The Protective Effect of Qijing Huixin Formula on the Heart Based on Network Pharmacology and Experimental Study
LI Lin
SUN Yuanlong
ZHAO Zhejun
RUAN Xiaofen
Abstract：Objective:To investigate the protective effect of Qijing Huixin Formula on the heart based on network pharmacology and basic study.Methods:The potential active components of Qijing Huixin Formula and its corresponding target sites were screened out through the drug target prediction website and by reviewing the literature.Using the disease database,the related target sites of acute myocardial infarction(AMI)were retrieved,and the intersection of the two target sites was obtained.The interaction network diagram of drug active components and target sites,the protein-protein interaction(PPI)network diagram were drawn.The gene ontology(GO)functional enrichment analysis,Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis were conducted,and molecular docking was used for verification.The therapeutic effect and mechanism of Qijing Huixin Formula in treating AMI rats were verified.Results:Qijing Huixin Formula for treating AMI involved 239 drug targets.The quercetin,kaempferol,digitonin flavonoids,β-glucoside,and Tanshinone ⅡA showed the greatest effect.Protein kinase B1(AKT1),glycogen synthase kinase 3β(GSK3β),vascular endothelial growth factor A(VEGFA),tumor necrosis factor(TNF),and interleukin 6(IL6)were the key targets.The main enriched pathways included the phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)signaling pathway,TNF signaling pathway,and apoptosis signaling pathway.The cardiac function of AMI rats,the levels of serum myocardial enzymes,lactate dehydrogenase(LDH),creatine kinase isoenzyme(CK-MB),and inflammatory factors(TNF-α,IL-6),microvascular density,the mRNA expressions of VEGFA and the markers of the PI3K/AKT/catenin β1(CTNNB1)pathway in the myocardial infarction edge zone improved by treatment of Qijing Huixin Formula(P＜0.05).Conclusion:Qijing Huixin Formula could improve myocardial injury in AMI rats by activating the PI3K/GSK3β/CTNNB1 signaling pathway in myocardial tissue and promoting angiogenesis,thereby enhancing cardiac function.
Keywords：acute myocardial infarctionQijing Huixin Formulaphosphatidylinositol 3-kinase/glycogen synthase kinase 3β/catenin β1 signaling pathwayangiogenesisratsexperimental study
Publication Date：2026-03-25
Online Publishing Date：2026-04-07(First online date of this platform, not the publication date of the document)
Pages：11( 849-859 )