after DNA im-mobilization/hybridization.It demonstrated that the electrochemical activities of G/AuNPs/PPy were better than that of G/AuNPs nanocomposite.The immobilization amount of DNA on the G/AuNPs/PPy nanocom-posite was 307 ng,and aslo 357 ng of perfectly matched target DNA was detected on the surface.
Keywords：graphene/gold nanoparticles/polypyrrole composice (G/AuNPs/PPy )DNA immobilization and hybridizationelectrochemical impedanceelectrochemical biosensor
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 6-11 )