Effects of Catalpol-Tetramethylpyrazine Formula on the Learning,Memory Abilities and Brain Energy Metabolic Disorders in AD Model Mice
ZHU Mengting
DENG Chujun
LIU Lintao
CHEN Huize
YUAN Jing
MENG Shengxi
Abstract：Objective:To observe the effects of effects of Catalpol-Tetramethylpyrazine Formula on the learning,memory abilities and brain energy metabolic disorders in Alzheimer's disease(AD)model mice.Methods:Fifty 3-month-old male APPswe/PS1△E9 mice were randomly divided into the model group,the low-dose Catalpol-Tetramethylpyrazine Formula group,the medium-dose Catalpol-Tetramethylpyrazine Formula group,the high-dose Catalpol-Tetramethylpyrazine Formula group,and the Aricept group,with 10 mice in each group.Another 10 3-month-old male C57BL/6J wild-type mice were selected as the normal group.The mice in each treatment group were gavaged with the corresponding dose of the drug,while the normal group and the model group were gavaged with the same volume of normal saline,once a day,for 8 weeks.After treatment,the behavioral characteristics of the mice were evaluated through novel object recognition test,step-down test,open field test,Y maze test and elevated plus maze test.The contents of adenosine triphosphate(ATP),adenosine diphosphate(ADP)and adenosine monophosphate(AMP)in the brain tissue of the mice were detected by high-performance liquid chromatography,and the energy charge(EC)level was calculated.Results:Compared with the model group,the resolution index and step down latency in the each treatment group were prolonged,the error times decreased,the total distance of movement and the movement speed increased,the spontaneous selection accuracy rate improved,the stay time on the open arm shortened,and the contents of ATP,ADP and EC in the brain tissue of the mice increased,while the content of AMP decreased(P<0.05 or P<0.01).Compared with the low-dose Catalpol-Tetramethylpyrazine Formula group,the resolution index in the medium-dose Catalpol-Tetramethylpyrazine Formula group,the high-dose Catalpol-Tetramethylpyrazine Formula group,and the Aricept group increased,step down latency prolonged,the error times decreased,the total movement distance and movement speed increased,the spontaneous selection accuracy rate improved,the time spent on the open arm shortened,the ATP,ADP contents and EC in the brain tissue of mice increased,and the AMP content decreased(P<0.05 or P<0.01).Compared with the medium-dose Catalpol-Tetramethylpyrazine Formula group,and the Aricept group,the resolution index in the high-dose Catalpol-Tetramethylpyrazine Formula group increased,step down latency prolonged,the error times decreased,the total movement distance and movement speed increased,the spontaneous selection accuracy rate improved,the time spent on the open arm shortened,the ATP,ADP contents and EC in the brain tissue of mice increased,and the AMP content decreased(P<0.05).Conclusion:Catalpol-Tetramethylpyrazine Formula could significantly enhance the learning and memory abilities of AD model mice,and its mechanism of action might be related to improvement of energy metabolism in the mice's brain.
Keywords：Alzheimer's diseaseCatalpol-Tetramethylpyrazine Formulalearning and memory abilityenergy metabolismmiceexperimental study
Publication Date：2026-03-25
Online Publishing Date：2026-04-07(First online date of this platform, not the publication date of the document)
Pages：6( 893-898 )